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Title: Site-specific incorporation of redox active amino acids into proteins

Abstract

Compositions and methods of producing components of protein biosynthetic machinery that include orthogonal tRNAs, orthogonal aminoacyl-tRNA synthetases, and orthogonal pairs of tRNAs/synthetases, which incorporate redox active amino acids into proteins are provided. Methods for identifying these orthogonal pairs are also provided along with methods of producing proteins with redox active amino acids using these orthogonal pairs.

Inventors:
 [1];  [2];  [1]
  1. San Diego, CA
  2. La Jolla, CA
Issue Date:
Research Org.:
The Scripps Research Institute (LaJolla, CA)
Sponsoring Org.:
USDOE
OSTI Identifier:
1036985
Patent Number(s):
8114654
Application Number:
12/806,751
Assignee:
The Scripps Research Institute (LaJolla, CA)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
DOE Contract Number:  
FG03-00ER45812
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Alfonta, Lital, Schultz, Peter G, and Zhang, Zhiwen. Site-specific incorporation of redox active amino acids into proteins. United States: N. p., 2012. Web.
Alfonta, Lital, Schultz, Peter G, & Zhang, Zhiwen. Site-specific incorporation of redox active amino acids into proteins. United States.
Alfonta, Lital, Schultz, Peter G, and Zhang, Zhiwen. Tue . "Site-specific incorporation of redox active amino acids into proteins". United States. https://www.osti.gov/servlets/purl/1036985.
@article{osti_1036985,
title = {Site-specific incorporation of redox active amino acids into proteins},
author = {Alfonta, Lital and Schultz, Peter G and Zhang, Zhiwen},
abstractNote = {Compositions and methods of producing components of protein biosynthetic machinery that include orthogonal tRNAs, orthogonal aminoacyl-tRNA synthetases, and orthogonal pairs of tRNAs/synthetases, which incorporate redox active amino acids into proteins are provided. Methods for identifying these orthogonal pairs are also provided along with methods of producing proteins with redox active amino acids using these orthogonal pairs.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2012},
month = {2}
}

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Works referenced in this record:

Aminoacyl-tRNA synthetases: Versatile players in the changing theater of translation
journal, November 2002


Twenty-first aminoacyl-tRNA synthetase-suppressor tRNA pairs for possible use in site-specific incorporation of amino acid analogues into proteins in eukaryotes and in eubacteria
journal, January 2001


Progress toward the evolution of an organism with an expanded genetic code
journal, April 1999


Strategies for in vitro and in vivo translation with non-natural amino acids
journal, December 1996


A New Functional Suppressor tRNA/Aminoacyl−tRNA Synthetase Pair for the in Vivo Incorporation of Unnatural Amino Acids into Proteins
journal, May 2000


Programming peptidomimetic syntheses by translating genetic codes designed de novo
journal, May 2003


Site-Specific Incorporation of a Redox-Active Amino Acid into Proteins
journal, December 2003


Expanding tRNA recognition of a tRNA synthetase by a single amino acid change
journal, May 2003


Addition of the keto functional group to the genetic code of Escherichia coli
journal, December 2002


An Expanded Eukaryotic Genetic Code
journal, August 2003


A New Strategy for the Site-Specific Modification of Proteins in Vivo
journal, June 2003


A general approach for the generation of orthogonal tRNAs
journal, September 2001


Expanding the Genetic Code of Escherichia coli
journal, April 2001