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Title: High expression Zymomonas promoters

Abstract

Identified are mutants of the promoter of the Z. mobilis glyceraldehyde-3-phosphate dehydrogenase gene, which direct improved expression levels of operably linked heterologous nucleic acids. These are high expression promoters useful for expression of chimeric genes in Zymomonas, Zymobacter, and other related bacteria.

Inventors:
 [1];  [2];  [3];  [4];  [5];  [6];  [7];  [8]
  1. West Chester, PA
  2. Havertown, PA
  3. New Hope, PA
  4. Kennett Square, PA
  5. (East Fallowfield, PA): Zhang, Min (Lakewood, CO)
  6. Lakewood, CO
  7. Wilmington, DE
  8. Centennial, CO
Issue Date:
Research Org.:
E.I. du Pont de Nemours and Company Alliance for Sustainable Energy LLC (Wilmington, DE)
Sponsoring Org.:
USDOE
OSTI Identifier:
1026671
Patent Number(s):
7989206
Application Number:
12/410,495
Assignee:
E.I. du Pont de Nemours and Company Alliance for Sustainable Energy LLC (Wilmington, DE)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
DOE Contract Number:  
FC36-03GO13146; AC36-99GO10337
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Viitanen, Paul V, Tao, Luan, Zhang, Yuying, Caimi, Perry G, McCole, Laura, Chou, Yat-Chen, McCutchen, Carol M, and Franden, Mary Ann. High expression Zymomonas promoters. United States: N. p., 2011. Web.
Viitanen, Paul V, Tao, Luan, Zhang, Yuying, Caimi, Perry G, McCole, Laura, Chou, Yat-Chen, McCutchen, Carol M, & Franden, Mary Ann. High expression Zymomonas promoters. United States.
Viitanen, Paul V, Tao, Luan, Zhang, Yuying, Caimi, Perry G, McCole, Laura, Chou, Yat-Chen, McCutchen, Carol M, and Franden, Mary Ann. Tue . "High expression Zymomonas promoters". United States. https://www.osti.gov/servlets/purl/1026671.
@article{osti_1026671,
title = {High expression Zymomonas promoters},
author = {Viitanen, Paul V and Tao, Luan and Zhang, Yuying and Caimi, Perry G and McCole, Laura and Chou, Yat-Chen and McCutchen, Carol M and Franden, Mary Ann},
abstractNote = {Identified are mutants of the promoter of the Z. mobilis glyceraldehyde-3-phosphate dehydrogenase gene, which direct improved expression levels of operably linked heterologous nucleic acids. These are high expression promoters useful for expression of chimeric genes in Zymomonas, Zymobacter, and other related bacteria.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2011},
month = {8}
}

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Works referenced in this record:

Genetic Engineering of Zymobacter palmae for Production of Ethanol from Xylose
journal, February 2007


Pentose metabolism in Zymomonas mobilis wild-type and recombinant strains
journal, December 1992


Metabolic Engineering of a Pentose Metabolism Pathway in Ethanologenic Zymomonas mobilis
journal, January 1995


Sequences of versatile broad-host-range vectors of the RK2 family
journal, July 2003


Promoter selectivity of Escherichia coli RNA polymerase: effect of base substitutions in the promoter −35 region on promoter strength
journal, January 1990


An efficient system for markerless gene replacement applicable in a wide variety of enterobacterial species
journal, January 2007


Chromosomal promoter replacement of the isoprenoid pathway for enhancing carotenoid production in E. coli
journal, January 2006


Metabolic redesign of vitamin E biosynthesis in plants for tocotrienol production and increased antioxidant content
journal, August 2003