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Title: Analyte detection using an active assay

Abstract

Analytes using an active assay may be detected by introducing an analyte solution containing a plurality of analytes to a lacquered membrane. The lacquered membrane may be a membrane having at least one surface treated with a layer of polymers. The lacquered membrane may be semi-permeable to nonanalytes. The layer of polymers may include cross-linked polymers. A plurality of probe molecules may be arrayed and immobilized on the lacquered membrane. An external force may be applied to the analyte solution to move the analytes towards the lacquered membrane. Movement may cause some or all of the analytes to bind to the lacquered membrane. In cases where probe molecules are presented, some or all of the analytes may bind to probe molecules. The direction of the external force may be reversed to remove unbound or weakly bound analytes. Bound analytes may be detected using known detection types.

Inventors:
 [1];  [2];  [3]
  1. Manassas, VA
  2. Cross Junction, VA
  3. Potomac Falls, VA
Issue Date:
Research Org.:
George Mason Intellectual Properties, Inc. (Fairfax, VA)
Sponsoring Org.:
USDOE
OSTI Identifier:
1014958
Patent Number(s):
7824927
Application Number:
US Patent Application 11/397,905
Assignee:
George Mason Intellectual Properties, Inc. (Fairfax, VA)
Patent Classifications (CPCs):
Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y10 - TECHNICAL SUBJECTS COVERED BY FORMER USPC Y10T - TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
DOE Contract Number:  
FC52-04NA25455
Resource Type:
Patent
Country of Publication:
United States
Language:
English

Citation Formats

Morozov, Victor, Bailey, Charles L, and Evanskey, Melissa R. Analyte detection using an active assay. United States: N. p., 2010. Web.
Morozov, Victor, Bailey, Charles L, & Evanskey, Melissa R. Analyte detection using an active assay. United States.
Morozov, Victor, Bailey, Charles L, and Evanskey, Melissa R. Tue . "Analyte detection using an active assay". United States. https://www.osti.gov/servlets/purl/1014958.
@article{osti_1014958,
title = {Analyte detection using an active assay},
author = {Morozov, Victor and Bailey, Charles L and Evanskey, Melissa R},
abstractNote = {Analytes using an active assay may be detected by introducing an analyte solution containing a plurality of analytes to a lacquered membrane. The lacquered membrane may be a membrane having at least one surface treated with a layer of polymers. The lacquered membrane may be semi-permeable to nonanalytes. The layer of polymers may include cross-linked polymers. A plurality of probe molecules may be arrayed and immobilized on the lacquered membrane. An external force may be applied to the analyte solution to move the analytes towards the lacquered membrane. Movement may cause some or all of the analytes to bind to the lacquered membrane. In cases where probe molecules are presented, some or all of the analytes may bind to probe molecules. The direction of the external force may be reversed to remove unbound or weakly bound analytes. Bound analytes may be detected using known detection types.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2010},
month = {11}
}

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