Hybridization-based biosensor containing hairpin probes and use thereof
Abstract
A sensor chip that includes: a fluorescence quenching surface; a nucleic acid probe that contains first and second ends with the first end bound to the fluorescence quenching surface, and is characterized by being able to self-anneal into a hairpin conformation; and a first fluorophore bound to the second end of the first nucleic acid molecule. When the first nucleic acid molecule is in the hairpin conformation, the fluorescence quenching surface substantially quenches fluorescent emissions by the first fluorophore; and when the first nucleic acid molecule is in a non-hairpin conformation, fluorescent emissions by the fluorophore are substantially free of quenching by the fluorescence quenching surface. Various nucleic acid probes, methods of making the sensor chip, biological sensor devices that contain the sensor chip, and their methods of use are also disclosed.
- Inventors:
-
- Penfield, NY
- Rochester, NY
- Issue Date:
- Research Org.:
- Univ. of Rochester, NY (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1016139
- Patent Number(s):
- 7811764
- Application Number:
- 11/838,616
- Assignee:
- University of Rochester (Rochester, NY)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
- DOE Contract Number:
- FG02-02ER63410
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
Citation Formats
Miller, Benjamin L, and Strohsahl, Christopher M. Hybridization-based biosensor containing hairpin probes and use thereof. United States: N. p., 2010.
Web.
Miller, Benjamin L, & Strohsahl, Christopher M. Hybridization-based biosensor containing hairpin probes and use thereof. United States.
Miller, Benjamin L, and Strohsahl, Christopher M. Tue .
"Hybridization-based biosensor containing hairpin probes and use thereof". United States. https://www.osti.gov/servlets/purl/1016139.
@article{osti_1016139,
title = {Hybridization-based biosensor containing hairpin probes and use thereof},
author = {Miller, Benjamin L and Strohsahl, Christopher M},
abstractNote = {A sensor chip that includes: a fluorescence quenching surface; a nucleic acid probe that contains first and second ends with the first end bound to the fluorescence quenching surface, and is characterized by being able to self-anneal into a hairpin conformation; and a first fluorophore bound to the second end of the first nucleic acid molecule. When the first nucleic acid molecule is in the hairpin conformation, the fluorescence quenching surface substantially quenches fluorescent emissions by the first fluorophore; and when the first nucleic acid molecule is in a non-hairpin conformation, fluorescent emissions by the fluorophore are substantially free of quenching by the fluorescence quenching surface. Various nucleic acid probes, methods of making the sensor chip, biological sensor devices that contain the sensor chip, and their methods of use are also disclosed.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Oct 12 00:00:00 EDT 2010},
month = {Tue Oct 12 00:00:00 EDT 2010}
}
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Works referenced in this record:
Dissemination among staphylococci of DNA sequences associated with methicillin resistance.
journal, March 1994
- Archer, G. L.; Niemeyer, D. M.; Thanassi, J. A.
- Antimicrobial Agents and Chemotherapy, Vol. 38, Issue 3
Hybridization-Based Unquenching of DNA Hairpins on Au Surfaces: Prototypical “Molecular Beacon” Biosensors
journal, April 2003
- Du, Hui; Disney, Matthew D.; Miller, Benjamin L.
- Journal of the American Chemical Society, Vol. 125, Issue 14
A Single-Molecule Sensitive DNA Hairpin System Based on Intramolecular Electron Transfer
journal, July 2003
- Piestert, Oliver; Barsch, Hannes; Buschmann, Volker
- Nano Letters, Vol. 3, Issue 7
Molecular detection of antibiotic resistance: when and where?
journal, June 2005
- Woodford, Neil; Sundsfjord, Arnfinn
- Journal of Antimicrobial Chemotherapy, Vol. 56, Issue 2
Detection of methicillin resistance in staphylococci by using a DNA probe.
journal, September 1990
- Archer, G. L.; Pennell, E.
- Antimicrobial Agents and Chemotherapy, Vol. 34, Issue 9
Thermodynamic basis of the enhanced specificity of structured DNA probes
journal, May 1999
- Bonnet, G.; Tyagi, S.; Libchaber, A.
- Proceedings of the National Academy of Sciences, Vol. 96, Issue 11
Single-mismatch detection using gold-quenched fluorescent oligonucleotides
journal, April 2001
- Dubertret, Benoit; Calame, Michel; Libchaber, Albert J.
- Nature Biotechnology, Vol. 19, Issue 4