Variants of glycoside hydrolases
Abstract
The present invention relates to variants of a parent glycoside hydrolase, comprising a substitution at one or more positions corresponding to positions 21, 94, 157, 205, 206, 247, 337, 350, 373, 383, 438, 455, 467, and 486 of amino acids 1 to 513 of SEQ ID NO: 2, and optionally further comprising a substitution at one or more positions corresponding to positions 8, 22, 41, 49, 57, 113, 193, 196, 226, 227, 246, 251, 255, 259, 301, 356, 371, 411, and 462 of amino acids 1 to 513 of SEQ ID NO: 2 a substitution at one or more positions corresponding to positions 8, 22, 41, 49, 57, 113, 193, 196, 226, 227, 246, 251, 255, 259, 301, 356, 371, 411, and 462 of amino acids 1 to 513 of SEQ ID NO: 2, wherein the variants have glycoside hydrolase activity. The present invention also relates to nucleotide sequences encoding the variant glycoside hydrolases and to nucleic acid constructs, vectors, and host cells comprising the nucleotide sequences.
- Inventors:
-
- Davis, CA
- Hamilton, MT
- Carnation, WA
- Sacramento, CA
- Issue Date:
- Research Org.:
- Novozymes, Inc. (Davis, CA)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1016673
- Patent Number(s):
- 7932073
- Application Number:
- US Patent Application 11/891,249
- Assignee:
- Novozymes, Inc. (Davis, CA)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C11 - ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES C11D - DETERGENT COMPOSITIONS
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
- DOE Contract Number:
- AC36-98GO10337
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Teter, Sarah, Ward, Connie, Cherry, Joel, Jones, Aubrey, Harris, Paul, and Yi, Jung. Variants of glycoside hydrolases. United States: N. p., 2011.
Web.
Teter, Sarah, Ward, Connie, Cherry, Joel, Jones, Aubrey, Harris, Paul, & Yi, Jung. Variants of glycoside hydrolases. United States.
Teter, Sarah, Ward, Connie, Cherry, Joel, Jones, Aubrey, Harris, Paul, and Yi, Jung. Tue .
"Variants of glycoside hydrolases". United States. https://www.osti.gov/servlets/purl/1016673.
@article{osti_1016673,
title = {Variants of glycoside hydrolases},
author = {Teter, Sarah and Ward, Connie and Cherry, Joel and Jones, Aubrey and Harris, Paul and Yi, Jung},
abstractNote = {The present invention relates to variants of a parent glycoside hydrolase, comprising a substitution at one or more positions corresponding to positions 21, 94, 157, 205, 206, 247, 337, 350, 373, 383, 438, 455, 467, and 486 of amino acids 1 to 513 of SEQ ID NO: 2, and optionally further comprising a substitution at one or more positions corresponding to positions 8, 22, 41, 49, 57, 113, 193, 196, 226, 227, 246, 251, 255, 259, 301, 356, 371, 411, and 462 of amino acids 1 to 513 of SEQ ID NO: 2 a substitution at one or more positions corresponding to positions 8, 22, 41, 49, 57, 113, 193, 196, 226, 227, 246, 251, 255, 259, 301, 356, 371, 411, and 462 of amino acids 1 to 513 of SEQ ID NO: 2, wherein the variants have glycoside hydrolase activity. The present invention also relates to nucleotide sequences encoding the variant glycoside hydrolases and to nucleic acid constructs, vectors, and host cells comprising the nucleotide sequences.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2011},
month = {4}
}
Works referenced in this record:
Activity Studies and Crystal Structures of Catalytically Deficient Mutants of Cellobiohydrolase I fromTrichoderma reesei
journal, November 1996
- Ståhlberg, Jerry; Divne, Christina; Koivula, Anu
- Journal of Molecular Biology, Vol. 264, Issue 2
Engineering of a glycosidase Family 7 cellobiohydrolase to more alkaline pH optimum: the pH behaviour of Trichoderma reesei Cel7A and its E223S/ A224H/L225V/T226A/D262G mutant
journal, May 2001
- Becker, Dieter; Braet, Christophe; Brumer, Harry
- Biochemical Journal, Vol. 356, Issue 1, p. 19-30
The relationship between thermal stability and pH optimum studied with wild-type and mutant Trichoderma reesei cellobiohydrolase Cel7A: Stability of cellobiohydrolase Cel7A
journal, March 2003
- Boer, Harry; Koivula, Anu
- European Journal of Biochemistry, Vol. 270, Issue 5
Structural basis for enantiomer binding and separation of a common β-blocker: crystal structure of cellobiohydrolase Cel7A with bound (S)-propranolol at 1.9 Å resolution
journal, January 2001
- Ståhlberg, Jerry; Henriksson, Hongbin; Divne, Christina
- Journal of Molecular Biology, Vol. 305, Issue 1
Molecular cloning and sequence analysis of the cellobiohydrolase i gene fromTrichoderma koningii G-39
journal, January 1994
- Wey, Ta-Tung; Hseu, Tzong-Hsiung; Huang, Lina
- Current Microbiology, Vol. 28, Issue 1