Method for selective immobilization of macromolecules on self assembled monolayer surfaces

Title: Method for selective immobilization of macromolecules on self assembled monolayer surfaces

Abstract

Disclosed is a method for selective chemical binding and immobilization of macromolecules on solid supports in conjunction with self-assembled monolayer (SAM) surfaces. Immobilization involves selective binding of peptides and other macromolecules to SAM surfaces using reactive landing (RL) of mass-selected, gas phase ions. SAM surfaces provide a simple and convenient platform for tailoring chemical properties of a variety of substrates. The invention finds applications in biochemistry ranging from characterization of molecular recognition events at the amino acid level and identification of biologically active motifs in proteins, to development of novel biosensors and substrates for stimulated protein and cell adhesion.

Inventors:

Laskin, Julia ^[1]; Wang, Peng ^[2]

Richland, WA
Billerica, MA

Issue Date:: 2011-11-29

Research Org.:: Pacific Northwest National Lab. (PNNL), Richland, WA (United States)

Sponsoring Org.:: USDOE

OSTI Identifier:: 1032634

Patent Number(s):: 8067053

Application Number:: 12/151,945

Assignee:: Battelle Memorial Institute (Richland, WA)

Patent Classifications (CPCs):: G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES

C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES

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G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
G01N33/5432 - {Liposomes or microcapsules}

C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES
C07K17/06 - attached to the carrier via a bridging agent

G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
G01N2610/00 - Assays involving self-assembled monolayers [SAMs]

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DOE Contract Number:: AC06-76RL01830

Resource Type:: Patent

Country of Publication:: United States

Language:: English

Subject:: 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY

Citation Formats


                    Laskin, Julia, and Wang, Peng. Method for selective immobilization of macromolecules on self assembled monolayer surfaces.  United States: N. p., 2011. 
        Web.

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                    Laskin, Julia, & Wang, Peng. Method for selective immobilization of macromolecules on self assembled monolayer surfaces.  United States.

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                    Laskin, Julia, and Wang, Peng. Tue .  
        "Method for selective immobilization of macromolecules on self assembled monolayer surfaces".  United States.  https://www.osti.gov/servlets/purl/1032634.

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@article{osti_1032634,

  title        = {Method for selective immobilization of macromolecules on self assembled monolayer surfaces},

  author       = {Laskin, Julia and Wang, Peng},

  abstractNote = {Disclosed is a method for selective chemical binding and immobilization of macromolecules on solid supports in conjunction with self-assembled monolayer (SAM) surfaces. Immobilization involves selective binding of peptides and other macromolecules to SAM surfaces using reactive landing (RL) of mass-selected, gas phase ions. SAM surfaces provide a simple and convenient platform for tailoring chemical properties of a variety of substrates. The invention finds applications in biochemistry ranging from characterization of molecular recognition events at the amino acid level and identification of biologically active motifs in proteins, to development of novel biosensors and substrates for stimulated protein and cell adhesion.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {2011},

  month        = {11}

}

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Works referenced in this record:

Preparative Soft and Reactive Landing of Gas-Phase Ions on Plasma-Treated Metal Surfaces
journal, August 2005

Volný, Michael; Elam, W. Timothy; Ratner, Buddy D.
Analytical Chemistry, Vol. 77, Issue 15
https://doi.org/10.1021/ac0505019

A Strategy for the Generation of Surfaces Presenting Ligands for Studies of Binding Based on an Active Ester as a Common Reactive Intermediate: A Surface Plasmon Resonance Study
journal, February 1999

Lahiri, Joydeep; Isaacs, Lyle; Tien, Joe
Analytical Chemistry, Vol. 71, Issue 4
https://doi.org/10.1021/ac980959t

Reactive landing of peptide ions on self-assembled monolayer surfaces: an alternative approach for covalent immobilization of peptides on surfaces
journal, January 2008

Wang, Peng; Hadjar, Omar; Gassman, Paul L.
Physical Chemistry Chemical Physics, Vol. 10, Issue 11
https://doi.org/10.1039/b717617a

Soft-Landing of Peptides onto Self-Assembled Monolayer Surfaces ^†
journal, February 2006

Alvarez, Jormarie; Futrell, Jean H.; Laskin, Julia
The Journal of Physical Chemistry A, Vol. 110, Issue 4
https://doi.org/10.1021/jp0555044

Design and Performance of an Instrument for Soft Landing of Biomolecular Ions on Surfaces
journal, September 2007

Hadjar, Omar; Wang, Peng; Futrell, Jean H.
Analytical Chemistry, Vol. 79, Issue 17
https://doi.org/10.1021/ac070600h

Covalent Immobilization of Peptides on Self-Assembled Monolayer Surfaces Using Soft-Landing of Mass-Selected Ions
journal, July 2007

Wang, Peng; Hadjar, Omar; Laskin, Julia
Journal of the American Chemical Society, Vol. 129, Issue 28
https://doi.org/10.1021/ja071804i

Covalent immobilization of native biomolecules onto Au(111) via N-hydroxysuccinimide ester functionalized self-assembled monolayers for scanning probe microscopy
journal, May 1996

Wagner, P.; Hegner, M.; Kernen, P.
Biophysical Journal, Vol. 70, Issue 5
https://doi.org/10.1016/S0006-3495(96)79810-7

