Flow cytometric detection method for DNA samples
Abstract
Disclosed herein are two methods for rapid multiplex analysis to determine the presence and identity of target DNA sequences within a DNA sample. Both methods use reporting DNA sequences, e.g., modified conventional Taqman.RTM. probes, to combine multiplex PCR amplification with microsphere-based hybridization using flow cytometry means of detection. Real-time PCR detection can also be incorporated. The first method uses a cyanine dye, such as, Cy3.TM., as the reporter linked to the 5' end of a reporting DNA sequence. The second method positions a reporter dye, e.g., FAM.TM. on the 3' end of the reporting DNA sequence and a quencher dye, e.g., TAMRA.TM., on the 5' end.
- Inventors:
-
- Livermore, CA
- Round Rock, TX
- Issue Date:
- Research Org.:
- Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1019570
- Patent Number(s):
- 7972818
- Application Number:
- US Patent Application 11/454,478
- Assignee:
- Lawrence Livermore National Security, LLC (Livermore, CA)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
- DOE Contract Number:
- W-7405-ENG-48
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Nasarabadi, Shanavaz, Langlois, Richard G, and Venkateswaran, Kodumudi S. Flow cytometric detection method for DNA samples. United States: N. p., 2011.
Web.
Nasarabadi, Shanavaz, Langlois, Richard G, & Venkateswaran, Kodumudi S. Flow cytometric detection method for DNA samples. United States.
Nasarabadi, Shanavaz, Langlois, Richard G, and Venkateswaran, Kodumudi S. Tue .
"Flow cytometric detection method for DNA samples". United States. https://www.osti.gov/servlets/purl/1019570.
@article{osti_1019570,
title = {Flow cytometric detection method for DNA samples},
author = {Nasarabadi, Shanavaz and Langlois, Richard G and Venkateswaran, Kodumudi S},
abstractNote = {Disclosed herein are two methods for rapid multiplex analysis to determine the presence and identity of target DNA sequences within a DNA sample. Both methods use reporting DNA sequences, e.g., modified conventional Taqman.RTM. probes, to combine multiplex PCR amplification with microsphere-based hybridization using flow cytometry means of detection. Real-time PCR detection can also be incorporated. The first method uses a cyanine dye, such as, Cy3.TM., as the reporter linked to the 5' end of a reporting DNA sequence. The second method positions a reporter dye, e.g., FAM.TM. on the 3' end of the reporting DNA sequence and a quencher dye, e.g., TAMRA.TM., on the 5' end.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2011},
month = {7}
}
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