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Title: Cysteine-containing peptide tag for site-specific conjugation of proteins

Abstract

The present invention is directed to a biological conjugate, comprising: (a) a targeting moiety comprising a polypeptide having an amino acid sequence comprising the polypeptide sequence of SEQ ID NO:2 and the polypeptide sequence of a selected targeting protein; and (b) a binding moiety bound to the targeting moiety; the biological conjugate having a covalent bond between the thiol group of SEQ ID NO:2 and a functional group in the binding moiety. The present invention is directed to a biological conjugate, comprising: (a) a targeting moiety comprising a polypeptide having an amino acid sequence comprising the polypeptide sequence of SEQ ID NO:2 and the polypeptide sequence of a selected targeting protein; and (b) a binding moiety that comprises an adapter protein, the adapter protein having a thiol group; the biological conjugate having a disulfide bond between the thiol group of SEQ ID NO:2 and the thiol group of the adapter protein. The present invention is also directed to biological sequences employed in the above biological conjugates, as well as pharmaceutical preparations and methods using the above biological conjugates.

Inventors:
 [1];  [1]
  1. W. Simsbury, CT
Issue Date:
Research Org.:
SibTech, Inc. (Newington, CT)
Sponsoring Org.:
USDOE
OSTI Identifier:
1014528
Patent Number(s):
7355019
Application Number:
11/083,508
Assignee:
SibTech, Inc. (Newington, CT)
Patent Classifications (CPCs):
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES
A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61P - SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
DOE Contract Number:  
FG02-02ER83520
Resource Type:
Patent
Country of Publication:
United States
Language:
English

Citation Formats

Backer, Marina V, and Backer, Joseph M. Cysteine-containing peptide tag for site-specific conjugation of proteins. United States: N. p., 2008. Web.
Backer, Marina V, & Backer, Joseph M. Cysteine-containing peptide tag for site-specific conjugation of proteins. United States.
Backer, Marina V, and Backer, Joseph M. Tue . "Cysteine-containing peptide tag for site-specific conjugation of proteins". United States. https://www.osti.gov/servlets/purl/1014528.
@article{osti_1014528,
title = {Cysteine-containing peptide tag for site-specific conjugation of proteins},
author = {Backer, Marina V and Backer, Joseph M},
abstractNote = {The present invention is directed to a biological conjugate, comprising: (a) a targeting moiety comprising a polypeptide having an amino acid sequence comprising the polypeptide sequence of SEQ ID NO:2 and the polypeptide sequence of a selected targeting protein; and (b) a binding moiety bound to the targeting moiety; the biological conjugate having a covalent bond between the thiol group of SEQ ID NO:2 and a functional group in the binding moiety. The present invention is directed to a biological conjugate, comprising: (a) a targeting moiety comprising a polypeptide having an amino acid sequence comprising the polypeptide sequence of SEQ ID NO:2 and the polypeptide sequence of a selected targeting protein; and (b) a binding moiety that comprises an adapter protein, the adapter protein having a thiol group; the biological conjugate having a disulfide bond between the thiol group of SEQ ID NO:2 and the thiol group of the adapter protein. The present invention is also directed to biological sequences employed in the above biological conjugates, as well as pharmaceutical preparations and methods using the above biological conjugates.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2008},
month = {4}
}

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