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Title: Zymomonas with improved xylose utilization

Abstract

Strains of Zymomonas were engineered by introducing a chimeric xylose isomerase gene that contains a mutant promoter of the Z. mobilis glyceraldehyde-3-phosphate dehydrogenase gene. The promoter directs increased expression of xylose isomerase, and when the strain is in addition engineered for expression of xylulokinase, transaldolase and transketolase, improved utilization of xylose is obtained.

Inventors:
 [1];  [2];  [3];  [4];  [5];  [6];  [7];  [7];  [8]
  1. West Chester, PA
  2. Havertown, PA
  3. New Hope, PA
  4. Kennett Square, PA
  5. Wilmington, DE
  6. East Fallowfield, PA
  7. Lakewood, CO
  8. Centennial, CO
Issue Date:
Research Org.:
E. I. du Pont de Nemours and Company (Wilmington, DE)
Sponsoring Org.:
USDOE
OSTI Identifier:
1025535
Patent Number(s):
7998722
Application Number:
12/410,501
Assignee:
E. I. du Pont de Nemours and Company (Wilmington, DE); Alliance for Sustainable Energy LLC (Wilmington, DE)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
DOE Contract Number:  
FC36-03GO13146
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Viitanen, Paul V, Tao, Luan, Zhang, Yuying, Caimi, Perry G, McCutchen, Carol M, McCole, Laura, Zhang, Min, Chou, Yat-Chen, and Franden, Mary Ann. Zymomonas with improved xylose utilization. United States: N. p., 2011. Web.
Viitanen, Paul V, Tao, Luan, Zhang, Yuying, Caimi, Perry G, McCutchen, Carol M, McCole, Laura, Zhang, Min, Chou, Yat-Chen, & Franden, Mary Ann. Zymomonas with improved xylose utilization. United States.
Viitanen, Paul V, Tao, Luan, Zhang, Yuying, Caimi, Perry G, McCutchen, Carol M, McCole, Laura, Zhang, Min, Chou, Yat-Chen, and Franden, Mary Ann. Tue . "Zymomonas with improved xylose utilization". United States. https://www.osti.gov/servlets/purl/1025535.
@article{osti_1025535,
title = {Zymomonas with improved xylose utilization},
author = {Viitanen, Paul V and Tao, Luan and Zhang, Yuying and Caimi, Perry G and McCutchen, Carol M and McCole, Laura and Zhang, Min and Chou, Yat-Chen and Franden, Mary Ann},
abstractNote = {Strains of Zymomonas were engineered by introducing a chimeric xylose isomerase gene that contains a mutant promoter of the Z. mobilis glyceraldehyde-3-phosphate dehydrogenase gene. The promoter directs increased expression of xylose isomerase, and when the strain is in addition engineered for expression of xylulokinase, transaldolase and transketolase, improved utilization of xylose is obtained.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2011},
month = {8}
}

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