DOE Patents title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Methods of using thermal tolerant avicelase from Acidothermus cellulolyticus

Abstract

The invention provides a thermal tolerant (thermostable) cellulase, AviIII, that is a member of the glycoside hydrolase (GH) family. AviIII was isolated and characterized from Acidothermus cellulolyticus, and, like many cellulases, the disclosed polypeptide and/or its derivatives may be useful for the conversion of biomass into biofuels and chemicals.

Inventors:
 [1];  [1];  [1];  [1]
  1. Golden, CO
Issue Date:
Research Org.:
National Renewable Energy Laboratory (NREL), Golden, CO (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1016674
Patent Number(s):
7932054
Application Number:
US Patent Application 12/425,299
Assignee:
Alliance for Sustainable Energy, LLC (Golden, CO)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C - CHEMISTRY C12 - BIOCHEMISTRY C12Y - ENZYMES
DOE Contract Number:  
AC36-08GO28308
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Adney, William S, Vinzant, Todd B, Ding, Shih-You, and Himmel, Michael E. Methods of using thermal tolerant avicelase from Acidothermus cellulolyticus. United States: N. p., 2011. Web.
Adney, William S, Vinzant, Todd B, Ding, Shih-You, & Himmel, Michael E. Methods of using thermal tolerant avicelase from Acidothermus cellulolyticus. United States.
Adney, William S, Vinzant, Todd B, Ding, Shih-You, and Himmel, Michael E. Tue . "Methods of using thermal tolerant avicelase from Acidothermus cellulolyticus". United States. https://www.osti.gov/servlets/purl/1016674.
@article{osti_1016674,
title = {Methods of using thermal tolerant avicelase from Acidothermus cellulolyticus},
author = {Adney, William S and Vinzant, Todd B and Ding, Shih-You and Himmel, Michael E},
abstractNote = {The invention provides a thermal tolerant (thermostable) cellulase, AviIII, that is a member of the glycoside hydrolase (GH) family. AviIII was isolated and characterized from Acidothermus cellulolyticus, and, like many cellulases, the disclosed polypeptide and/or its derivatives may be useful for the conversion of biomass into biofuels and chemicals.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2011},
month = {4}
}

Works referenced in this record:

Production of glucose by enzymatic hydrolysis of cellulose.
journal, January 1968


celA from Bacillus lautus PL236 encodes a novel cellulose-binding endo-beta-1,4-glucanase.
journal, January 1992


Oligodeoxynucleotide-directed mutagenesis using the yeast transformation system
journal, January 1986


Genetics of somatic mammalian cells, VII. Induction and isolation of nutritional mutants in Chinese hamster cells.
journal, August 1968


Expression of prokaryotic genes for hygromycin B and G418 resistance as dominant-selection markers in mouse L cells
journal, October 1984


Prediction of protein function from protein sequence and structure
journal, January 1999


Multidomain and Multifunctional Glycosyl Hydrolases from the Extreme Thermophile Caldicellulosiruptor Isolate Tok7B.1
journal, May 2000


Manipulation of Adenovirus Vectors
book, January 1991


Computational protein engineering
journal, July 1998


Updating the sequence-based classification of glycosyl hydrolases
journal, June 1996


Trends in the Development of Baculovirus Expression Vectors
journal, January 1988


ULBPs, Novel MHC Class I–Related Molecules, Bind to CMV Glycoprotein UL16 and Stimulate NK Cytotoxicity through the NKG2D Receptor
journal, February 2001


A genetic enrichment for mutations constructed by oligodeoxynucleotide-directed mutagenesis
journal, January 1985


Roles of the Catalytic Domain and Two Cellulose Binding Domains of Thermomonospora fusca E4 in Cellulose Hydrolysis
journal, January 1998


Selection for animal cells that express the Escherichia coli gene coding for xanthine-guanine phosphoribosyltransferase.
journal, April 1981


Transformation of mammalian cells with an amplifiable dominant-acting gene.
journal, June 1980


Endo-beta-1,4-glucanase gene of Bacillus subtilis DLG.
journal, January 1987


Column cellulose hydrolysis reactor: Cellulase adsorption profile
journal, December 1986


When blind is better: Protein design by evolution
journal, July 1998


Cellulose degrading enzymes and their potential industrial applications
journal, January 1997


Transformation of mouse fibroblasts to methotrexate resistance by a recombinant plasmid expressing a prokaryotic dihydrofolate reductase.
journal, March 1981


Nomenclature Committee of the International Union of Biochemistry andMolecular Biology (NC‐IUBMB)
journal, September 1999


The Mechanism of Enzymatic Cellulose Degradation. Purification and Some Properties of Two Different 1,4-beta-Glucan Glucanohydrolases from Trichoderma viride
journal, January 1976


A classification of glycosyl hydrolases based on amino acid sequence similarities
journal, December 1991


A new dominant hybrid selective marker for higher eukaryotic cells
journal, July 1981


Engineering an enzyme to resist boiling
journal, March 1998


Basic local alignment search tool
journal, October 1990


The leucine zipper: a hypothetical structure common to a new class of DNA binding proteins
journal, June 1988


Comparison of biosequences
journal, December 1981


Modified glycosylation of cellobiohydrolase I from a high cellulase-producing mutant strain of Trichoderma reesei
journal, August 1998


Ultra-Thermostable Cellulases From Acidothermus cellulolyticus: Comparison of Temperature Optima with Previously Reported Cellulases
journal, August 1989


Crystal structure of a bacterial family-III cellulose-binding domain: a general mechanism for attachment to cellulose.
journal, November 1996


Immunoaffinity Chromatographic Purification of Cellobiohydrolase II Mutants from RecombinantTrichoderma reeseiStrains Devoid of Major Endoglucanase Genes
journal, December 1996


DNA shuffling of a family of genes from diverse species accelerates directed evolution
journal, January 1998


Purification and properties of a novel type of exo-1,4-beta-glucanase (Avicelase II) from the cellulolytic thermophile Clostridium stercorarium
journal, September 1991


EglC, a New Endoglucanase from Aspergillus niger with Major Activity towards Xyloglucan
journal, April 2002


Lune/eye gone, a Pax-like protein, uses a partial paired domain and a homeodomain for DNA recognition
journal, November 1998


A new thermostable endoglucanase,Acidothermus cellulolyticus E1: Synergism withTrichoderma reesei CBH I and comparison to Thermomonospora fusca E5
journal, March 1994


Synergism Between Purified Bacterial and Fungal Cellulases
book, May 1996


Molecular evolution by staggered extension process (StEP) in vitro recombination
journal, March 1998


The Production of Cellulases
book, June 1969


Isolation and Characterization of Acidothermus cellulolyticus gen. nov., sp. nov., a New Genus of Thermophilic, Acidophilic, Cellulolytic Bacteria
journal, July 1986


Toward controlling gene expression at will: Selection and design of zinc finger domains recognizing each of the 5'-GNN-3' DNA target sequences
journal, March 1999


Cellulose-Binding Domains: Classification and Properties
book, May 1996


CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice
journal, January 1994


Deciphering the message in protein sequences: tolerance to amino acid substitutions
journal, March 1990


Protein tolerance to random amino acid change
journal, June 2004


Affinity Purification of Proteins Binding to GST Fusion Proteins
journal, January 1996


Cloning and transcription analysis of the Aspergillus aculeatus No. F-50 endoglucanase 2 (cmc2) gene
journal, January 2002