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Title: Method for redesign of microbial production systems

Abstract

A computer-assisted method for identifying functionalities to add to an organism-specific metabolic network to enable a desired biotransformation in a host includes accessing reactions from a universal database to provide stoichiometric balance, identifying at least one stoichiometrically balanced pathway at least partially based on the reactions and a substrate to minimize a number of non-native functionalities in the production host, and incorporating the at least one stoichiometrically balanced pathway into the host to provide the desired biotransformation. A representation of the metabolic network as modified can be stored.

Inventors:
 [1];  [2];  [2]
  1. State College, PA
  2. San Diego, CA
Issue Date:
Research Org.:
Genomatica Inc.
Sponsoring Org.:
USDOE
OSTI Identifier:
1034356
Patent Number(s):
8108152
Application Number:
12/886,649
Assignee:
The Penn State Research Foundation (University Park, PA)
Patent Classifications (CPCs):
G - PHYSICS G16 - INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS G16B - BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
DOE Contract Number:  
FG03-01ER25499
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES

Citation Formats

Maranas, Costas D, Burgard, Anthony P, and Pharkya, Priti. Method for redesign of microbial production systems. United States: N. p., 2012. Web.
Maranas, Costas D, Burgard, Anthony P, & Pharkya, Priti. Method for redesign of microbial production systems. United States.
Maranas, Costas D, Burgard, Anthony P, and Pharkya, Priti. Tue . "Method for redesign of microbial production systems". United States. https://www.osti.gov/servlets/purl/1034356.
@article{osti_1034356,
title = {Method for redesign of microbial production systems},
author = {Maranas, Costas D and Burgard, Anthony P and Pharkya, Priti},
abstractNote = {A computer-assisted method for identifying functionalities to add to an organism-specific metabolic network to enable a desired biotransformation in a host includes accessing reactions from a universal database to provide stoichiometric balance, identifying at least one stoichiometrically balanced pathway at least partially based on the reactions and a substrate to minimize a number of non-native functionalities in the production host, and incorporating the at least one stoichiometrically balanced pathway into the host to provide the desired biotransformation. A representation of the metabolic network as modified can be stored.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2012},
month = {1}
}

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