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Title: Method and apparatus for sustaining viability of biological cells on a substrate

Abstract

A method for the transient transformation of a living biological cell having an intact cell membrane defining an intracellular domain, and an apparatus for the transient transformation of biological cells. The method and apparatus include introducing a compartmentalized extracellular component fixedly attached to a cellular penetrant structure to the intracellular domain of the cell, wherein the cell is fixed in a predetermined location and wherein the component is expressed within in the cell while being retained within the compartment and wherein the compartment restricts the mobility and interactions of the component within the cell and prevents transference of the component to the cell.

Inventors:
 [1];  [2];  [3]
  1. Greenback, TN
  2. Oak Ridge, TN
  3. Knoxville, TN
Issue Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1034300
Patent Number(s):
8076124
Application Number:
11/624,040
Assignee:
UT-Battelle, LLC (Oak Ridge, TN)
Patent Classifications (CPCs):
B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01L - CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y10 - TECHNICAL SUBJECTS COVERED BY FORMER USPC Y10S - TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
DOE Contract Number:  
AC05-00OR22725
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

McKnight, Timothy E, Melechko, Anatoli V, and Simpson, Michael L. Method and apparatus for sustaining viability of biological cells on a substrate. United States: N. p., 2011. Web.
McKnight, Timothy E, Melechko, Anatoli V, & Simpson, Michael L. Method and apparatus for sustaining viability of biological cells on a substrate. United States.
McKnight, Timothy E, Melechko, Anatoli V, and Simpson, Michael L. Tue . "Method and apparatus for sustaining viability of biological cells on a substrate". United States. https://www.osti.gov/servlets/purl/1034300.
@article{osti_1034300,
title = {Method and apparatus for sustaining viability of biological cells on a substrate},
author = {McKnight, Timothy E and Melechko, Anatoli V and Simpson, Michael L},
abstractNote = {A method for the transient transformation of a living biological cell having an intact cell membrane defining an intracellular domain, and an apparatus for the transient transformation of biological cells. The method and apparatus include introducing a compartmentalized extracellular component fixedly attached to a cellular penetrant structure to the intracellular domain of the cell, wherein the cell is fixed in a predetermined location and wherein the component is expressed within in the cell while being retained within the compartment and wherein the compartment restricts the mobility and interactions of the component within the cell and prevents transference of the component to the cell.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2011},
month = {12}
}

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