skip to main content
DOE Patents title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Cellulolytic enzymes, nucleic acids encoding them and methods for making and using them

Abstract

The invention provides polypeptides having any cellulolytic activity, e.g., a cellulase activity, a endoglucanase, a cellobiohydrolase, a beta-glucosidase, a xylanase, a mannanse, a .beta.-xylosidase, an arabinofuranosidase, and/or an oligomerase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In one aspect, the invention is directed to polypeptides having any cellulolytic activity, e.g., a cellulase activity, e.g., endoglucanase, cellobiohydrolase, beta-glucosidase, xylanase, mannanse, .beta.-xylosidase, arabinofuranosidase, and/or oligomerase activity, including thermostable and thermotolerant activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. In one aspect, the invention provides polypeptides having an oligomerase activity, e.g., enzymes that convert recalcitrant soluble oligomers to fermentable sugars in the saccharification of biomass. The polypeptides of the invention can be used in a variety of pharmaceutical, agricultural, food and feed processing and industrial contexts. The invention also provides compositions or products of manufacture comprising mixtures of enzymes comprising at least one enzyme of this invention.

Inventors:
 [1];  [2];  [1]
  1. San Diego, CA
  2. Emeryville, CA
Issue Date:
Research Org.:
EI DUPORN NEMOURS & CO
Sponsoring Org.:
USDOE
OSTI Identifier:
1034636
Patent Number(s):
8101393
Application Number:
12/278,958
Assignee:
BP Corporation North America Inc. (Houston, TX)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C - CHEMISTRY C12 - BIOCHEMISTRY C12Y - ENZYMES
DOE Contract Number:  
FC36-03GO13146
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES

Citation Formats

Gray, Kevin A, Zhao, Lishan, and Cayouette, Michelle H. Cellulolytic enzymes, nucleic acids encoding them and methods for making and using them. United States: N. p., 2012. Web.
Gray, Kevin A, Zhao, Lishan, & Cayouette, Michelle H. Cellulolytic enzymes, nucleic acids encoding them and methods for making and using them. United States.
Gray, Kevin A, Zhao, Lishan, and Cayouette, Michelle H. Tue . "Cellulolytic enzymes, nucleic acids encoding them and methods for making and using them". United States. https://www.osti.gov/servlets/purl/1034636.
@article{osti_1034636,
title = {Cellulolytic enzymes, nucleic acids encoding them and methods for making and using them},
author = {Gray, Kevin A and Zhao, Lishan and Cayouette, Michelle H},
abstractNote = {The invention provides polypeptides having any cellulolytic activity, e.g., a cellulase activity, a endoglucanase, a cellobiohydrolase, a beta-glucosidase, a xylanase, a mannanse, a .beta.-xylosidase, an arabinofuranosidase, and/or an oligomerase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In one aspect, the invention is directed to polypeptides having any cellulolytic activity, e.g., a cellulase activity, e.g., endoglucanase, cellobiohydrolase, beta-glucosidase, xylanase, mannanse, .beta.-xylosidase, arabinofuranosidase, and/or oligomerase activity, including thermostable and thermotolerant activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. In one aspect, the invention provides polypeptides having an oligomerase activity, e.g., enzymes that convert recalcitrant soluble oligomers to fermentable sugars in the saccharification of biomass. The polypeptides of the invention can be used in a variety of pharmaceutical, agricultural, food and feed processing and industrial contexts. The invention also provides compositions or products of manufacture comprising mixtures of enzymes comprising at least one enzyme of this invention.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2012},
month = {1}
}

Patent:

Save / Share:

Works referenced in this record:

Semi-rational approaches to engineering enzyme activity: combining the benefits of directed evolution and rational design
journal, August 2005


Conversion of a β-Ketoacyl Synthase to a Malonyl Decarboxylase by Replacement of the Active-Site Cysteine with Glutamine
journal, September 1999


FUNCTIONAL GENOMICS: Expression Cloning in the Test Tube
journal, August 1997


Activity studies of eight purified cellulases: Specificity, synergism, and binding domain effects
journal, October 1993


An Evolving Hierarchical Family Classification for Glycosyltransferases
journal, April 2003


Trends in biochemistry and enzymology of cellulose degradation
journal, November 1990


