skip to main content
DOE Patents title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Hybridization-based biosensor containing hairpin probes and use thereof

Abstract

A sensor chip that includes: a fluorescence quenching surface; a nucleic acid probe that contains first and second ends with the first end bound to the fluorescence quenching surface, and is characterized by being able to self-anneal into a hairpin conformation; and a first fluorophore bound to the second end of the first nucleic acid molecule. When the first nucleic acid molecule is in the hairpin conformation, the fluorescence quenching surface substantially quenches fluorescent emissions by the first fluorophore; and when the first nucleic acid molecule is in a non-hairpin conformation, fluorescent emissions by the fluorophore are substantially free of quenching by the fluorescence quenching surface. Various nucleic acid probes, methods of making the sensor chip, biological sensor devices that contain the sensor chip, and their methods of use are also disclosed.

Inventors:
 [1];  [2]
  1. Penfield, NY
  2. Rochester, NY
Issue Date:
Research Org.:
Univ. of Rochester, NY (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1016139
Patent Number(s):
7811764
Application Number:
11/838,616
Assignee:
University of Rochester (Rochester, NY)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
DOE Contract Number:  
FG02-02ER63410
Resource Type:
Patent
Country of Publication:
United States
Language:
English

Citation Formats

Miller, Benjamin L, and Strohsahl, Christopher M. Hybridization-based biosensor containing hairpin probes and use thereof. United States: N. p., 2010. Web.
Miller, Benjamin L, & Strohsahl, Christopher M. Hybridization-based biosensor containing hairpin probes and use thereof. United States.
Miller, Benjamin L, and Strohsahl, Christopher M. Tue . "Hybridization-based biosensor containing hairpin probes and use thereof". United States. https://www.osti.gov/servlets/purl/1016139.
@article{osti_1016139,
title = {Hybridization-based biosensor containing hairpin probes and use thereof},
author = {Miller, Benjamin L and Strohsahl, Christopher M},
abstractNote = {A sensor chip that includes: a fluorescence quenching surface; a nucleic acid probe that contains first and second ends with the first end bound to the fluorescence quenching surface, and is characterized by being able to self-anneal into a hairpin conformation; and a first fluorophore bound to the second end of the first nucleic acid molecule. When the first nucleic acid molecule is in the hairpin conformation, the fluorescence quenching surface substantially quenches fluorescent emissions by the first fluorophore; and when the first nucleic acid molecule is in a non-hairpin conformation, fluorescent emissions by the fluorophore are substantially free of quenching by the fluorescence quenching surface. Various nucleic acid probes, methods of making the sensor chip, biological sensor devices that contain the sensor chip, and their methods of use are also disclosed.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2010},
month = {10}
}

Patent:

Save / Share:

Works referenced in this record:

Dissemination among staphylococci of DNA sequences associated with methicillin resistance.
journal, March 1994


FemA, a host-mediated factor essential for methicillin resistance in Staphylococcus aureus: Molecular cloning and characterization
journal, October 1989


Hybridization-Based Unquenching of DNA Hairpins on Au Surfaces:  Prototypical “Molecular Beacon” Biosensors
journal, April 2003


A Single-Molecule Sensitive DNA Hairpin System Based on Intramolecular Electron Transfer
journal, July 2003


Molecular detection of antibiotic resistance: when and where?
journal, June 2005


Detection of methicillin resistance in staphylococci by using a DNA probe.
journal, September 1990


Thermodynamic basis of the enhanced specificity of structured DNA probes
journal, May 1999


Single-mismatch detection using gold-quenched fluorescent oligonucleotides
journal, April 2001