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Title: Process for whole cell saccharification of lignocelluloses to sugars using a dual bioreactor system

Abstract

The present invention describes a process for saccharification of lignocelluloses to sugars using whole microbial cells, which are enriched from cultures inoculated with paper mill waste water, wood processing waste and soil. A three-member bacterial consortium is selected as a potent microbial inocula and immobilized on inedible plant fibers for biomass saccharification. The present invention further relates the design of a dual bioreactor system, with various biocarriers for enzyme immobilization and repeated use. Sugars are continuously removed eliminating end-product inhibition and consumption by cell.

Inventors:
 [1];  [2]
  1. Okemos, MI
  2. Montgomery, AL
Issue Date:
Research Org.:
Technova Corp
Sponsoring Org.:
USDOE
OSTI Identifier:
1039201
Patent Number(s):
8143040
Application Number:
12/379,569
Assignee:
Metna Co (Lansing, MI)
Patent Classifications (CPCs):
Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y02 - TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE Y02E - REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
DOE Contract Number:  
FG02-07ER84872
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Lu, Jue, and Okeke, Benedict. Process for whole cell saccharification of lignocelluloses to sugars using a dual bioreactor system. United States: N. p., 2012. Web.
Lu, Jue, & Okeke, Benedict. Process for whole cell saccharification of lignocelluloses to sugars using a dual bioreactor system. United States.
Lu, Jue, and Okeke, Benedict. Tue . "Process for whole cell saccharification of lignocelluloses to sugars using a dual bioreactor system". United States. https://www.osti.gov/servlets/purl/1039201.
@article{osti_1039201,
title = {Process for whole cell saccharification of lignocelluloses to sugars using a dual bioreactor system},
author = {Lu, Jue and Okeke, Benedict},
abstractNote = {The present invention describes a process for saccharification of lignocelluloses to sugars using whole microbial cells, which are enriched from cultures inoculated with paper mill waste water, wood processing waste and soil. A three-member bacterial consortium is selected as a potent microbial inocula and immobilized on inedible plant fibers for biomass saccharification. The present invention further relates the design of a dual bioreactor system, with various biocarriers for enzyme immobilization and repeated use. Sugars are continuously removed eliminating end-product inhibition and consumption by cell.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2012},
month = {3}
}

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Works referenced in this record:

Cellulase families and their genes
journal, September 1987


Aerobic and facultatively anaerobic cellulolytic bacteria from the gut of the termite Zootermopsis angusticollis
journal, January 2002


What is (and is not) vital to advancing cellulosic ethanol
journal, April 2007


Microbial xylanolytic systems
journal, November 1985


Investing in green and white biotech
journal, July 2006