Developments in Directed Evolution for Improving Enzyme Functions
journal, August 2007


Structural and sequence-based classification of glycoside hydrolases
journal, October 1997


Directed evolution of an aspartate aminotransferase with new substrate specificities
journal, May 1998


Prediction of protein function from protein sequence and structure
journal, January 1999


Toward an aggregated understanding of enzymatic hydrolysis of cellulose: Noncomplexed cellulase systems
journal, November 2004


High-Resolution Capillary Gel Electrophoresis of Reducing Oligosaccharides Labeled with 1-Aminopyrene-3,6,8-trisulfonate
journal, January 1996


Nucleotide sequence of thecelA gene encoding a cellodextrinase ofRuminococcus flavefaciens FD-1
journal, July 1990


Production and characterization of β-glucosidases from different Aspergillus strains
journal, July 2001


Cellulase assay methods: a review
journal, October 1988


Roles of the Catalytic Domain and Two Cellulose Binding Domains of Thermomonospora fusca E4 in Cellulose Hydrolysis
journal, January 1998


Microbial Cellulose Utilization: Fundamentals and Biotechnology
journal, September 2002


A Novel, High Performance Enzyme for Starch Liquefaction: DISCOVERY AND OPTIMIZATION OF A LOW pH, THERMOSTABLE α-AMYLASE
journal, May 2002


Hemicellulose bioconversion
journal, May 2003


Melamine Deaminase and Atrazine Chlorohydrolase: 98 Percent Identical but Functionally Different
journal, April 2001


Carbohydrate-binding modules: fine-tuning polysaccharide recognition
journal, September 2004


Catalytic Plasticity of Fatty Acid Modification Enzymes Underlying Chemical Diversity of Plant Lipids
journal, November 1998


The Topology of the Substrate Binding Clefts of Glycosyl Hydrolase Family 10 Xylanases Are Not Conserved
journal, November 1998


Structure and mechanism of endo/exocellulase E4 from Thermomonospora fusca
journal, October 1997


Basic local alignment search tool
journal, October 1990


Cellulase for commodity products from cellulosic biomass
journal, August 1999


A carbohydrate binding module as a diversity-carrying scaffold
journal, February 2004


The use of selection in recovery of transgenic targets for mutation analysis
journal, February 1993


Nucleotide sequence of the Ruminococcus albus SY3 endoglucanase genes ce1A and ce1B
journal, September 1990


Methods for measuring cellulase activities
journal, January 1988


Comparison of modular and non-modular xylanases as carrier proteins for the efficient secretion of heterologous proteins from Penicillium funiculosum
journal, December 2002


Engineered xyloglucan specificity in a carbohydrate-binding module
journal, July 2006


Kinetic Measurements of Cellulase Activity on Insoluble Substrates Using Disodium 2,2' Bicinchoninate
journal, September 1998


OIL OUTLOOK:USGS Optimistic on World Oil Prospects
journal, July 2000


Biochemical characterization and mode of action of a thermostable endoglucanase purified from Thermoascus aurantiacus
journal, August 2002


A cellulase assay coupled to cellobiose dehydrogenase
journal, June 1985


Estimation of cellulase activity using a glucose-oxidase-Cu(II) reducing assay for glucose
journal, December 1991


A new thermostable endoglucanase,Acidothermus cellulolyticus E1: Synergism withTrichoderma reesei CBH I and comparison to Thermomonospora fusca E5
journal, March 1994


Microbial hemicellulases
journal, June 2003


Ethanol fermentation from biomass resources: current state and prospects
journal, December 2005


Biotechnological applications of phage and cell display
journal, February 2001


λ ZAP: a bacteriophage λ expression vector with in vivo excision properties
journal, January 1988


Consolidated bioprocessing of cellulosic biomass: an update
journal, October 2005


Sensitive assay for cellulase and dextranase
journal, June 1976


The Cellulosome: The Exocellular Organelle of Clostridium
journal, October 1993


Protein tolerance to random amino acid change
journal, June 2004


Cellulase, Clostridia, and Ethanol
journal, March 2005


Crystal structure of the catalytic core domain of the family 6 cellobiohydrolase II, Cel6A, from Humicola insolens, at 1.92 Å resolution
journal, January 1999