Title: Compositions of orthogonal lysyl-tRNA and aminoacyl-tRNA synthetase pairs and uses thereof

Abstract

Compositions and methods of producing components of protein biosynthetic machinery that include orthogonal lysyl-tRNAs, orthogonal lysyl-aminoacyl-tRNA synthetases, and orthogonal pairs of lysyl-tRNAs/synthetases, which incorporate homoglutamines into proteins are provided in response to a four base codon. Methods for identifying these orthogonal pairs are also provided along with methods of producing proteins with homoglutamines using these orthogonal pairs.

Inventors:

Anderson, J Christopher ^[1]; Wu, Ning ^[2]; Santoro, Stephen ^[3]; Schultz, Peter G ^[4]

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+ Show Inventor Affiliations

1. San Francisco, CA
2. Brookline, MA
3. Cambridge, MA
4. La Jolla, CA

Issue Date:

Tue Oct 04 00:00:00 EDT 2011

Research Org.:

The Scripps Research Inst., La Jolla, CA (United States)

Sponsoring Org.:

USDOE

OSTI Identifier:

1029285

Patent Number(s):

8030028

Application Number:

12/590,742

Assignee:

The Scripps Research Institute (La Jolla, CA)

Patent Classifications (CPCs):

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {

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C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

C12N15/70 - Vectors or expression systems specially adapted for E. coli
C12N9/93 - {Ligases

C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {

C12P21/00 - Preparation of peptides or proteins
C12P21/02 - having a known sequence of two or more amino acids, e.g. glutathione

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DOE Contract Number:  

FG03-00ER45812

Resource Type:

Patent

Country of Publication:

United States

Language:

English

Subject:

59 BASIC BIOLOGICAL SCIENCES