skip to main content
DOE Patents title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Compositions of orthogonal lysyl-tRNA and aminoacyl-tRNA synthetase pairs and uses thereof

Abstract

Compositions and methods of producing components of protein biosynthetic machinery that include orthogonal lysyl-tRNAs, orthogonal lysyl-aminoacyl-tRNA synthetases, and orthogonal pairs of lysyl-tRNAs/synthetases, which incorporate homoglutamines into proteins are provided in response to a four base codon. Methods for identifying these orthogonal pairs are also provided along with methods of producing proteins with homoglutamines using these orthogonal pairs.

Inventors:
 [1];  [2];  [3];  [4]
  1. San Francisco, CA
  2. Brookline, MA
  3. Cambridge, MA
  4. La Jolla, CA
Issue Date:
Research Org.:
The Scripps Research Inst., La Jolla, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1029285
Patent Number(s):
8030028
Application Number:
12/590,742
Assignee:
The Scripps Research Institute (La Jolla, CA)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
DOE Contract Number:  
FG03-00ER45812
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Anderson, J Christopher, Wu, Ning, Santoro, Stephen, and Schultz, Peter G. Compositions of orthogonal lysyl-tRNA and aminoacyl-tRNA synthetase pairs and uses thereof. United States: N. p., 2011. Web.
Anderson, J Christopher, Wu, Ning, Santoro, Stephen, & Schultz, Peter G. Compositions of orthogonal lysyl-tRNA and aminoacyl-tRNA synthetase pairs and uses thereof. United States.
Anderson, J Christopher, Wu, Ning, Santoro, Stephen, and Schultz, Peter G. Tue . "Compositions of orthogonal lysyl-tRNA and aminoacyl-tRNA synthetase pairs and uses thereof". United States. https://www.osti.gov/servlets/purl/1029285.
@article{osti_1029285,
title = {Compositions of orthogonal lysyl-tRNA and aminoacyl-tRNA synthetase pairs and uses thereof},
author = {Anderson, J Christopher and Wu, Ning and Santoro, Stephen and Schultz, Peter G},
abstractNote = {Compositions and methods of producing components of protein biosynthetic machinery that include orthogonal lysyl-tRNAs, orthogonal lysyl-aminoacyl-tRNA synthetases, and orthogonal pairs of lysyl-tRNAs/synthetases, which incorporate homoglutamines into proteins are provided in response to a four base codon. Methods for identifying these orthogonal pairs are also provided along with methods of producing proteins with homoglutamines using these orthogonal pairs.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2011},
month = {10}
}

Patent:

Save / Share:

Works referenced in this record:

Four-base codons ACCA, ACCU and ACCC are recognized by frameshift suppressor sufJ
journal, August 1981


Exploring the Limits of Codon and Anticodon Size
journal, February 2002


Structural basis for orthogonal tRNA specificities of tyrosyl-tRNA synthetases for genetic code expansion
journal, May 2003


Incorporation of Nonnatural Amino Acids into Streptavidin through In Vitro Frame-Shift Suppression
journal, January 1996


Substrate recognition by class I lysyl-tRNA synthetases: A molecular basis for gene displacement
journal, January 1999


Actions of the anticodon arm in translation on the phenotypes of RNA mutants
journal, November 1986


An expanded genetic code with a functional quadruplet codon
journal, May 2004


Generation of a Bacterium with a 21 Amino Acid Genetic Code
journal, January 2003

  • Mehl, Ryan A.; Anderson, J. Christopher; Santoro, Stephen W.
  • Journal of the American Chemical Society, Vol. 125, Issue 4, p. 935-939
  • https://doi.org/10.1021/ja0284153

An Expanded Eukaryotic Genetic Code
journal, August 2003


Adaptation of an Orthogonal Archaeal Leucyl-tRNA and Synthetase Pair for Four-base, Amber, and Opal Suppression
journal, August 2003


Addition of a photocrosslinking amino acid to the genetic code of Escherichia coli
journal, August 2002


Introduction of unnatural amino acids into chalcone isomerase
journal, July 1991


Expanding the genetic code: selection of efficient suppressors of four-base codons and identification of “shifty” four-base codons with a library approach in Escherichia coli
journal, March 2001


An efficient system for the evolution of aminoacyl-tRNA synthetase specificity
journal, September 2002


Functional Annotation of Class I Lysyl-tRNA Synthetase Phylogeny Indicates a Limited Role for Gene Transfer
journal, August 2002


Incorporation of non-natural amino acids into proteins
journal, December 2002


A New Functional Suppressor tRNA/Aminoacyl−tRNA Synthetase Pair for the in Vivo Incorporation of Unnatural Amino Acids into Proteins
journal, May 2000


Incorporation of two nonnatural amino acids into proteins through extension of the genetic code
journal, November 1999


Functional convergence of two lysyl-tRNA synthetases with unrelated topologies
journal, March 2002


An expanding genetic code
journal, December 2004


Adding new meanings to the genetic code
journal, June 2001


Five-base codons for incorporation of nonnatural amino acids into proteins
journal, September 2001


Addition of the keto functional group to the genetic code of Escherichia coli
journal, December 2002


Expanding the Genetic Code of Escherichia coli
journal, April 2001


Reading frame selection and transfer RNA anticodon loop stacking
journal, December 1987


Novel transfer RNAs that are active in Escherichia coli
journal, May 1992