Title: Cellulolytic enzymes, nucleic acids encoding them and methods for making and using them

Abstract

The invention provides polypeptides having any cellulolytic activity, e.g., a cellulase activity, a endoglucanase, a cellobiohydrolase, a beta-glucosidase, a xylanase, a mannanse, a .beta.-xylosidase, an arabinofuranosidase, and/or an oligomerase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In one aspect, the invention is directed to polypeptides having any cellulolytic activity, e.g., a cellulase activity, e.g., endoglucanase, cellobiohydrolase, beta-glucosidase, xylanase, mannanse, .beta.-xylosidase, arabinofuranosidase, and/or oligomerase activity, including thermostable and thermotolerant activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. In one aspect, the invention provides polypeptides having an oligomerase activity, e.g., enzymes that convert recalcitrant soluble oligomers to fermentable sugars in the saccharification of biomass. The polypeptides of the invention can be used in a variety of pharmaceutical, agricultural, food and feed processing and industrial contexts. The invention also provides compositions or products of manufacture comprising mixtures of enzymes comprising at least one enzyme of this invention.

Inventors:

Gray, Kevin A ^[1]; Zhao, Lishan ^[2]; Cayouette, Michelle H ^[1]

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+ Show Inventor Affiliations

1. San Diego, CA
2. Emeryville, CA

Issue Date:

Tue Jan 24 00:00:00 EST 2012

Research Org.:

EI DUPORN NEMOURS & CO

Sponsoring Org.:

USDOE

OSTI Identifier:

1034636

Patent Number(s):

8101393

Application Number:

12/278,958

Assignee:

BP Corporation North America Inc. (Houston, TX)

Patent Classifications (CPCs):

A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61P - SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

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A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61P - SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS

A61P43/00 - Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

C12N9/2402 - {hydrolysing O- and S- glycosyl compounds
C12N9/2405 - {Glucanases}
C12N9/2437 - {Cellulases
C12N9/248 - {Xylanases}

C - CHEMISTRY C12 - BIOCHEMISTRY C12Y - ENZYMES

C12Y302/01004 - Cellulase
C12Y302/01006 - Endo-1,3
C12Y302/01008 - Endo-1,4-beta-xylanase
C12Y302/01021 - Beta-glucosidase
C12Y302/01023 - Beta-galactosidase
C12Y302/01039 - Glucan endo-1,3-beta-D-glucosidase
C12Y302/01055 - Alpha-N-arabinofuranosidase
C12Y302/01091 - Cellulose 1,4-beta-cellobiosidase

Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y02 - TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE Y02E - REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION

Y02E50/10 - Biofuels

Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y10 - TECHNICAL SUBJECTS COVERED BY FORMER USPC Y10T - TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION

Y10T428/249921 - Web or sheet containing structurally defined element or component

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DOE Contract Number:  

FC36-03GO13146

Resource Type:

Patent

Country of Publication:

United States

Language:

English

Subject:

60 APPLIED LIFE SCIENCES