Flow-controlled magnetic particle manipulation
Abstract
Inventive methods and apparatus are useful for collecting magnetic materials in one or more magnetic fields and resuspending the particles into a dispersion medium, and optionally repeating collection/resuspension one or more times in the same or a different medium, by controlling the direction and rate of fluid flow through a fluid flow path. The methods provide for contacting derivatized particles with test samples and reagents, removal of excess reagent, washing of magnetic material, and resuspension for analysis, among other uses. The methods are applicable to a wide variety of chemical and biological materials that are susceptible to magnetic labeling, including, for example, cells, viruses, oligonucleotides, proteins, hormones, receptor-ligand complexes, environmental contaminants and the like.
- Inventors:
-
- West Richland, WA
- Richland, WA
- Las Vegas, NV
- Issue Date:
- Research Org.:
- Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1015209
- Patent Number(s):
- 7892856
- Application Number:
- US Patent Application 11/646,216
- Assignee:
- Battelle Memorial Institute (Richland, WA)
- Patent Classifications (CPCs):
-
B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01J - CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY
B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01L - CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- DOE Contract Number:
- AC06-76RL01830
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 36 MATERIALS SCIENCE
Citation Formats
Grate, Jay W, Bruckner-Lea, Cynthia J, and Holman, David A. Flow-controlled magnetic particle manipulation. United States: N. p., 2011.
Web.
Grate, Jay W, Bruckner-Lea, Cynthia J, & Holman, David A. Flow-controlled magnetic particle manipulation. United States.
Grate, Jay W, Bruckner-Lea, Cynthia J, and Holman, David A. Tue .
"Flow-controlled magnetic particle manipulation". United States. https://www.osti.gov/servlets/purl/1015209.
@article{osti_1015209,
title = {Flow-controlled magnetic particle manipulation},
author = {Grate, Jay W and Bruckner-Lea, Cynthia J and Holman, David A},
abstractNote = {Inventive methods and apparatus are useful for collecting magnetic materials in one or more magnetic fields and resuspending the particles into a dispersion medium, and optionally repeating collection/resuspension one or more times in the same or a different medium, by controlling the direction and rate of fluid flow through a fluid flow path. The methods provide for contacting derivatized particles with test samples and reagents, removal of excess reagent, washing of magnetic material, and resuspension for analysis, among other uses. The methods are applicable to a wide variety of chemical and biological materials that are susceptible to magnetic labeling, including, for example, cells, viruses, oligonucleotides, proteins, hormones, receptor-ligand complexes, environmental contaminants and the like.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Feb 22 00:00:00 EST 2011},
month = {Tue Feb 22 00:00:00 EST 2011}
}