First Observation of Charge Reduction and Desorption Kinetics of Multiply Protonated Peptides Soft Landed onto Self-Assembled Monolayer Surfaces
journal, December 2007

Hadjar, Omar; Futrell, Jean H.; Laskin, Julia
The Journal of Physical Chemistry C, Vol. 111, Issue 49
https://doi.org/10.1021/jp075293y

Soft-landing of peptide ions onto self-assembled monolayer surfaces: an overview
journal, January 2008

Laskin, Julia; Wang, Peng; Hadjar, Omar
Phys. Chem. Chem. Phys., Vol. 10, Issue 8
https://doi.org/10.1039/B712710C

Ion/surface reactions and ion soft-landing
journal, January 2005

Gologan, Bogdan; Green, Jason R.; Alvarez, Jormarie
Physical Chemistry Chemical Physics, Vol. 7, Issue 7
https://doi.org/10.1039/b418056a

Preparation and in Situ Characterization of Surfaces Using Soft Landing in a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer
journal, June 2005

Alvarez, Jormarie; Cooks, R. Graham; Barlow, S. E.
Analytical Chemistry, Vol. 77, Issue 11
https://doi.org/10.1021/ac0481349

Charge retention by peptide ions soft-landed onto self-assembled monolayer surfaces
journal, September 2007

Laskin, Julia; Wang, Peng; Hadjar, Omar
International Journal of Mass Spectrometry, Vol. 265, Issue 2-3
https://doi.org/10.1016/j.ijms.2007.02.013

Preparative Soft and Reactive Landing of Multiply Charged Protein Ions on a Plasma-Treated Metal Surface
journal, August 2005

Volný, Michael; Elam, W. Timothy; Branca, Andrew
Analytical Chemistry, Vol. 77, Issue 15, p. 4890-4896
https://doi.org/10.1021/ac0507136

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Similar Records

Title: Method for selective immobilization of macromolecules on self assembled monolayer surfaces

Abstract

Citation Formats

Preparative Soft and Reactive Landing of Gas-Phase Ions on Plasma-Treated Metal Surfaces journal, August 2005

A Strategy for the Generation of Surfaces Presenting Ligands for Studies of Binding Based on an Active Ester as a Common Reactive Intermediate: A Surface Plasmon Resonance Study journal, February 1999

Reactive landing of peptide ions on self-assembled monolayer surfaces: an alternative approach for covalent immobilization of peptides on surfaces journal, January 2008

Soft-Landing of Peptides onto Self-Assembled Monolayer Surfaces † journal, February 2006

Design and Performance of an Instrument for Soft Landing of Biomolecular Ions on Surfaces journal, September 2007

Covalent Immobilization of Peptides on Self-Assembled Monolayer Surfaces Using Soft-Landing of Mass-Selected Ions journal, July 2007

Covalent immobilization of native biomolecules onto Au(111) via N-hydroxysuccinimide ester functionalized self-assembled monolayers for scanning probe microscopy journal, May 1996

First Observation of Charge Reduction and Desorption Kinetics of Multiply Protonated Peptides Soft Landed onto Self-Assembled Monolayer Surfaces journal, December 2007

Soft-landing of peptide ions onto self-assembled monolayer surfaces: an overview journal, January 2008

Ion/surface reactions and ion soft-landing journal, January 2005

Preparation and in Situ Characterization of Surfaces Using Soft Landing in a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer journal, June 2005

Charge retention by peptide ions soft-landed onto self-assembled monolayer surfaces journal, September 2007

Preparative Soft and Reactive Landing of Multiply Charged Protein Ions on a Plasma-Treated Metal Surface journal, August 2005

Preparative Soft and Reactive Landing of Gas-Phase Ions on Plasma-Treated Metal Surfaces
journal, August 2005

A Strategy for the Generation of Surfaces Presenting Ligands for Studies of Binding Based on an Active Ester as a Common Reactive Intermediate: A Surface Plasmon Resonance Study
journal, February 1999

Reactive landing of peptide ions on self-assembled monolayer surfaces: an alternative approach for covalent immobilization of peptides on surfaces
journal, January 2008

Soft-Landing of Peptides onto Self-Assembled Monolayer Surfaces ^†
journal, February 2006

Design and Performance of an Instrument for Soft Landing of Biomolecular Ions on Surfaces
journal, September 2007

Covalent Immobilization of Peptides on Self-Assembled Monolayer Surfaces Using Soft-Landing of Mass-Selected Ions
journal, July 2007

Covalent immobilization of native biomolecules onto Au(111) via N-hydroxysuccinimide ester functionalized self-assembled monolayers for scanning probe microscopy
journal, May 1996

First Observation of Charge Reduction and Desorption Kinetics of Multiply Protonated Peptides Soft Landed onto Self-Assembled Monolayer Surfaces
journal, December 2007

Soft-landing of peptide ions onto self-assembled monolayer surfaces: an overview
journal, January 2008

Ion/surface reactions and ion soft-landing
journal, January 2005

Preparation and in Situ Characterization of Surfaces Using Soft Landing in a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer
journal, June 2005

Charge retention by peptide ions soft-landed onto self-assembled monolayer surfaces
journal, September 2007

Preparative Soft and Reactive Landing of Multiply Charged Protein Ions on a Plasma-Treated Metal Surface
journal, August 2005