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Title: Mass spectrometry-based methods for detection and differentiation of botulinum neurotoxins

Abstract

The present invention is directed to a method for detecting the presence of clostridial neurotoxins in a sample by mixing a sample with a peptide that can serve as a substrate for proteolytic activity of a clostridial neurotoxin; and measuring for proteolytic activity of a clostridial neurotoxin by a mass spectroscopy technique. In one embodiment, the peptide can have an affinity tag attached at two or more sites.

Inventors:
 [1];  [2];  [2];  [3];  [4];  [2]
  1. Los Alamos, NM
  2. Atlanta, GA
  3. Tucker, GA
  4. Suwannee, GA
Issue Date:
Research Org.:
Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1015176
Patent Number(s):
7611856
Application Number:
US Patent Application 10/980,346
Assignee:
Los Alamos National Security, LLC (Los Alamos, NM)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
DOE Contract Number:  
W-7405-ENG-36
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES

Citation Formats

Schmidt, Jurgen G, Boyer, Anne E, Kalb, Suzanne R, Moura, Hercules, Barr, John R, and Woolfitt, Adrian R. Mass spectrometry-based methods for detection and differentiation of botulinum neurotoxins. United States: N. p., 2009. Web.
Schmidt, Jurgen G, Boyer, Anne E, Kalb, Suzanne R, Moura, Hercules, Barr, John R, & Woolfitt, Adrian R. Mass spectrometry-based methods for detection and differentiation of botulinum neurotoxins. United States.
Schmidt, Jurgen G, Boyer, Anne E, Kalb, Suzanne R, Moura, Hercules, Barr, John R, and Woolfitt, Adrian R. Tue . "Mass spectrometry-based methods for detection and differentiation of botulinum neurotoxins". United States. https://www.osti.gov/servlets/purl/1015176.
@article{osti_1015176,
title = {Mass spectrometry-based methods for detection and differentiation of botulinum neurotoxins},
author = {Schmidt, Jurgen G and Boyer, Anne E and Kalb, Suzanne R and Moura, Hercules and Barr, John R and Woolfitt, Adrian R},
abstractNote = {The present invention is directed to a method for detecting the presence of clostridial neurotoxins in a sample by mixing a sample with a peptide that can serve as a substrate for proteolytic activity of a clostridial neurotoxin; and measuring for proteolytic activity of a clostridial neurotoxin by a mass spectroscopy technique. In one embodiment, the peptide can have an affinity tag attached at two or more sites.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2009},
month = {11}
}

Works referenced in this record:

Macromolecular Chromogenic Substrates for Measuring Proteinase Activity
journal, February 2001


Fluorigenic Substrates for the Protease Activities of Botulinum Neurotoxins, Serotypes A, B, and F
journal, January 2003


High-Throughput Assays for Botulinum Neurotoxin Proteolytic Activity: Serotypes A, B, D, and F
journal, September 2001


Isotope Dilution Mass Spectrometry and the National Reference System
journal, June 1993