Title: High speed nucleic acid sequencing

Abstract

The present invention is directed to a method of sequencing a target nucleic acid molecule having a plurality of bases. In its principle, the temporal order of base additions during the polymerization reaction is measured on a molecule of nucleic acid. Each type of labeled nucleotide comprises an acceptor fluorophore attached to a phosphate portion of the nucleotide such that the fluorophore is removed upon incorporation into a growing strand. Fluorescent signal is emitted via fluorescent resonance energy transfer between the donor fluorophore and the acceptor fluorophore as each nucleotide is incorporated into the growing strand. The sequence is deduced by identifying which base is being incorporated into the growing strand.

Inventors:

Korlach, Jonas ^[1]; Webb, Watt W ^[1]; Levene, Michael ^[1]; Turner, Stephen ^[1]; Craighead, Harold G ^[1]; Foquet, Mathieu ^[1]

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+ Show Inventor Affiliations

1. Ithaca, NY

Issue Date:

Tue May 17 00:00:00 EDT 2011

Research Org.:

Cornell Research Foundation (Ithaca, NY)

Sponsoring Org.:

USDOE

OSTI Identifier:

1020256

Patent Number(s):

7943305

Application Number:

11/279,711

Assignee:

Cornell Research Foundation (Ithaca, NY)

Patent Classifications (CPCs):

C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS

Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y10 - TECHNICAL SUBJECTS COVERED BY FORMER USPC Y10S - TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS

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C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS

C12Q1/6869 - Methods for sequencing
C12Q1/6874 - involving nucleic acid arrays, e.g. sequencing by hybridisation
C12Q2521/543 - Immobilised enzyme
C12Q2537/149 - Sequential reactions
C12Q2561/113 - Real time assay
C12Q2561/12 - Fluorescence lifetime measurement
C12Q2565/518 - characterised by the immobilisation of the nucleic acid sample or target
C12Q2565/537 - characterised by the capture oligonucleotide acting as a primer

Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y10 - TECHNICAL SUBJECTS COVERED BY FORMER USPC Y10S - TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS

Y10S436/80 - Fluorescent dyes, e.g. rhodamine
Y10S436/805 - Optical property

Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y10 - TECHNICAL SUBJECTS COVERED BY FORMER USPC Y10T - TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION

Y10T436/143333 - Saccharide [e.g., DNA, etc.]

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DOE Contract Number:  

066898-0003891

Resource Type:

Patent

Country of Publication:

United States

Language:

English

Subject:

59 BASIC BIOLOGICAL SCIENCES