Conditional sterility in plants
Abstract
The present disclosure provides methods, recombinant DNA molecules, recombinant host cells containing the DNA molecules, and transgenic plant cells, plant tissue and plants which contain and express at least one antisense or interference RNA specific for a thiamine biosynthetic coding sequence or a thiamine binding protein or a thiamine-degrading protein, wherein the RNA or thiamine binding protein is expressed under the regulatory control of a transcription regulatory sequence which directs expression in male and/or female reproductive tissue. These transgenic plants are conditionally sterile; i.e., they are fertile only in the presence of exogenous thiamine. Such plants are especially appropriate for use in the seed industry or in the environment, for example, for use in revegetation of contaminated soils or phytoremediation, especially when those transgenic plants also contain and express one or more chimeric genes which confer resistance to contaminants.
- Inventors:
-
- Athens, GA
- Taejeon, KR
- Issue Date:
- Research Org.:
- Univ. of Georgia, Athens, GA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1015196
- Patent Number(s):
- 7667096
- Application Number:
- 10/559,301
- Assignee:
- University of Georgia Research Foundation, Inc. (Athens, GA)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
- DOE Contract Number:
- FG07-96ER20257
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 2004 Jun 03
- Country of Publication:
- United States
- Language:
- English
Citation Formats
Meagher, Richard B, McKinney, Elizabeth, and Kim, Tehryung. Conditional sterility in plants. United States: N. p., 2010.
Web.
Meagher, Richard B, McKinney, Elizabeth, & Kim, Tehryung. Conditional sterility in plants. United States.
Meagher, Richard B, McKinney, Elizabeth, and Kim, Tehryung. Tue .
"Conditional sterility in plants". United States. https://www.osti.gov/servlets/purl/1015196.
@article{osti_1015196,
title = {Conditional sterility in plants},
author = {Meagher, Richard B and McKinney, Elizabeth and Kim, Tehryung},
abstractNote = {The present disclosure provides methods, recombinant DNA molecules, recombinant host cells containing the DNA molecules, and transgenic plant cells, plant tissue and plants which contain and express at least one antisense or interference RNA specific for a thiamine biosynthetic coding sequence or a thiamine binding protein or a thiamine-degrading protein, wherein the RNA or thiamine binding protein is expressed under the regulatory control of a transcription regulatory sequence which directs expression in male and/or female reproductive tissue. These transgenic plants are conditionally sterile; i.e., they are fertile only in the presence of exogenous thiamine. Such plants are especially appropriate for use in the seed industry or in the environment, for example, for use in revegetation of contaminated soils or phytoremediation, especially when those transgenic plants also contain and express one or more chimeric genes which confer resistance to contaminants.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2010},
month = {2}
}
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Works referenced in this record:
A combination of derepression of the lac operator-repressor system with positive induction by glucocorticoid and metal ions provides a high-level-inducible gene expression system based on the human metallothionein-IIA promoter.
journal, December 1990
- Hu, M. C.; Davidson, N.
- Molecular and Cellular Biology, Vol. 10, Issue 12
Fungal pathogen protection in potato by expression of a plant defensin peptide
journal, December 2000
- Gao, Ai-Guo; Hakimi, Salim M.; Mittanck, Cindy A.
- Nature Biotechnology, Vol. 18, Issue 12
Mercuric ion reduction and resistance in transgenic Arabidopsis thaliana plants expressing a modified bacterial merA gene.
journal, April 1996
- Rugh, C. L.; Wilde, H. D.; Stack, N. M.
- Proceedings of the National Academy of Sciences, Vol. 93, Issue 8
Regulation of S-Like Ribonuclease Levels in Arabidopsis. Antisense Inhibition of RNS1 or RNS2 Elevates Anthocyanin Accumulation
journal, January 1999
- Bariola, Pauline A.; MacIntosh, Gustavo C.; Green, Pamela J.
- Plant Physiology, Vol. 119, Issue 1
Thiamin-Binding Protein from Sunflower Seeds.
journal, January 1998
- Watanabe, Katsumi; Chikushi, Kumi; Adachi, Takashi
- Journal of Nutritional Science and Vitaminology, Vol. 44, Issue 5
Strong, constitutive expression of the Arabidopsis ACT2/ACT8 actin subclass in vegetative tissues
journal, July 1996
- An, Yong-Qiang; McDowell, John M.; Huang, Shurong
- The Plant Journal, Vol. 10, Issue 1
The Arabidopsis ACT7 Actin Gene Is Expressed in Rapidly Developing Tissues and Responds to Several External Stimuli
journal, July 1996
- McDowell, J. M.; An, Yq.; Huang, S.
