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Title: High speed nucleic acid sequencing

Abstract

The present invention is directed to a method of sequencing a target nucleic acid molecule having a plurality of bases. In its principle, the temporal order of base additions during the polymerization reaction is measured on a molecule of nucleic acid. Each type of labeled nucleotide comprises an acceptor fluorophore attached to a phosphate portion of the nucleotide such that the fluorophore is removed upon incorporation into a growing strand. Fluorescent signal is emitted via fluorescent resonance energy transfer between the donor fluorophore and the acceptor fluorophore as each nucleotide is incorporated into the growing strand. The sequence is deduced by identifying which base is being incorporated into the growing strand.

Inventors:
 [1];  [1];  [1];  [1];  [1];  [1]
  1. Ithaca, NY
Issue Date:
Research Org.:
Cornell Research Foundation (Ithaca, NY)
Sponsoring Org.:
USDOE
OSTI Identifier:
1020256
Patent Number(s):
7943305
Application Number:
11/279,711
Assignee:
Cornell Research Foundation (Ithaca, NY)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y10 - TECHNICAL SUBJECTS COVERED BY FORMER USPC Y10S - TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
DOE Contract Number:  
066898-0003891
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Korlach, Jonas, Webb, Watt W, Levene, Michael, Turner, Stephen, Craighead, Harold G, and Foquet, Mathieu. High speed nucleic acid sequencing. United States: N. p., 2011. Web.
Korlach, Jonas, Webb, Watt W, Levene, Michael, Turner, Stephen, Craighead, Harold G, & Foquet, Mathieu. High speed nucleic acid sequencing. United States.
Korlach, Jonas, Webb, Watt W, Levene, Michael, Turner, Stephen, Craighead, Harold G, and Foquet, Mathieu. Tue . "High speed nucleic acid sequencing". United States. https://www.osti.gov/servlets/purl/1020256.
@article{osti_1020256,
title = {High speed nucleic acid sequencing},
author = {Korlach, Jonas and Webb, Watt W and Levene, Michael and Turner, Stephen and Craighead, Harold G and Foquet, Mathieu},
abstractNote = {The present invention is directed to a method of sequencing a target nucleic acid molecule having a plurality of bases. In its principle, the temporal order of base additions during the polymerization reaction is measured on a molecule of nucleic acid. Each type of labeled nucleotide comprises an acceptor fluorophore attached to a phosphate portion of the nucleotide such that the fluorophore is removed upon incorporation into a growing strand. Fluorescent signal is emitted via fluorescent resonance energy transfer between the donor fluorophore and the acceptor fluorophore as each nucleotide is incorporated into the growing strand. The sequence is deduced by identifying which base is being incorporated into the growing strand.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2011},
month = {5}
}

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Works referenced in this record:

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