Mass spectrometry-based methods for detection and differentiation of botulinum neurotoxins
Abstract
The present invention is directed to a method for detecting the presence of clostridial neurotoxins in a sample by mixing a sample with a peptide that can serve as a substrate for proteolytic activity of a clostridial neurotoxin; and measuring for proteolytic activity of a clostridial neurotoxin by a mass spectroscopy technique. In one embodiment, the peptide can have an affinity tag attached at two or more sites.
- Inventors:
-
- Los Alamos, NM
- Atlanta, GA
- Tucker, GA
- Suwannee, GA
- Issue Date:
- Research Org.:
- Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1015176
- Patent Number(s):
- 7611856
- Application Number:
- US Patent Application 10/980,346
- Assignee:
- Los Alamos National Security, LLC (Los Alamos, NM)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- DOE Contract Number:
- W-7405-ENG-36
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 60 APPLIED LIFE SCIENCES
Citation Formats
Schmidt, Jurgen G, Boyer, Anne E, Kalb, Suzanne R, Moura, Hercules, Barr, John R, and Woolfitt, Adrian R. Mass spectrometry-based methods for detection and differentiation of botulinum neurotoxins. United States: N. p., 2009.
Web.
Schmidt, Jurgen G, Boyer, Anne E, Kalb, Suzanne R, Moura, Hercules, Barr, John R, & Woolfitt, Adrian R. Mass spectrometry-based methods for detection and differentiation of botulinum neurotoxins. United States.
Schmidt, Jurgen G, Boyer, Anne E, Kalb, Suzanne R, Moura, Hercules, Barr, John R, and Woolfitt, Adrian R. Tue .
"Mass spectrometry-based methods for detection and differentiation of botulinum neurotoxins". United States. https://www.osti.gov/servlets/purl/1015176.
@article{osti_1015176,
title = {Mass spectrometry-based methods for detection and differentiation of botulinum neurotoxins},
author = {Schmidt, Jurgen G and Boyer, Anne E and Kalb, Suzanne R and Moura, Hercules and Barr, John R and Woolfitt, Adrian R},
abstractNote = {The present invention is directed to a method for detecting the presence of clostridial neurotoxins in a sample by mixing a sample with a peptide that can serve as a substrate for proteolytic activity of a clostridial neurotoxin; and measuring for proteolytic activity of a clostridial neurotoxin by a mass spectroscopy technique. In one embodiment, the peptide can have an affinity tag attached at two or more sites.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2009},
month = {11}
}
Save to My Library
You must Sign In or Create an Account in order to save documents to your library.
Works referenced in this record:
From the Mouse to the Mass Spectrometer: Detection and Differentiation of the Endoproteinase Activities of Botulinum Neurotoxins A−G by Mass Spectrometry
journal, July 2005
- Boyer, Anne E.; Moura, Hercules; Woolfitt, Adrian R.
- Analytical Chemistry, Vol. 77, Issue 13
Macromolecular Chromogenic Substrates for Measuring Proteinase Activity
journal, February 2001
- Hortin, Glen L.; Warshawsky, Ilka; Laude-Sharp, Maryline
- Clinical Chemistry, Vol. 47, Issue 2
Fluorigenic Substrates for the Protease Activities of Botulinum Neurotoxins, Serotypes A, B, and F
journal, January 2003
- Schmidt, James J.; Stafford, Robert G.
- Applied and Environmental Microbiology, Vol. 69, Issue 1
High-Throughput Assays for Botulinum Neurotoxin Proteolytic Activity: Serotypes A, B, D, and F
journal, September 2001
- Schmidt, James J.; Stafford, Robert G.; Millard, Charles B.
- Analytical Biochemistry, Vol. 296, Issue 1
Isotope Dilution Mass Spectrometry and the National Reference System
journal, June 1993
- Bowers, George N.; Fassett, John D.; White, Edward.
- Analytical Chemistry, Vol. 65, Issue 12