DOE Patents title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Method and apparatus for sustaining viability of biological cells on a substrate

Abstract

A method for the transient transformation of a living biological cell having an intact cell membrane defining an intracellular domain, and an apparatus for the transient transformation of biological cells. The method and apparatus include introducing a compartmentalized extracellular component fixedly attached to a cellular penetrant structure to the intracellular domain of the cell, wherein the cell is fixed in a predetermined location and wherein the component is expressed within in the cell while being retained within the compartment and wherein the compartment restricts the mobility and interactions of the component within the cell and prevents transference of the component to the cell.

Inventors:
 [1];  [2];  [3]
  1. Greenback, TN
  2. Oak Ridge, TN
  3. Knoxville, TN
Issue Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1034300
Patent Number(s):
8076124
Application Number:
11/624,040
Assignee:
UT-Battelle, LLC (Oak Ridge, TN)
Patent Classifications (CPCs):
B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01L - CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y10 - TECHNICAL SUBJECTS COVERED BY FORMER USPC Y10S - TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
DOE Contract Number:  
AC05-00OR22725
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

McKnight, Timothy E, Melechko, Anatoli V, and Simpson, Michael L. Method and apparatus for sustaining viability of biological cells on a substrate. United States: N. p., 2011. Web.
McKnight, Timothy E, Melechko, Anatoli V, & Simpson, Michael L. Method and apparatus for sustaining viability of biological cells on a substrate. United States.
McKnight, Timothy E, Melechko, Anatoli V, and Simpson, Michael L. Tue . "Method and apparatus for sustaining viability of biological cells on a substrate". United States. https://www.osti.gov/servlets/purl/1034300.
@article{osti_1034300,
title = {Method and apparatus for sustaining viability of biological cells on a substrate},
author = {McKnight, Timothy E and Melechko, Anatoli V and Simpson, Michael L},
abstractNote = {A method for the transient transformation of a living biological cell having an intact cell membrane defining an intracellular domain, and an apparatus for the transient transformation of biological cells. The method and apparatus include introducing a compartmentalized extracellular component fixedly attached to a cellular penetrant structure to the intracellular domain of the cell, wherein the cell is fixed in a predetermined location and wherein the component is expressed within in the cell while being retained within the compartment and wherein the compartment restricts the mobility and interactions of the component within the cell and prevents transference of the component to the cell.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2011},
month = {12}
}

Works referenced in this record:

A galinstan expansion femtosyringe for microinjection of eukaryotic organelles and prokaryotes
journal, September 1999


Direct haplotyping of kilobase-size DNA using carbon nanotube probes
journal, July 2000


Fabrication and Characterization of Vertically Aligned Carbon Nanotubes on Silicon Substrates Using Porous Alumina Nanotemplates
journal, April 2002


Carbon nanotube array-based biosensor
journal, January 2003


Effects of spatial separation on the growth of vertically aligned carbon nanofibers produced by plasma-enhanced chemical vapor deposition
journal, January 2002


Major limitations in the use of cationic liposomes for DNA delivery
journal, March 1998


Structural and functional imaging with carbon nanotube AFM probes
journal, January 2001


Block and Graft Copolymers and Nanogel™ Copolymer Networks for DNA Delivery into Cell
journal, January 2000


Microarrays of cells expressing defined cDNAs
journal, May 2001


Laterally Stabilized Complexes of DNA with Linear Reducible Polycations:  Strategy for Triggered Intracellular Activation of DNA Delivery Vectors
journal, January 2002


The use of Chinese hamster ovary cells to quantify specific locus mutation and to determine mutagenicity of chemicals: A report of the GENE-TOX program
journal, March 1981


Micromachined interfaces: new approaches in cell immunoisolation and biomolecular separation
journal, October 2000


Construction of a genetic toggle switch in Escherichia coli
journal, January 2000


Electrochemically induced adsorption of radio-labeled DNA on gold and HOPG substrates for STM investigations
journal, December 1991


Tracking Gene Expression after DNA Delivery Using Spatially Indexed Nanofiber Arrays
journal, July 2004


Field-emission studies of smooth and nanostructured carbon films
journal, August 1999


The emerging field of nanotube biotechnology
journal, January 2003


DNA-functionalized single-walled carbon nanotubes
journal, September 2002


Modelling cellular behaviour
journal, January 2001


Gene expression using an ultrathin needle enabling accurate displacement and low invasiveness
journal, July 2005


Fabrication of Poly(ethylene glycol) Hydrogel Microstructures Using Photolithography
journal, September 2001


Covalently functionalized nanotubes as nanometre- sized probes in chemistry and biology
journal, July 1998


Quantum Dot Bioconjugates for Ultrasensitive Nonisotopic Detection
journal, September 1998


Engineered gene circuits
journal, November 2002


Semiconductor Nanocrystals as Fluorescent Biological Labels
patent, September 1998


Natural alleles at a tomato fruit size quantitative trait locus differ by heterochronic regulatory mutations
journal, October 2002


Shaping carbon nanostructures by controlling the synthesis process
journal, August 2001


Large-Scale Synthesis of Aligned Carbon Nanotubes
journal, December 1996


Microfabricated Microneedles for Gene and Drug Delivery
journal, August 2000


Patterned growth of individual and multiple vertically aligned carbon nanofibers
journal, June 2000


Synthetic DNA delivery systems
journal, January 2000


Nanotube Nanotweezers
journal, December 1999


Whole-cell biocomputing
journal, August 2001


Combinatorial Synthesis of Genetic Networks
journal, May 2002


Preparation of Nucleic Acid Functionalized Carbon Nanotube Arrays
journal, October 2002


Individually addressable vertically aligned carbon nanofiber-based electrochemical probes
journal, March 2002


A molecular delivery system by using AFM and nanoneedle
journal, April 2005


Field emission properties of different forms of carbon
journal, June 2001


Temporally resolved catecholamine spikes correspond to single vesicle release from individual chromaffin cells.
journal, December 1991


Carbon nanotube-enhanced electrochemical DNA biosensor for DNA hybridization detection
journal, January 2003


A synthetic oscillatory network of transcriptional regulators
journal, January 2000


Alignment mechanism of carbon nanofibers produced by plasma-enhanced chemical-vapor deposition
journal, October 2001


Remote electronic control of DNA hybridization through inductive coupling to an attached metal nanocrystal antenna
journal, January 2002