- Plant Physiology, Vol. 111, Issue 3
Cell cycle-dependent association of Arabidopsis actin-related proteins AtARP4 and AtARP7 with the nucleus: Two nuclear ARPs of Arabidopsis
journal, February 2003
- Kandasamy, Muthugapatti K.; McKinney, Elizabeth C.; Meagher, Richard B.
- The Plant Journal, Vol. 33, Issue 5
Analysis of the genome sequence of the flowering plant Arabidopsis thaliana
journal, December 2000
- Arabidopsis Genome Initiative,
- Nature, Vol. 408, Issue 6814, p. 796-815
Subcellular Targeting of Methylmercury Lyase Enhances Its Specific Activity for Organic Mercury Detoxification in Plants
journal, January 2003
- Bizily, Scott P.; Kim, Tehryung; Kandasamy, Muthugapatti K.
- Plant Physiology, Vol. 131, Issue 2
Engineering tolerance and hyperaccumulation of arsenic in plants by combining arsenate reductase and γ-glutamylcysteine synthetase expression
journal, October 2002
- Dhankher, Om Parkash; Li, Yujing; Rosen, Barry P.
- Nature Biotechnology, Vol. 20, Issue 11
Operator DNA Sequence Variation Enhances High Affinity Binding by Hinge Helix Mutants of Lactose Repressor Protein †
journal, September 2000
- Falcon, Catherine M.; Matthews, Kathleen S.
- Biochemistry, Vol. 39, Issue 36
Functional Nonequivalency of Actin Isovariants in Arabidopsis
journal, January 2002
- Kandasamy, Muthugapatti K.; McKinney, Elizabeth C.; Meagher, Richard B.
- Molecular Biology of the Cell, Vol. 13, Issue 1
Aspartate-27 and glutamate-473 are involved in catalysis by Zymomonas mobilis pyruvate decarboxylase
journal, April 1999
- Chang, Alan K.; Nixon, Peter F.; Duggleby, Ronald G.
- Biochemical Journal, Vol. 339, Issue 2
A Brassica cDNA clone encoding a bifunctional hydroxymethylpyrimidine kinase/thiamin-phosphate pyrophosphorylase involved in thiamin biosynthesis
journal, August 1998
- Suk Kim, Yang; Nosaka, Kazuto; Downs, Diana M.
- Plant Molecular Biology, Vol. 37, Issue 6, p. 955-966
DNA-mediated genetic correction of thiamineless Arabidopsis thaliana
journal, May 1974
- Ledoux, L.; Huart, R.; Jacobs, M.
- Nature, Vol. 249, Issue 5452
Codon usage in plant genes
journal, January 1989
- Murray, Elizabeth E.; Lotzer, Jeff; Eberle, Mary
- Nucleic Acids Research, Vol. 17, Issue 2
SCARECROW is involved in positioning the stem cell niche in the Arabidopsis root meristem
journal, February 2003
- Sabatini, S.
- Genes & Development, Vol. 17, Issue 3
Tapetum-Specific Expression of the Gene for an Endo-β-1,3-glucanase Causes Male Sterility in Transgenic Tobacco
journal, April 1995
- Tsuchiya, Tohru; Toriyama, Kinya; Yoshikawa, Masaaki
- Plant and Cell Physiology, Vol. 36, Issue 3
Hybrid seed production and the challenge of propagating male-sterile plants
journal, May 2002
- Perez-Prat, Eva; van Lookeren Campagne, Michiel M.
- Trends in Plant Science, Vol. 7, Issue 5
The Pyruvate decarboxylase1 Gene of Arabidopsis Is Required during Anoxia But Not Other Environmental Stresses
journal, April 2003
- Kürsteiner, Oliver; Dupuis, Isabelle; Kuhlemeier, Cris
- Plant Physiology, Vol. 132, Issue 2
Phytoremediation of Mercury- and Methylmercury-Polluted Soils Using Genetically Engineered Plants
journal, July 1998
- Heaton, Andrew C. P.; Rugh, Clayton L.; Wang, Nian-jie
- Journal of Soil Contamination, Vol. 7, Issue 4
Thiamine mutants of the crucifer, Arabidopsis
journal, April 1969
- Li, S. L.; R�dei, G. P.
