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Title: Engineered microorganisms capable of producing target compounds under anaerobic conditions

Abstract

The present invention is generally provides recombinant microorganisms comprising engineered metabolic pathways capable of producing C3-C5 alcohols under aerobic and anaerobic conditions. The invention further provides ketol-acid reductoisomerase enzymes which have been mutated or modified to increase their NADH-dependent activity or to switch the cofactor preference from NADPH to NADH and are expressed in the modified microorganisms. In addition, the invention provides isobutyraldehyde dehydrogenase enzymes expressed in modified microorganisms. Also provided are methods of producing beneficial metabolites under aerobic and anaerobic conditions by contacting a suitable substrate with the modified microorganisms of the present invention.

Inventors:
 [1];  [1];  [2];  [3];  [4];  [5];  [6]
  1. Denver, CO
  2. San Francisco, CA
  3. Parker, CO
  4. Irvine, CA
  5. Pasadena, CA
  6. La Canada, CA
Issue Date:
Research Org.:
Gevo, Inc. (Englewood, CO)
Sponsoring Org.:
USDOE
OSTI Identifier:
1034914
Patent Number(s):
8097440
Application Number:
13/269,937
Assignee:
Gevo, Inc. (Englewood, CO); California Institute of Technology (Pasadena, CA)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
DOE Contract Number:  
FG02-07ER84893
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES

Citation Formats

Buelter, Thomas, Meinhold, Peter, Feldman, Reid M. Renny, Hawkins, Andrew C, Urano, Jun, Bastian, Sabine, and Arnold, Frances. Engineered microorganisms capable of producing target compounds under anaerobic conditions. United States: N. p., 2012. Web.
Buelter, Thomas, Meinhold, Peter, Feldman, Reid M. Renny, Hawkins, Andrew C, Urano, Jun, Bastian, Sabine, & Arnold, Frances. Engineered microorganisms capable of producing target compounds under anaerobic conditions. United States.
Buelter, Thomas, Meinhold, Peter, Feldman, Reid M. Renny, Hawkins, Andrew C, Urano, Jun, Bastian, Sabine, and Arnold, Frances. Tue . "Engineered microorganisms capable of producing target compounds under anaerobic conditions". United States. https://www.osti.gov/servlets/purl/1034914.
@article{osti_1034914,
title = {Engineered microorganisms capable of producing target compounds under anaerobic conditions},
author = {Buelter, Thomas and Meinhold, Peter and Feldman, Reid M. Renny and Hawkins, Andrew C and Urano, Jun and Bastian, Sabine and Arnold, Frances},
abstractNote = {The present invention is generally provides recombinant microorganisms comprising engineered metabolic pathways capable of producing C3-C5 alcohols under aerobic and anaerobic conditions. The invention further provides ketol-acid reductoisomerase enzymes which have been mutated or modified to increase their NADH-dependent activity or to switch the cofactor preference from NADPH to NADH and are expressed in the modified microorganisms. In addition, the invention provides isobutyraldehyde dehydrogenase enzymes expressed in modified microorganisms. Also provided are methods of producing beneficial metabolites under aerobic and anaerobic conditions by contacting a suitable substrate with the modified microorganisms of the present invention.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2012},
month = {1}
}

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Works referenced in this record:

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