Nucleic acids, compositions and uses thereof
Abstract
The subject invention provides at least one nucleic acid sequence encoding an aldouronate-utilization regulon isolated from Paenibacillus sp. strain JDR-2, a bacterium which efficiently utilizes xylan and metabolizes aldouronates (methylglucuronoxylosaccharides). The subject invention also provides a means for providing a coordinately regulated process in which xylan depolymerization and product assimilation are coupled in Paenibacillus sp. strain JDR-2 to provide a favorable system for the conversion of lignocellulosic biomass to biobased products. Additionally, the nucleic acid sequences encoding the aldouronate-utilization regulon can be used to transform other bacteria to form organisms capable of producing a desired product (e.g., ethanol, 1-butanol, acetoin, 2,3-butanediol, 1,3-propanediol, succinate, lactate, acetate, malate or alanine) from lignocellulosic biomass.
- Inventors:
-
- (Micanopy, FL)
- Gainesville, FL
- Baltimore, MD
- Issue Date:
- Research Org.:
- University of Florida Research Foundation, Inc. (Gainesville, FL)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1036512
- Patent Number(s):
- 8119367
- Application Number:
- 12/256,155
- Assignee:
- University of Florida Research Foundation, Inc. (Gainesville, FL)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
- DOE Contract Number:
- FC36-00GO10594; FC36-99GO10476
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Preston, III, James F., Chow, Virginia, Nong, Guang, Rice, John D, and John, Franz J. Nucleic acids, compositions and uses thereof. United States: N. p., 2012.
Web.
Preston, III, James F., Chow, Virginia, Nong, Guang, Rice, John D, & John, Franz J. Nucleic acids, compositions and uses thereof. United States.
Preston, III, James F., Chow, Virginia, Nong, Guang, Rice, John D, and John, Franz J. Tue .
"Nucleic acids, compositions and uses thereof". United States. https://www.osti.gov/servlets/purl/1036512.
@article{osti_1036512,
title = {Nucleic acids, compositions and uses thereof},
author = {Preston, III, James F. and Chow, Virginia and Nong, Guang and Rice, John D and John, Franz J},
abstractNote = {The subject invention provides at least one nucleic acid sequence encoding an aldouronate-utilization regulon isolated from Paenibacillus sp. strain JDR-2, a bacterium which efficiently utilizes xylan and metabolizes aldouronates (methylglucuronoxylosaccharides). The subject invention also provides a means for providing a coordinately regulated process in which xylan depolymerization and product assimilation are coupled in Paenibacillus sp. strain JDR-2 to provide a favorable system for the conversion of lignocellulosic biomass to biobased products. Additionally, the nucleic acid sequences encoding the aldouronate-utilization regulon can be used to transform other bacteria to form organisms capable of producing a desired product (e.g., ethanol, 1-butanol, acetoin, 2,3-butanediol, 1,3-propanediol, succinate, lactate, acetate, malate or alanine) from lignocellulosic biomass.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Feb 21 00:00:00 EST 2012},
month = {Tue Feb 21 00:00:00 EST 2012}
}
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