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Title: Methods for validating the presence of and characterizing proteins deposited onto an array

Abstract

A method of determining if proteins have been transferred from liquid-phase protein fractions to an array comprising staining the array with a total protein stain and imaging the array, optionally comparing the staining with a standard curve generated by staining known amounts of a known protein on the same or a similar array; a method of characterizing proteins transferred from liquid-phase protein fractions to an array including staining the array with a post-translational modification-specific (PTM-specific) stain and imaging the array and, optionally, after staining the array with a PTM-specific stain and imaging the array, washing the array, re-staining the array with a total protein stain, imaging the array, and comparing the imaging with the PTM-specific stain with the imaging with the total protein stain; stained arrays; and images of stained arrays.

Inventors:
 [1]
  1. Naperville, IL
Issue Date:
Research Org.:
Argonne National Laboratory (ANL), Argonne, IL (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1014655
Patent Number(s):
7799570
Application Number:
11/693,320
Assignee:
Uchicago Argonne, LLC (Chicago, IL)
Patent Classifications (CPCs):
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
DOE Contract Number:  
W-31-109-ENG-38
Resource Type:
Patent
Resource Relation:
Patent File Date: 2007 Mar 29
Country of Publication:
United States
Language:
English

Citation Formats

Schabacker, Daniel S. Methods for validating the presence of and characterizing proteins deposited onto an array. United States: N. p., 2010. Web.
Schabacker, Daniel S. Methods for validating the presence of and characterizing proteins deposited onto an array. United States.
Schabacker, Daniel S. Tue . "Methods for validating the presence of and characterizing proteins deposited onto an array". United States. https://www.osti.gov/servlets/purl/1014655.
@article{osti_1014655,
title = {Methods for validating the presence of and characterizing proteins deposited onto an array},
author = {Schabacker, Daniel S.},
abstractNote = {A method of determining if proteins have been transferred from liquid-phase protein fractions to an array comprising staining the array with a total protein stain and imaging the array, optionally comparing the staining with a standard curve generated by staining known amounts of a known protein on the same or a similar array; a method of characterizing proteins transferred from liquid-phase protein fractions to an array including staining the array with a post-translational modification-specific (PTM-specific) stain and imaging the array and, optionally, after staining the array with a PTM-specific stain and imaging the array, washing the array, re-staining the array with a total protein stain, imaging the array, and comparing the imaging with the PTM-specific stain with the imaging with the total protein stain; stained arrays; and images of stained arrays.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2010},
month = {9}
}

Works referenced in this record:

Strategies and Solid-Phase Formats for the Analysis of Protein and Peptide Phosphorylation Employing A Novel Fluorescent Phosphorylation Sensor Dye
journal, June 2003


Protein microarrays using liquid phase fractionation of cell lysates
journal, July 2003


Protein array staining methods for undefined protein content, manufacturing quality control, and performance validation
journal, December 2006


Signal pathway profiling of prostate cancer using reverse phase protein arrays
journal, November 2003


Use of protein–acrylamide copolymer hydrogels for measuring protein concentration and activity
journal, June 2004