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Title: Hybridization-based biosensor containing hairpin probes and use thereof

Abstract

A sensor chip that includes: a fluorescence quenching surface; a nucleic acid probe that contains first and second ends with the first end bound to the fluorescence quenching surface, and is characterized by being able to self-anneal into a hairpin conformation; and a first fluorophore bound to the second end of the first nucleic acid molecule. When the first nucleic acid molecule is in the hairpin conformation, the fluorescence quenching surface substantially quenches fluorescent emissions by the first fluorophore; and when the first nucleic acid molecule is in a non-hairpin conformation, fluorescent emissions by the fluorophore are substantially free of quenching by the fluorescence quenching surface. Various nucleic acid probes, methods of making the sensor chip, biological sensor devices that contain the sensor chip, and their methods of use are also disclosed.

Inventors:
 [1];  [2]
  1. (Penfield, NY)
  2. (Rochester, NY)
Issue Date:
Research Org.:
University of Rochester (Rochester, NY)
Sponsoring Org.:
USDOE
OSTI Identifier:
1016139
Patent Number(s):
7,811,764
Application Number:
11/838,616
Assignee:
University of Rochester (Rochester, NY) CHO
DOE Contract Number:  
FG02-02ER63410
Resource Type:
Patent
Country of Publication:
United States
Language:
English

Citation Formats

Miller, Benjamin L., and Strohsahl, Christopher M. Hybridization-based biosensor containing hairpin probes and use thereof. United States: N. p., 2010. Web.
Miller, Benjamin L., & Strohsahl, Christopher M. Hybridization-based biosensor containing hairpin probes and use thereof. United States.
Miller, Benjamin L., and Strohsahl, Christopher M. Tue . "Hybridization-based biosensor containing hairpin probes and use thereof". United States. https://www.osti.gov/servlets/purl/1016139.
@article{osti_1016139,
title = {Hybridization-based biosensor containing hairpin probes and use thereof},
author = {Miller, Benjamin L. and Strohsahl, Christopher M.},
abstractNote = {A sensor chip that includes: a fluorescence quenching surface; a nucleic acid probe that contains first and second ends with the first end bound to the fluorescence quenching surface, and is characterized by being able to self-anneal into a hairpin conformation; and a first fluorophore bound to the second end of the first nucleic acid molecule. When the first nucleic acid molecule is in the hairpin conformation, the fluorescence quenching surface substantially quenches fluorescent emissions by the first fluorophore; and when the first nucleic acid molecule is in a non-hairpin conformation, fluorescent emissions by the fluorophore are substantially free of quenching by the fluorescence quenching surface. Various nucleic acid probes, methods of making the sensor chip, biological sensor devices that contain the sensor chip, and their methods of use are also disclosed.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2010},
month = {10}
}

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