Concentration and separation of biological organisms by ultrafiltration and dielectrophoresis
Abstract
Disclosed is a method for monitoring sources of public water supply for a variety of pathogens by using a combination of ultrafiltration techniques together dielectrophoretic separation techniques. Because water-borne pathogens, whether present due to "natural" contamination or intentional introduction, would likely be present in drinking water at low concentrations when samples are collected for monitoring or outbreak investigations, an approach is needed to quickly and efficiently concentrate and separate particles such as viruses, bacteria, and parasites in large volumes of water (e.g., 100 L or more) while simultaneously reducing the sample volume to levels sufficient for detecting low concentrations of microbes (e.g., <10 mL). The technique is also designed to screen the separated microbes based on specific conductivity and size.
- Inventors:
-
- San Francisco, CA
- Decatur, GA
- Livermore, CA
- Issue Date:
- Research Org.:
- Sandia Corporation (Livermore, CA)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1016144
- Patent Number(s):
- 7811439
- Application Number:
- 11/499,371
- Assignee:
- Sandia Corporation (Livermore, CA)
- Patent Classifications (CPCs):
-
B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01D - SEPARATION
B - PERFORMING OPERATIONS B03 - SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS B03C - MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS
- DOE Contract Number:
- AC04-94AL85000
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
Citation Formats
Simmons, Blake A, Hill, Vincent R, Fintschenko, Yolanda, and Cummings, Eric B. Concentration and separation of biological organisms by ultrafiltration and dielectrophoresis. United States: N. p., 2010.
Web.
Simmons, Blake A, Hill, Vincent R, Fintschenko, Yolanda, & Cummings, Eric B. Concentration and separation of biological organisms by ultrafiltration and dielectrophoresis. United States.
Simmons, Blake A, Hill, Vincent R, Fintschenko, Yolanda, and Cummings, Eric B. Tue .
"Concentration and separation of biological organisms by ultrafiltration and dielectrophoresis". United States. https://www.osti.gov/servlets/purl/1016144.
@article{osti_1016144,
title = {Concentration and separation of biological organisms by ultrafiltration and dielectrophoresis},
author = {Simmons, Blake A and Hill, Vincent R and Fintschenko, Yolanda and Cummings, Eric B},
abstractNote = {Disclosed is a method for monitoring sources of public water supply for a variety of pathogens by using a combination of ultrafiltration techniques together dielectrophoretic separation techniques. Because water-borne pathogens, whether present due to "natural" contamination or intentional introduction, would likely be present in drinking water at low concentrations when samples are collected for monitoring or outbreak investigations, an approach is needed to quickly and efficiently concentrate and separate particles such as viruses, bacteria, and parasites in large volumes of water (e.g., 100 L or more) while simultaneously reducing the sample volume to levels sufficient for detecting low concentrations of microbes (e.g., <10 mL). The technique is also designed to screen the separated microbes based on specific conductivity and size.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2010},
month = {10}
}
Works referenced in this record:
Evaluation of a dielectrophoretic bacterial counting technique
journal, March 1999
- Brown, A. P.; Betts, W. B.; Harrison, A. B.
- Biosensors and Bioelectronics, Vol. 14, Issue 3
Development of a Rapid Method for Simultaneous Recovery of Diverse Microbes in Drinking Water by Ultrafiltration with Sodium Polyphosphate and Surfactants
journal, November 2005
- Hill, V. R.; Polaczyk, A. L.; Hahn, D.
- Applied and Environmental Microbiology, Vol. 71, Issue 11
Separation of viable and non-viable yeast using dielectrophoresis
journal, January 1994
- Markx, Gerard H.; Talary, Mark S.; Pethig, Ronald
- Journal of Biotechnology, Vol. 32, Issue 1
Dielectrophoretic manipulation and characterization of herpes simplex virus-1 capsids
journal, August 2001
- Hughes, Michael P.; Morgan, Hywel; Rixon, Frazer J.
- European Biophysics Journal, Vol. 30, Issue 4
Dose-dependent dielectrophoretic response of Cryptosporidium oocysts treated with ozone
journal, March 1996
- Quinn, C. M.; Archer, G. P.; Betts, W. B.
- Letters in Applied Microbiology, Vol. 22, Issue 3
Ultraviolet light inactivation of protozoa in drinking water: a Bayesian meta-analysis
journal, January 2004
- Qian, Song S.; Donnelly, Maureen; Schmelling, Daniel C.
- Water Research, Vol. 38, Issue 2
Sub-micron sized biological particle manipulation and characterisation
journal, May 2001
- Malyan, B.; Balachandran, W.
- Journal of Electrostatics, Vol. 51-52
Dielectrophoretic filter for separation and recovery of biological cells in water
journal, September 2003
- Suehiro, J.; Imamura, M.
