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Title: Designer proton-channel transgenic algae for photobiological hydrogen production

Abstract

A designer proton-channel transgenic alga for photobiological hydrogen production that is specifically designed for production of molecular hydrogen (H.sub.2) through photosynthetic water splitting. The designer transgenic alga includes proton-conductive channels that are expressed to produce such uncoupler proteins in an amount sufficient to increase the algal H.sub.2 productivity. In one embodiment the designer proton-channel transgene is a nucleic acid construct (300) including a PCR forward primer (302), an externally inducible promoter (304), a transit targeting sequence (306), a designer proton-channel encoding sequence (308), a transcription and translation terminator (310), and a PCR reverse primer (312). In various embodiments, the designer proton-channel transgenic algae are used with a gas-separation system (500) and a gas-products-separation and utilization system (600) for photobiological H.sub.2 production.

Inventors:
 [1]
  1. Knoxville, TN
Issue Date:
Research Org.:
Lee, James Weifu (Knoxville, TN)
Sponsoring Org.:
USDOE
OSTI Identifier:
1016564
Patent Number(s):
7932437
Application Number:
US Patent Application 11/748,531
Assignee:
Lee, James Weifu (Knoxville, TN)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
DOE Contract Number:  
AC05-00OR22725
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES

Citation Formats

Lee, James Weifu. Designer proton-channel transgenic algae for photobiological hydrogen production. United States: N. p., 2011. Web.
Lee, James Weifu. Designer proton-channel transgenic algae for photobiological hydrogen production. United States.
Lee, James Weifu. Tue . "Designer proton-channel transgenic algae for photobiological hydrogen production". United States. https://www.osti.gov/servlets/purl/1016564.
@article{osti_1016564,
title = {Designer proton-channel transgenic algae for photobiological hydrogen production},
author = {Lee, James Weifu},
abstractNote = {A designer proton-channel transgenic alga for photobiological hydrogen production that is specifically designed for production of molecular hydrogen (H.sub.2) through photosynthetic water splitting. The designer transgenic alga includes proton-conductive channels that are expressed to produce such uncoupler proteins in an amount sufficient to increase the algal H.sub.2 productivity. In one embodiment the designer proton-channel transgene is a nucleic acid construct (300) including a PCR forward primer (302), an externally inducible promoter (304), a transit targeting sequence (306), a designer proton-channel encoding sequence (308), a transcription and translation terminator (310), and a PCR reverse primer (312). In various embodiments, the designer proton-channel transgenic algae are used with a gas-separation system (500) and a gas-products-separation and utilization system (600) for photobiological H.sub.2 production.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2011},
month = {4}
}

Works referenced in this record:

Oxygen Tolerance of the H 2 -sensing [NiFe] Hydrogenase from Ralstonia eutropha H16 Is Based on Limited Access of Oxygen to the Active Site
journal, April 2005


Identification of Lhcb Gene Family Encoding the Light-harvesting Chlorophyll-a/b Proteins of Photosystem II in Chlamydomonas reinhardtii
journal, August 2001


Temperature effect on production of hydrogen and oxygen byChlamydomonas cold strain CCMP1619 and wild-type 137c: Scientific note
journal, September 1995


Coordinate Copper- and Oxygen-responsive Cyc6 and Cpx1 Expression in Chlamydomonas Is Mediated by the Same Element
journal, February 2000


Microalgae: a green source of renewable H2
journal, December 2000


The role of carbon dioxide in light-activated hydrogen production by Chlamydomonas reinhardtii
journal, October 1993


Improvement of Photosynthetic CO2 Fixation at High Light Intensity Through Reduction of Chlorophyll Antenna Size
journal, January 2002