Pressure cryocooling protein crystals
Abstract
Preparation of cryocooled protein crystal is provided by use of helium pressurizing and cryocooling to obtain cryocooled protein crystal allowing collection of high resolution data and by heavier noble gas (krypton or xenon) binding followed by helium pressurizing and cryocooling to obtain cryocooled protein crystal for collection of high resolution data and SAD phasing simultaneously. The helium pressurizing is carried out on crystal coated to prevent dehydration or on crystal grown in aqueous solution in a capillary.
- Inventors:
-
- Ithaca, NY
- Issue Date:
- Research Org.:
- Cornell Univ., Ithaca, NY (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1029280
- Patent Number(s):
- 8030449
- Application Number:
- 11/700,237
- Assignee:
- Cornell Research Foundation, Inc. (Ithaca, NY)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C30 - CRYSTAL GROWTH C30B - SINGLE-CRYSTAL-GROWTH
- DOE Contract Number:
- FG02-97ER62443
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 36 MATERIALS SCIENCE
Citation Formats
Kim, Chae Un, and Gruner, Sol M. Pressure cryocooling protein crystals. United States: N. p., 2011.
Web.
Kim, Chae Un, & Gruner, Sol M. Pressure cryocooling protein crystals. United States.
Kim, Chae Un, and Gruner, Sol M. Tue .
"Pressure cryocooling protein crystals". United States. https://www.osti.gov/servlets/purl/1029280.
@article{osti_1029280,
title = {Pressure cryocooling protein crystals},
author = {Kim, Chae Un and Gruner, Sol M},
abstractNote = {Preparation of cryocooled protein crystal is provided by use of helium pressurizing and cryocooling to obtain cryocooled protein crystal allowing collection of high resolution data and by heavier noble gas (krypton or xenon) binding followed by helium pressurizing and cryocooling to obtain cryocooled protein crystal for collection of high resolution data and SAD phasing simultaneously. The helium pressurizing is carried out on crystal coated to prevent dehydration or on crystal grown in aqueous solution in a capillary.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2011},
month = {10}
}
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