- IEEE Transactions on Industry Applications, Vol. 39, Issue 5
A Comparison of Theoretical and Experimental Electrokinetic and Dielectrophoretic Flow Fields (Invited)
conference, June 2012
- Cummings, Eric
- 32nd AIAA Fluid Dynamics Conference and Exhibit
Dielectrophoretic trapping without embedded electrodes
conference, August 2000
- Cummings, Eric B.; Singh, Anup K.
- Micromachining and Microfabrication, SPIE Proceedings
Electrodeless dielectrophoresis for micro total analysis systems
journal, November 2003
- Chou, Chia-Fu; Zenhausern, F.
- IEEE Engineering in Medicine and Biology Magazine, Vol. 22, Issue 6, p. 62-67
Dielectrophoretic characterization and separation of micro-organisms
journal, March 1994
- Markx, G. H.; Huang, Y.; Zhou, X. F.
- Microbiology, Vol. 140, Issue 3
A Study of Living and Dead Yeast Cells Using Dielectrophoresis
journal, January 1968
- Crane, Joe S.; Pohl, Herbert A.
- Journal of The Electrochemical Society, Vol. 115, Issue 6
Electrodeless Dielectrophoresis of Single- and Double-Stranded DNA
journal, October 2002
- Chou, Chia-Fu; Tegenfeldt, Jonas O.; Bakajin, Olgica
- Biophysical Journal, Vol. 83, Issue 4
Dielectrophoretic separation of bacteria using a conductivity gradient
journal, November 1996
- Markx, Gerard H.; Dyda, Penelope A.; Pethig, Ronald
- Journal of Biotechnology, Vol. 51, Issue 2
Reducing the protein fouling of polysulfone surfaces and polysulfone ultrafiltration membranes: Optimization of the type of presorbed layer
journal, August 1990
- Brink, L. E. S.; Romijn, D. J.
- Desalination, Vol. 78, Issue 2
The Motion and Precipitation of Suspensoids in Divergent Electric Fields
journal, July 1951
- Pohl, Herbert A.
- Journal of Applied Physics, Vol. 22, Issue 7
Selective detection of viable bacteria using dielectrophoretic impedance measurement method
journal, February 2003
- Suehiro, Junya; Hamada, Ryo; Noutomi, Daisuke
- Journal of Electrostatics, Vol. 57, Issue 2
A dielectrophoretic filter for separation and collection of fine particles suspended in liquid
conference, January 2002
- Zhou, G.; Imamura, M.; Suehiro, J.
- 2002 IEEE Industry Applications Society Annual Meeting, Conference Record of the 2002 IEEE Industry Applications Conference. 37th IAS Annual Meeting (Cat. No.02CH37344)
Improved Purification Methods for Calf-Derived Cryptosporidium parvum Oocysts Using Discontinuous Sucrose and Cesium Chloride Gradients
journal, September 1996
- Arrowood, Michael J.; Donaldson, Kimberley
- The Journal of Eukaryotic Microbiology, Vol. 43, Issue 5
Concentration of poliovirus in water by molecular filtration.
journal, January 1980
- Berman, D.; Rohr, M. E.; Safferman, R. S.
- Applied and Environmental Microbiology, Vol. 40, Issue 2
Manipulation and trapping of sub-micron bioparticles using dielectrophoresis
journal, September 1997
- Green, N. G.; Morgan, H.; Milner, Joel J.
- Journal of Biochemical and Biophysical Methods, Vol. 35, Issue 2
Optimization of a Reusable Hollow-Fiber Ultrafilter for Simultaneous Concentration of Enteric Bacteria, Protozoa, and Viruses from Water
journal, July 2003
- Morales-Morales, Hugo A.; Vidal, Guadalupe; Olszewski, John
- Applied and Environmental Microbiology, Vol. 69, Issue 7
High frequency electric fields for trapping of viruses
journal, April 1996
- M�ller, Torsten; Fiedler, Stefan; Schnelle, Thomas
- Biotechnology Techniques, Vol. 10, Issue 4
An insulator-based (electrodeless) dielectrophoretic concentrator for microbes in water
journal, September 2005
- Lapizco-Encinas, Blanca H.; Davalos, Rafael V.; Simmons, Blake A.
- Journal of Microbiological Methods, Vol. 62, Issue 3
Dielectrophoretic separation and manipulation of live and heat-treated cells of Listeria on microfabricated devices with interdigitated electrodes
journal, September 2002
- Li, H.
- Sensors and Actuators B: Chemical, Vol. 86, Issue 2-3
Sodium tripolyphosphate and polyphosphate as dispersing agents for kaolin suspensions: rheological characterization
journal, March 2002
- Papo, A.; Piani, L.; Ricceri, R.
