High resolution mass spectrometry method and system for analysis of whole proteins and other large molecules
Abstract
A matrix assisted laser desorption/ionization (MALDI) method and related system for analyzing high molecular weight analytes includes the steps of providing at least one matrix-containing particle inside an ion trap, wherein at least one high molecular weight analyte molecule is provided within the matrix-containing particle, and MALDI on the high molecular weight particle while within the ion trap. A laser power used for ionization is sufficient to completely vaporize the particle and form at least one high molecular weight analyte ion, but is low enough to avoid fragmenting the high molecular weight analyte ion. The high molecular weight analyte ion is extracted out from the ion trap, and is then analyzed using a detector. The detector is preferably a pyrolyzing and ionizing detector.
- Inventors:
-
- Knoxville, TN
- Naperville, IL
- Issue Date:
- Research Org.:
- Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1014525
- Patent Number(s):
- 7671330
- Application Number:
- US Patent Application 11/688,996
- Assignee:
- UT-Battelle, LLC (Oak Ridge, TN)
- Patent Classifications (CPCs):
-
H - ELECTRICITY H01 - BASIC ELECTRIC ELEMENTS H01J - ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
- DOE Contract Number:
- AC05-00OR22725
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
Citation Formats
Reilly, Peter T. A., and Harris, William A. High resolution mass spectrometry method and system for analysis of whole proteins and other large molecules. United States: N. p., 2010.
Web.
Reilly, Peter T. A., & Harris, William A. High resolution mass spectrometry method and system for analysis of whole proteins and other large molecules. United States.
Reilly, Peter T. A., and Harris, William A. Tue .
"High resolution mass spectrometry method and system for analysis of whole proteins and other large molecules". United States. https://www.osti.gov/servlets/purl/1014525.
@article{osti_1014525,
title = {High resolution mass spectrometry method and system for analysis of whole proteins and other large molecules},
author = {Reilly, Peter T. A. and Harris, William A},
abstractNote = {A matrix assisted laser desorption/ionization (MALDI) method and related system for analyzing high molecular weight analytes includes the steps of providing at least one matrix-containing particle inside an ion trap, wherein at least one high molecular weight analyte molecule is provided within the matrix-containing particle, and MALDI on the high molecular weight particle while within the ion trap. A laser power used for ionization is sufficient to completely vaporize the particle and form at least one high molecular weight analyte ion, but is low enough to avoid fragmenting the high molecular weight analyte ion. The high molecular weight analyte ion is extracted out from the ion trap, and is then analyzed using a detector. The detector is preferably a pyrolyzing and ionizing detector.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Mar 02 00:00:00 EST 2010},
month = {Tue Mar 02 00:00:00 EST 2010}
}
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Works referenced in this record:
Aerosol MALDI of peptides and proteins in an ion trap mass spectrometer: Trapping, resolution and signal-to-noise
journal, December 2006
- Harris, William A.; Reilly, Peter T. A.; Whitten, William B.
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MALDI of Individual Biomolecule-Containing Airborne Particles in an Ion Trap Mass Spectrometer
journal, July 2005
- Harris, William A.; Reilly, Peter T. A.; Whitten, William B.
- Analytical Chemistry, Vol. 77, Issue 13
A digital ion trap mass spectrometer coupled with atmospheric pressure ion sources
journal, May 2004
- Ding, Li; Sudakov, Michael; Brancia, Francesco L.
- Journal of Mass Spectrometry, Vol. 39, Issue 5
Laser desorption ionization of proteins with molecular masses exceeding 10,000 daltons
journal, October 1988
- Karas, Michael.; Hillenkamp, Franz.
- Analytical Chemistry, Vol. 60, Issue 20