Methods for validating the presence of and characterizing proteins deposited onto an array
Abstract
A method of determining if proteins have been transferred from liquid-phase protein fractions to an array comprising staining the array with a total protein stain and imaging the array, optionally comparing the staining with a standard curve generated by staining known amounts of a known protein on the same or a similar array; a method of characterizing proteins transferred from liquid-phase protein fractions to an array including staining the array with a post-translational modification-specific (PTM-specific) stain and imaging the array and, optionally, after staining the array with a PTM-specific stain and imaging the array, washing the array, re-staining the array with a total protein stain, imaging the array, and comparing the imaging with the PTM-specific stain with the imaging with the total protein stain; stained arrays; and images of stained arrays.
- Inventors:
-
- Naperville, IL
- Issue Date:
- Research Org.:
- Argonne National Laboratory (ANL), Argonne, IL (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1014655
- Patent Number(s):
- 7799570
- Application Number:
- 11/693,320
- Assignee:
- Uchicago Argonne, LLC (Chicago, IL)
- Patent Classifications (CPCs):
-
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- DOE Contract Number:
- W-31-109-ENG-38
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 2007 Mar 29
- Country of Publication:
- United States
- Language:
- English
Citation Formats
Schabacker, Daniel S. Methods for validating the presence of and characterizing proteins deposited onto an array. United States: N. p., 2010.
Web.
Schabacker, Daniel S. Methods for validating the presence of and characterizing proteins deposited onto an array. United States.
Schabacker, Daniel S. Tue .
"Methods for validating the presence of and characterizing proteins deposited onto an array". United States. https://www.osti.gov/servlets/purl/1014655.
@article{osti_1014655,
title = {Methods for validating the presence of and characterizing proteins deposited onto an array},
author = {Schabacker, Daniel S.},
abstractNote = {A method of determining if proteins have been transferred from liquid-phase protein fractions to an array comprising staining the array with a total protein stain and imaging the array, optionally comparing the staining with a standard curve generated by staining known amounts of a known protein on the same or a similar array; a method of characterizing proteins transferred from liquid-phase protein fractions to an array including staining the array with a post-translational modification-specific (PTM-specific) stain and imaging the array and, optionally, after staining the array with a PTM-specific stain and imaging the array, washing the array, re-staining the array with a total protein stain, imaging the array, and comparing the imaging with the PTM-specific stain with the imaging with the total protein stain; stained arrays; and images of stained arrays.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2010},
month = {9}
}
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