High-throughput method for optimum solubility screening for homogeneity and crystallization of proteins
Abstract
An optimum solubility screen in which a panel of buffers and many additives are provided in order to obtain the most homogeneous and monodisperse protein condition for protein crystallization. The present methods are useful for proteins that aggregate and cannot be concentrated prior to setting up crystallization screens. A high-throughput method using the hanging-drop method and vapor diffusion equilibrium and a panel of twenty-four buffers is further provided. Using the present methods, 14 poorly behaving proteins have been screened, resulting in 11 of the proteins having highly improved dynamic light scattering results allowing concentration of the proteins, and 9 were crystallized.
- Inventors:
-
- Moraga, CA
- Walnut Creek, CA
- Issue Date:
- Research Org.:
- Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1034608
- Patent Number(s):
- 8105983
- Application Number:
- 11/393,209
- Assignee:
- The Regents of the University of California (Oakland, CA)
- Patent Classifications (CPCs):
-
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- DOE Contract Number:
- AC02-05CH11231
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Kim, Sung-Hou, Kim, Rosalind, and Jancarik, Jamila. High-throughput method for optimum solubility screening for homogeneity and crystallization of proteins. United States: N. p., 2012.
Web.
Kim, Sung-Hou, Kim, Rosalind, & Jancarik, Jamila. High-throughput method for optimum solubility screening for homogeneity and crystallization of proteins. United States.
Kim, Sung-Hou, Kim, Rosalind, and Jancarik, Jamila. Tue .
"High-throughput method for optimum solubility screening for homogeneity and crystallization of proteins". United States. https://www.osti.gov/servlets/purl/1034608.
@article{osti_1034608,
title = {High-throughput method for optimum solubility screening for homogeneity and crystallization of proteins},
author = {Kim, Sung-Hou and Kim, Rosalind and Jancarik, Jamila},
abstractNote = {An optimum solubility screen in which a panel of buffers and many additives are provided in order to obtain the most homogeneous and monodisperse protein condition for protein crystallization. The present methods are useful for proteins that aggregate and cannot be concentrated prior to setting up crystallization screens. A high-throughput method using the hanging-drop method and vapor diffusion equilibrium and a panel of twenty-four buffers is further provided. Using the present methods, 14 poorly behaving proteins have been screened, resulting in 11 of the proteins having highly improved dynamic light scattering results allowing concentration of the proteins, and 9 were crystallized.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2012},
month = {1}
}
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