- Biochemical Genetics, Vol. 3, Issue 2
The Arabidopsis ACT11 actin gene is strongly expressed in tissues of the emerging inflorescence, pollen, and developing ovules
journal, January 1997
- Huang, Shurong; An, Yong-Qiang; McDowell, John M.
- Plant Molecular Biology, Vol. 33, Issue 1, p. 125-139
Root-specific genes from tobacco and Arabidopsis homologous to an evolutionarily conserved gene family of membrane channel proteins
journal, January 1990
- Yamamoto, Yuri T.; Cheng, Chi-Lien; Concking, Mark A.
- Nucleic Acids Research, Vol. 18, Issue 24
Transfer of the inducible lac repressor/operator system from Escherichia coli to a vaccinia virus expression vector.
journal, April 1989
- Fuerst, T. R.; Fernandez, M. P.; Moss, B.
- Proceedings of the National Academy of Sciences, Vol. 86, Issue 8
A positive regulatory gene, THI3, is required for thiamine metabolism in Saccharomyces cerevisiae.
journal, January 1992
- Nishimura, H.; Kawasaki, Y.; Kaneko, Y.
- Journal of Bacteriology, Vol. 174, Issue 14
Ligand–Protein Interaction in Plant Seed Thiamine-Binding Proteins. Binding of Various Thiamine Analogues to the Sepharose-Immobilized Buckwheat-Seed Protein
journal, August 1999
- Rapala-Kozik, Maria; Chernikevich, Ivan P.; Kozik, Andrzej
- Journal of Protein Chemistry, Vol. 18, Issue 6, p. 721-728
Development of transgenic yellow poplar for mercury phytoremediation
journal, October 1998
- Rugh, Clayton L.; Senecoff, Julie F.; Meagher, Richard B.
- Nature Biotechnology, Vol. 16, Issue 10
A constitutive thiamine metabolism mutation, thi80, causing reduced thiamine pyrophosphokinase activity in Saccharomyces cerevisiae.
journal, January 1991
- Nishimura, H.; Kawasaki, Y.; Nosaka, K.
- Journal of Bacteriology, Vol. 173, Issue 8
Phytodetoxification of hazardous organomercurials by genetically engineered plants
journal, February 2000
- Bizily, Scott P.; Rugh, Clayton L.; Meagher, Richard B.
- Nature Biotechnology, Vol. 18, Issue 2
5′ proximal sequences of a soybean ribulose-1, 5-bisphosphate carboxylase small subunit gene direct light and phytochrome controlled transcription
journal, January 1987
- Shirley, B. W.; Berry-Lowe, S. L.; Rogers, S. G.
- Nucleic Acids Research, Vol. 15, Issue 16
Regulated expression of plant tRNA genes by the prokaryotic tet and lac repressors
journal, November 1997
- Ulmasov, Barbara; Capone, John; Folk*, William
- Plant Molecular Biology, Vol. 35, Issue 4, p. 417-424
The Arabidopsis thaliana ACT4/ACT12 actin gene subclass is strongly expressed throughout pollen development
journal, August 1996
- Huang, Shurong; An, Yong-Qiang; McDowell, John M.
- The Plant Journal, Vol. 10, Issue 2
A Species of Small Antisense RNA in Posttranscriptional Gene Silencing in Plants
journal, October 1999
- Hamilton, A. J.
- Science, Vol. 286, Issue 5441
Dedicated Roles of Plastid Transketolases during the Early Onset of Isoprenoid Biogenesis in Pepper Fruits 1
journal, August 1998
- Bouvier, Florence; d'Harlingue, Alain; Suire, Claude
- Plant Physiology, Vol. 117, Issue 4
Specific and heritable genetic interference by double-stranded RNA in Arabidopsis thaliana
journal, April 2000
- Chuang, C.-F.; Meyerowitz, E. M.
