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Title: Method for selective immobilization of macromolecules on self assembled monolayer surfaces

Abstract

Disclosed is a method for selective chemical binding and immobilization of macromolecules on solid supports in conjunction with self-assembled monolayer (SAM) surfaces. Immobilization involves selective binding of peptides and other macromolecules to SAM surfaces using reactive landing (RL) of mass-selected, gas phase ions. SAM surfaces provide a simple and convenient platform for tailoring chemical properties of a variety of substrates. The invention finds applications in biochemistry ranging from characterization of molecular recognition events at the amino acid level and identification of biologically active motifs in proteins, to development of novel biosensors and substrates for stimulated protein and cell adhesion.

Inventors:
 [1];  [2]
  1. Richland, WA
  2. Billerica, MA
Issue Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1032634
Patent Number(s):
8067053
Application Number:
12/151,945
Assignee:
Battelle Memorial Institute (Richland, WA)
Patent Classifications (CPCs):
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES
DOE Contract Number:  
AC06-76RL01830
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY

Citation Formats

Laskin, Julia, and Wang, Peng. Method for selective immobilization of macromolecules on self assembled monolayer surfaces. United States: N. p., 2011. Web.
Laskin, Julia, & Wang, Peng. Method for selective immobilization of macromolecules on self assembled monolayer surfaces. United States.
Laskin, Julia, and Wang, Peng. Tue . "Method for selective immobilization of macromolecules on self assembled monolayer surfaces". United States. https://www.osti.gov/servlets/purl/1032634.
@article{osti_1032634,
title = {Method for selective immobilization of macromolecules on self assembled monolayer surfaces},
author = {Laskin, Julia and Wang, Peng},
abstractNote = {Disclosed is a method for selective chemical binding and immobilization of macromolecules on solid supports in conjunction with self-assembled monolayer (SAM) surfaces. Immobilization involves selective binding of peptides and other macromolecules to SAM surfaces using reactive landing (RL) of mass-selected, gas phase ions. SAM surfaces provide a simple and convenient platform for tailoring chemical properties of a variety of substrates. The invention finds applications in biochemistry ranging from characterization of molecular recognition events at the amino acid level and identification of biologically active motifs in proteins, to development of novel biosensors and substrates for stimulated protein and cell adhesion.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2011},
month = {11}
}

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Works referenced in this record:

Preparative Soft and Reactive Landing of Gas-Phase Ions on Plasma-Treated Metal Surfaces
journal, August 2005


Reactive landing of peptide ions on self-assembled monolayer surfaces: an alternative approach for covalent immobilization of peptides on surfaces
journal, January 2008


Soft-Landing of Peptides onto Self-Assembled Monolayer Surfaces
journal, February 2006


Design and Performance of an Instrument for Soft Landing of Biomolecular Ions on Surfaces
journal, September 2007


Covalent Immobilization of Peptides on Self-Assembled Monolayer Surfaces Using Soft-Landing of Mass-Selected Ions
journal, July 2007


First Observation of Charge Reduction and Desorption Kinetics of Multiply Protonated Peptides Soft Landed onto Self-Assembled Monolayer Surfaces
journal, December 2007


Soft-landing of peptide ions onto self-assembled monolayer surfaces: an overview
journal, January 2008


Ion/surface reactions and ion soft-landing
journal, January 2005


Preparation and in Situ Characterization of Surfaces Using Soft Landing in a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer
journal, June 2005


Charge retention by peptide ions soft-landed onto self-assembled monolayer surfaces
journal, September 2007


Preparative Soft and Reactive Landing of Multiply Charged Protein Ions on a Plasma-Treated Metal Surface
journal, August 2005