Genomic library construction

Church, George M; Zhang, Kun

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Title: Genomic library construction

Abstract

Compositions and methods for amplifying nucleic acid sequences from a single cell are provided. Compositions and methods for constructing a genomic library from a single cell are also provided.

Inventors:

Church, George M ^[1]; Zhang, Kun ^[2]

Brookline, MA
San Diego, CA

Issue Date:: Tue Jul 26 00:00:00 EDT 2011

Research Org.:: Harvard University

Sponsoring Org.:: USDOE

OSTI Identifier:: 1019402

Patent Number(s):: 7985546

Application Number:: US Patent Application 12/265,184

Assignee:: President and Fellows of Harvard College (Cambridge, MA)

Patent Classifications (CPCs):: C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS

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C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C12N15/1093 - {General methods of preparing gene libraries, not provided for in other subgroups}

C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
C12Q1/686 - Polymerase chain reaction [PCR]
C12Q2521/101 - DNA polymerase
C12Q2521/307 - Single strand endonuclease

Show less

DOE Contract Number:: FG02-02ER63445

Resource Type:: Patent

Country of Publication:: United States

Language:: English

Subject:: 47 OTHER INSTRUMENTATION

Citation Formats


                    Church, George M, and Zhang, Kun. Genomic library construction.  United States: N. p., 2011. 
        Web.

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                    Church, George M, & Zhang, Kun. Genomic library construction.  United States.

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                    Church, George M, and Zhang, Kun. Tue .  
        "Genomic library construction".  United States.  https://www.osti.gov/servlets/purl/1019402.

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@article{osti_1019402,

  title        = {Genomic library construction},

  author       = {Church, George M and Zhang, Kun},

  abstractNote = {Compositions and methods for amplifying nucleic acid sequences from a single cell are provided. Compositions and methods for constructing a genomic library from a single cell are also provided.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {Tue Jul 26 00:00:00 EDT 2011},

  month        = {Tue Jul 26 00:00:00 EDT 2011}

}

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Works referenced in this record:

Mutation detection and single-molecule counting using isothermal rolling-circleamplification
journal, July 1998

Lizardi, Paul M.; Huang, Xiaohua; Zhu, Zhengrong
Nature Genetics, Vol. 19, Issue 3, p. 225-232
https://doi.org/10.1038/898

Genomic DNA Amplification from a Single Bacterium
journal, June 2005

Raghunathan, A.; Ferguson, H. R.; Bornarth, C. J.
Applied and Environmental Microbiology, Vol. 71, Issue 6
https://doi.org/10.1128/AEM.71.6.3342-3347.2005

Whole Genome Analysis of Genetic Alterations in Small DNA Samples Using Hyperbranched Strand Displacement Amplification and Array-CGH
journal, February 2003

Lage, J. M.
Genome Research, Vol. 13, Issue 2
https://doi.org/10.1101/gr.377203

Isothermal Strand-Displacement Amplification Applications for High-Throughput Genomics
journal, December 2002

Detter, John C.; Jett, Jamie M.; Lucas, Susan M.
Genomics, Vol. 80, Issue 6
https://doi.org/10.1006/geno.2002.7020

Ligation overcomes terminal underrepresentation in multiple displacement amplification of linear DNA
journal, August 2005

Panelli, Simona; Damiani, Giuseppe; Espen, Luca
BioTechniques, Vol. 39, Issue 2
https://doi.org/10.2144/05392BM03

Genome coverage and sequence fidelity of 29 polymerase-based multiple strand displacement whole genome amplification
journal, May 2004

Paez, J. G.
Nucleic Acids Research, Vol. 32, Issue 9
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Fine-scale phylogenetic architecture of a complex bacterial community
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Acinas, Silvia G.; Klepac-Ceraj, Vanja; Hunt, Dana E.
Nature, Vol. 430, Issue 6999
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Advanced sequencing technologies: methods and goals
journal, May 2004

Shendure, Jay; Mitra, Robi D.; Varma, Chris
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Comprehensive human genome amplification using multiple displacement amplification
journal, April 2002

Dean, F. B.; Hosono, S.; Fang, L.
Proceedings of the National Academy of Sciences, Vol. 99, Issue 8, p. 5261-5266
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Whole genome amplification from a single cell: implications for genetic analysis.
journal, July 1992

Zhang, L.; Cui, X.; Schmitt, K.
Proceedings of the National Academy of Sciences, Vol. 89, Issue 13
https://doi.org/10.1073/pnas.89.13.5847

Degenerate oligonucleotide-primed PCR: General amplification of target DNA by a single degenerate primer
journal, July 1992

Telenius, Ha˚kan; Carter, Nigel P.; Bebb, Charlotte E.
Genomics, Vol. 13, Issue 3
https://doi.org/10.1016/0888-7543(92)90147-K