- Proceedings of the National Academy of Sciences, Vol. 97, Issue 9, p. 4985-4990
Toxic Mercury Reduction and Remediation Using Transgenic Plants with a Modified Bacterial Gene
journal, July 1998
- Rugh, Clayton L.; Gragson, Gay M.; Meagher, Richard B.
- HortScience, Vol. 33, Issue 4
Characterization of Thiamin-Binding Protein from Buckwheat Seeds.
journal, January 1998
- Watanabe, Katsumi; Shimizu, Mayumi; Adachi, Takashi
- Journal of Nutritional Science and Vitaminology, Vol. 44, Issue 2
nmt2 of fission yeast: A second thiamine-repressible gene co-ordinately regulated withnmt1
journal, August 1994
- Manetti, Andrea G. O.; Rosetto, Marco; Maundrell, Kinsey G.
- Yeast, Vol. 10, Issue 8
Base substitution mutants of the lac operator: in vivo and in vitro affinities for lac repressor
journal, January 1986
- Betz, Joan L.; Sasmor, Henri M.; Buck, Fritz
- Gene, Vol. 50, Issue 1-3
Suppression of gene silencing: A general strategy used by diverse DNA and RNA viruses of plants
journal, November 1999
- Voinnet, O.; Pinto, Y. M.; Baulcombe, D. C.
- Proceedings of the National Academy of Sciences, Vol. 96, Issue 24
Isolation of Transcriptionally Regulated Root-Specific Genes from Tobacco
journal, July 1990
- Conkling, M. A.; Cheng, C.-l.; Yamamoto, Y. T.
- Plant Physiology, Vol. 93, Issue 3, p. 1203-1211
Characterization of cis-acting sequences regulating root-specific gene expression in tobacco.
journal, April 1991
- Yamamoto, Y. T.; Taylor, C. G.; Acedo, G. N.
- The Plant Cell, Vol. 3, Issue 4
GUS fusions: beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants.
journal, December 1987
- Jefferson, R. A.; Kavanagh, T. A.; Bevan, M. W.
- The EMBO Journal, Vol. 6, Issue 13
A chimaeric ribonuclease-inhibitor gene restores fertility to male sterile plants
journal, June 1992
- Mariani, Celestina; Gossele, Veronique; Beuckeleer, Marc De
- Nature, Vol. 357, Issue 6377
Hybrid seed production using nutritional mutants
journal, February 1991
- Barab�s, Zolt�n
- Euphytica, Vol. 53, Issue 1
Phytoremediation of methylmercury pollution: merB expression in Arabidopsis thaliana confers resistance to organomercurials
journal, June 1999
- Bizily, S. P.; Rugh, C. L.; Summers, A. O.
- Proceedings of the National Academy of Sciences, Vol. 96, Issue 12
Root-specific expression of a Zea mays gene encoding a novel glycine-rich protein, zmGRP3
journal, March 1998
- Goddemeier, Markus L.; Wulff, Dörte; Feix, Günter
- Plant Molecular Biology, Vol. 36, Issue 5, p. 799-802
Life with 6000 Genes
journal, October 1996
- Goffeau, A.; Barrell, B. G.; Bussey, H.
- Science, Vol. 274, Issue 5287
Root-specific expression of theLeRse-1 gene in tomato is induced by exposure of the shoot to light
journal, October 1996
- Lauter, F. R.
- Molecular and General Genetics MGG, Vol. 252, Issue 6
Systemic silencing signal(s)
journal, June 2000
- Fagard, Mathilde; Vaucheret, Hervé
- Plant Molecular Biology, Vol. 43, Issue 2/3, p. 285-293
Thi1, a thiamine biosynthetic gene inArabidopsis thaliana, complements bacterial defects in DNA repair
journal, June 1996
- Machado, C. R.; Costa de Oliveira, R. L.; Boiteux, S.
- Plant Molecular Biology, Vol. 31, Issue 3
A Viral Movement Protein Prevents Spread of the Gene Silencing Signal in Nicotiana benthamiana
journal, September 2000
- Voinnet, Olivier; Lederer, Carsten; Baulcombe, David C.
- Cell, Vol. 103, Issue 1
An mRNA Putatively Coding for an O-Methyltransferase Accumulates Preferentially in Maize Roots and Is Located Predominantly in the Region of the Endodermis
journal, July 1993
- Held, B. M.; Wang, H.; John, I.
