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Title: Nucleic acid encoding a self-assembling split-fluorescent protein system

Abstract

The invention provides a protein labeling and detection system based on self-complementing fragments of fluorescent and chromophoric proteins. The system of the invention is exemplified with various combinations of self-complementing fragments derived from Aequorea victoria Green Fluorescent Protein (GFP), which are used to detect and quantify protein solubility in multiple assay formats, both in vitro and in vivo.

Inventors:
 [1];  [2]
  1. Santa Fe, NM
  2. Los Alamos, NM
Issue Date:
Research Org.:
Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1019114
Patent Number(s):
7955821
Application Number:
US Patent Application 10/973,693
Assignee:
Los Alamos National Security, LLC (Los Alamos, NM)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES
DOE Contract Number:  
FG02-98ER62647; W-7405-ENG-36
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Waldo, Geoffrey S, and Cabantous, Stephanie. Nucleic acid encoding a self-assembling split-fluorescent protein system. United States: N. p., 2011. Web.
Waldo, Geoffrey S, & Cabantous, Stephanie. Nucleic acid encoding a self-assembling split-fluorescent protein system. United States.
Waldo, Geoffrey S, and Cabantous, Stephanie. Tue . "Nucleic acid encoding a self-assembling split-fluorescent protein system". United States. https://www.osti.gov/servlets/purl/1019114.
@article{osti_1019114,
title = {Nucleic acid encoding a self-assembling split-fluorescent protein system},
author = {Waldo, Geoffrey S and Cabantous, Stephanie},
abstractNote = {The invention provides a protein labeling and detection system based on self-complementing fragments of fluorescent and chromophoric proteins. The system of the invention is exemplified with various combinations of self-complementing fragments derived from Aequorea victoria Green Fluorescent Protein (GFP), which are used to detect and quantify protein solubility in multiple assay formats, both in vitro and in vivo.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2011},
month = {6}
}

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