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Title: Multiplex detection of agricultural pathogens

Abstract

Described are kits and methods useful for detection of seven agricultural pathogens (BPSV; BHV; BVD; FMDV; BTV; SVD; and VESV) in a sample. Genomic sequence information from 7 agricultural pathogens was analyzed to identify signature sequences, e.g., polynucleotide sequences useful for confirming the presence or absence of a pathogen in a sample. Primer and probe sets were designed and optimized for use in a PCR based, multiplexed Luminex assay to successfully identify the presence or absence of pathogens in a sample.

Inventors:
 [1];  [2];  [3]
  1. Brentwood, CA
  2. Livermore, CA
  3. Kensington, CA
Issue Date:
Research Org.:
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1014706
Patent Number(s):
7794938
Application Number:
US Patent Application 11/652,841
Assignee:
Lawrence Livermore National Security, LLC (Livermore, CA)
Patent Classifications (CPCs):
Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y10 - TECHNICAL SUBJECTS COVERED BY FORMER USPC Y10S - TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
DOE Contract Number:  
W-7405-ENG-48
Resource Type:
Patent
Country of Publication:
United States
Language:
English

Citation Formats

McBride, Mary Teresa, Slezak, Thomas Richard, and Messenger, Sharon Lee. Multiplex detection of agricultural pathogens. United States: N. p., 2010. Web.
McBride, Mary Teresa, Slezak, Thomas Richard, & Messenger, Sharon Lee. Multiplex detection of agricultural pathogens. United States.
McBride, Mary Teresa, Slezak, Thomas Richard, and Messenger, Sharon Lee. Tue . "Multiplex detection of agricultural pathogens". United States. https://www.osti.gov/servlets/purl/1014706.
@article{osti_1014706,
title = {Multiplex detection of agricultural pathogens},
author = {McBride, Mary Teresa and Slezak, Thomas Richard and Messenger, Sharon Lee},
abstractNote = {Described are kits and methods useful for detection of seven agricultural pathogens (BPSV; BHV; BVD; FMDV; BTV; SVD; and VESV) in a sample. Genomic sequence information from 7 agricultural pathogens was analyzed to identify signature sequences, e.g., polynucleotide sequences useful for confirming the presence or absence of a pathogen in a sample. Primer and probe sets were designed and optimized for use in a PCR based, multiplexed Luminex assay to successfully identify the presence or absence of pathogens in a sample.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2010},
month = {9}
}

Works referenced in this record:

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Research Profiles: Liquid array single-handedly detects bounty of BW agents
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A multiplexed PCR-coupled liquid bead array for the simultaneous detection of four biothreat agents
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Multiplexed Liquid Arrays for Simultaneous Detection of Simulants of Biological Warfare Agents
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A multiplex DNA suspension microarray for simultaneous detection and differentiation of classical swine fever virus and other pestiviruses
journal, September 2006


Detection of all seven serotypes of foot-and-mouth disease virus by real-time, fluorogenic reverse transcription polymerase chain reaction assay
journal, August 2002