- Plant Physiology, Vol. 102, Issue 3
Hydroxyethylthiazole kinase
book, January 1997
- Schomburg, Dietmar; Stephan, Dörte
- Enzyme Handbook 13
Bcp1, a gene required for male fertility in Arabidopsis.
journal, March 1995
- Xu, H.; Knox, R. B.; Taylor, P. E.
- Proceedings of the National Academy of Sciences, Vol. 92, Issue 6
Expression, purification and characterization of Arabidopsis thaliana acetohydroxyacid synthase
journal, October 1997
- Chang, Alan K.; Duggleby, Ronald G.
- Biochemical Journal, Vol. 327, Issue 1
Dual role for the yeast THI4 gene in thiamine biosynthesis and DNA damage tolerance
journal, October 1997
- Machado, Carlos R.; Praekelt, Uta M.; de Oliveira, Regina Costa
- Journal of Molecular Biology, Vol. 273, Issue 1
Arabidopsis Contains Ancient Classes of Differentially Expressed Actin-Related Protein Genes
journal, March 2002
- McKinney, Elizabeth Cohen; Kandasamy, Muthugapatti K.; Meagher, Richard B.
- Plant Physiology, Vol. 128, Issue 3
Phytoremediation of toxic elemental and organic pollutants
journal, April 2000
- Meagher, Richard B.
- Current Opinion in Plant Biology, Vol. 3, Issue 2
Enhancement of foreign gene expression by a dicot intron in rice but not in tobacco is correlated with an increased level of mRNA and an efficient splicing of the intron
journal, January 1990
- Tanaka, Akira; Mita, Satoru; Ohta, Shozo
- Nucleic Acids Research, Vol. 18, Issue 23
The late pollen-specific actins in angiosperms
journal, June 1999
- Kandasamy, Muthugapatti K.; McKinney, Elizabeth C.; Meagher, Richard B.
- The Plant Journal, Vol. 18, Issue 6
Tetrad analysis possible in Arabidopsis with mutation of the QUARTET (QRT) genes
journal, June 1994
- Preuss, D.; Rhee, S.; Davis, R.
- Science, Vol. 264, Issue 5164
Synthetic peptides as nuclear localization signals
journal, August 1986
- Goldfarb, David S.; Gariépy, Jean; Schoolnik, Gary
- Nature, Vol. 322, Issue 6080
A pyramid of loci for partial resistance to Fusarium solani f. sp. glycines maintains Myo-inositol-1-phosphate synthase expression in soybean roots
journal, December 2002
- Iqbal, M.; Afzal, A.; Yaegashi, S.
- Theoretical and Applied Genetics, Vol. 105, Issue 8
Small Changes in the Regulation of One Arabidopsis Profilin Isovariant, PRF1, Alter Seedling Development
journal, May 2001
- McKinney, Elizabeth Cohen; Kandasamy, Muthugapatti K.; Meagher, Richard B.
- The Plant Cell, Vol. 13, Issue 5
Positive Selection for Male-Sterile Mutants of Arabidopsis Lacking Adenine Phosphoribosyl Transferase Activity
journal, April 1988
- Moffatt, Barbara; Somerville, Chris
- Plant Physiology, Vol. 86, Issue 4
The Arabidopsis Cytoskeletal Genome
journal, January 2003
- Meagher, Richard B.; Fechheimer, Marcus
- The Arabidopsis Book, Vol. 2
Pyk10, a seedling and root specific gene and promoter from Arabidopsis thaliana
journal, July 2001
- Nitz, Inke; Berkefeld, Heike; Puzio, Piotr S.
- Plant Science, Vol. 161, Issue 2
Dual targeting properties of the N-terminal signal sequence of Arabidopsis thaliana THI1 protein to mitochondria and chloroplasts
journal, August 2001
- Chabregas, Sabrina M.; Luche, Douglas D.; Farias, Leonardo P.
- Plant Molecular Biology, Vol. 46, Issue 6, p. 639-650
zrp2: a novel maize gene whose mRNA accumulates in the root cortex and mature stems
journal, October 1997
- Held, Bruce M.; John, Isaac; Wang, Huiquing
- Plant Molecular Biology, Vol. 35, Issue 3, p. 367-375