Isothermal Amplification and Multimerization of DNA by Bst DNA Polymerase
journal, April 2001

Hafner, G. J.; Yang, I. C.; Wolter, L. C.
BioTechniques, Vol. 30, Issue 4
https://doi.org/10.2144/01304rr03

Cell-free cloning using φ29 DNA polymerase
journal, November 2005

Hutchison, C. A.; Smith, H. O.; Pfannkoch, C.
Proceedings of the National Academy of Sciences, Vol. 102, Issue 48, p. 17332-17336
https://doi.org/10.1073/pnas.0508809102

Unbiased Whole-Genome Amplification Directly From Clinical Samples
journal, April 2003

Hosono, S.
Genome Research, Vol. 13, Issue 5
https://doi.org/10.1101/gr.816903

Accurate Multiplex Polony Sequencing of an Evolved Bacterial Genome
journal, September 2005

Shendure, J.
Science, Vol. 309, Issue 5741
https://doi.org/10.1126/science.1117389

Genotypic Diversity Within a Natural Coastal Bacterioplankton Population
journal, February 2005

Thompson, J. R.
Science, Vol. 307, Issue 5713
https://doi.org/10.1126/science.1106028

Isothermal in vitro amplification of DNA by a restriction enzyme/DNA polymerase system.
journal, January 1992

Walker, G. T.; Little, M. C.; Nadeau, J. G.
Proceedings of the National Academy of Sciences, Vol. 89, Issue 1, p. 392-396
https://doi.org/10.1073/pnas.89.1.392

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Similar Records

Title: Genomic library construction

Abstract

Citation Formats

Mutation detection and single-molecule counting using isothermal rolling-circleamplification journal, July 1998

Genomic DNA Amplification from a Single Bacterium journal, June 2005

Whole Genome Analysis of Genetic Alterations in Small DNA Samples Using Hyperbranched Strand Displacement Amplification and Array-CGH journal, February 2003

Isothermal Strand-Displacement Amplification Applications for High-Throughput Genomics journal, December 2002

Ligation overcomes terminal underrepresentation in multiple displacement amplification of linear DNA journal, August 2005

Genome coverage and sequence fidelity of 29 polymerase-based multiple strand displacement whole genome amplification journal, May 2004

Fine-scale phylogenetic architecture of a complex bacterial community journal, July 2004

Advanced sequencing technologies: methods and goals journal, May 2004

Comprehensive human genome amplification using multiple displacement amplification journal, April 2002

Whole genome amplification from a single cell: implications for genetic analysis. journal, July 1992

Degenerate oligonucleotide-primed PCR: General amplification of target DNA by a single degenerate primer journal, July 1992

Isothermal Amplification and Multimerization of DNA by Bst DNA Polymerase journal, April 2001

Cell-free cloning using φ29 DNA polymerase journal, November 2005

Unbiased Whole-Genome Amplification Directly From Clinical Samples journal, April 2003

Accurate Multiplex Polony Sequencing of an Evolved Bacterial Genome journal, September 2005

Genotypic Diversity Within a Natural Coastal Bacterioplankton Population journal, February 2005

Isothermal in vitro amplification of DNA by a restriction enzyme/DNA polymerase system. journal, January 1992

Mutation detection and single-molecule counting using isothermal rolling-circleamplification
journal, July 1998

Genomic DNA Amplification from a Single Bacterium
journal, June 2005

Whole Genome Analysis of Genetic Alterations in Small DNA Samples Using Hyperbranched Strand Displacement Amplification and Array-CGH
journal, February 2003

Isothermal Strand-Displacement Amplification Applications for High-Throughput Genomics
journal, December 2002

Ligation overcomes terminal underrepresentation in multiple displacement amplification of linear DNA
journal, August 2005

Genome coverage and sequence fidelity of 29 polymerase-based multiple strand displacement whole genome amplification
journal, May 2004

Fine-scale phylogenetic architecture of a complex bacterial community
journal, July 2004

Advanced sequencing technologies: methods and goals
journal, May 2004

Comprehensive human genome amplification using multiple displacement amplification
journal, April 2002

Whole genome amplification from a single cell: implications for genetic analysis.
journal, July 1992

Degenerate oligonucleotide-primed PCR: General amplification of target DNA by a single degenerate primer
journal, July 1992

Isothermal Amplification and Multimerization of DNA by Bst DNA Polymerase
journal, April 2001

Cell-free cloning using φ29 DNA polymerase
journal, November 2005

Unbiased Whole-Genome Amplification Directly From Clinical Samples
journal, April 2003

Accurate Multiplex Polony Sequencing of an Evolved Bacterial Genome
journal, September 2005

Genotypic Diversity Within a Natural Coastal Bacterioplankton Population
journal, February 2005

Isothermal in vitro amplification of DNA by a restriction enzyme/DNA polymerase system.
journal, January 1992