- Colloids and Surfaces A: Physicochemical and Engineering Aspects, Vol. 201, Issue 1-3
The removal of phages T1 and PP7, and poliovirus from fluids with hollow-fiber ultrafilters with molecular weight cut-offs of 50 000, 13 000, and 6000
journal, April 1995
- Oshima, Kevin H.; Evans-Strickfaden, Tammy T.; Highsmith, Anita K.
- Canadian Journal of Microbiology, Vol. 41, Issue 4-5
Concentration of Giardia lamblia cysts, Legionella pneumophila , Clostridium perfringens , human enteric viruses, and coliphages from large volumes of drinking water, using a single filtration
journal, October 1989
- Payment, Pierre; Bérubé, Alain; Perreault, Daniel
- Canadian Journal of Microbiology, Vol. 35, Issue 10
Dielectrophoretic Concentration and Separation of Live and Dead Bacteria in an Array of Insulators
journal, March 2004
- Lapizco-Encinas, Blanca H.; Simmons, Blake A.; Cummings, Eric B.
- Analytical Chemistry, Vol. 76, Issue 6
Virus Concentration from Water Using High-Rate Tangential-Flow Hollow Fiber Ultrafiltration
journal, May 1983
- Dziewulski, David M.; Belfort, Georges
- Water Science and Technology, Vol. 15, Issue 5
Manipulation of herpes simplex virus type 1 by dielectrophoresis
journal, September 1998
- Hughes, Michael P.; Morgan, Hywel; Rixon, Frazer J.
- Biochimica et Biophysica Acta (BBA) - General Subjects, Vol. 1425, Issue 1
Dielectrophoresis in Microchips Containing Arrays of Insulating Posts: Theoretical and Experimental Results
journal, September 2003
- Cummings, Eric B.; Singh, Anup K.
- Analytical Chemistry, Vol. 75, Issue 18
Particle dispersion for further Cryptosporidium and Giardia detection by flow cytometry
journal, September 2003
- Lepesteur, M.; Blasdall, S.; Ashbolt, N. J.
- Letters in Applied Microbiology, Vol. 37, Issue 3
Some Effects of Nonuniform Fields on Dielectrics
journal, August 1958
- Pohl, Herbert Ackland
- Journal of Applied Physics, Vol. 29, Issue 8
Reversible and irreversible surface charge modification of bacteria for facilitating transport through porous media
journal, November 1985
- Sharma, M. M.; Chang, Y. I.; Yen, T. F.
- Colloids and Surfaces, Vol. 16, Issue 2
Applications of dielectrophoresis in biotechnology
journal, October 1997
- Pethig, R.; Markx, G.
- Trends in Biotechnology, Vol. 15, Issue 10
High sensitive detection of biological cells using dielectrophoretic impedance measurement method combined with electropermeabilization
journal, November 2003
- Suehiro, Junya; Shutou, Masanori; Hatano, Tetsuji
- Sensors and Actuators B: Chemical, Vol. 96, Issue 1-2
Trapping of viruses in high-frequency electric field cages
journal, April 1996
- Schnelle, T.; M�ller, T.; Fiedler, S.
- Naturwissenschaften, Vol. 83, Issue 4
Selective detection of specific bacteria using dielectrophoretic impedance measurement method combined with an antigen–antibody reaction
journal, June 2003
- Suehiro, Junya; Noutomi, Daisuke; Shutou, Masanori
- Journal of Electrostatics, Vol. 58, Issue 3-4
Separation of Living and Dead Cells by Dielectrophoresis
journal, April 1966
- Pohl, H. A.; Hawk, I.
- Science, Vol. 152, Issue 3722
The potential of dielectrophoresis for the real-time detection of microorganisms in foods
journal, February 1995
- Bernard Betts, W.
- Trends in Food Science & Technology, Vol. 6, Issue 2
A lab-on-a-chip for cell detection and manipulation
journal, June 2003
- Medoro, G.; Manaresi, N.; Leonardi, A.
- IEEE Sensors Journal, Vol. 3, Issue 3
Dielectrophoretic manipulation of rod-shaped viral particles
journal, December 1997
- Morgan, Hywel; Green, Nicolas G.
- Journal of Electrostatics, Vol. 42, Issue 3
Virus concentration using hollow fiber membranes—II☆
journal, January 1976
- Belfort, G.; Rotem, Y.; Katzenelson, E.
- Water Research, Vol. 10, Issue 4
Dielectrophoretic analysis of microbes in water
journal, December 1998
- Betts, W. B.; Brown, A. P.
- Journal of Applied Microbiology, Vol. 85, Issue S1
Dielectrophoresis of Cells
journal, September 1971
- Pohl, Herbert A.; Crane, Joe S.
- Biophysical Journal, Vol. 11, Issue 9