3-dimensional imaging at nanometer resolutions
Abstract
An apparatus and method for enabling precise, 3-dimensional, photoactivation localization microscopy (PALM) using selective, two-photon activation of fluorophores in a single z-slice of a sample in cooperation with time-gated imaging for reducing the background radiation from other image planes to levels suitable for single-molecule detection and spatial location, are described.
- Inventors:
-
- Los Alamos, NM
- Santa Fe, NM
- Issue Date:
- Research Org.:
- Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1014498
- Patent Number(s):
- 7675045
- Application Number:
- U S Patent Application 12/248,834
- Assignee:
- Los Alamos National Security, LLC (Los Alamos, NM)
- Patent Classifications (CPCs):
-
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
G - PHYSICS G02 - OPTICS G02B - OPTICAL ELEMENTS, SYSTEMS, OR APPARATUS
- DOE Contract Number:
- AC52-06NA25396
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
Citation Formats
Werner, James H, Goodwin, Peter M, and Shreve, Andrew P. 3-dimensional imaging at nanometer resolutions. United States: N. p., 2010.
Web.
Werner, James H, Goodwin, Peter M, & Shreve, Andrew P. 3-dimensional imaging at nanometer resolutions. United States.
Werner, James H, Goodwin, Peter M, and Shreve, Andrew P. Tue .
"3-dimensional imaging at nanometer resolutions". United States. https://www.osti.gov/servlets/purl/1014498.
@article{osti_1014498,
title = {3-dimensional imaging at nanometer resolutions},
author = {Werner, James H and Goodwin, Peter M and Shreve, Andrew P},
abstractNote = {An apparatus and method for enabling precise, 3-dimensional, photoactivation localization microscopy (PALM) using selective, two-photon activation of fluorophores in a single z-slice of a sample in cooperation with time-gated imaging for reducing the background radiation from other image planes to levels suitable for single-molecule detection and spatial location, are described.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2010},
month = {3}
}
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Works referenced in this record:
Tracking of single fluorescent particles in three dimensions: use of cylindrical optics to encode particle position
journal, September 1994
- Kao, H. P.; Verkman, A. S.
- Biophysical Journal, Vol. 67, Issue 3, p. 1291-1300
Three-Dimensional Super-Resolution Imaging by Stochastic Optical Reconstruction Microscopy
journal, February 2008
- Huang, B.; Wang, W.; Bates, M.
- Science, Vol. 319, Issue 5864
Two-photon laser scanning fluorescence microscopy
journal, April 1990
- Denk, W.; Strickler, J.; Webb, W.
- Science, Vol. 248, Issue 4951
Two-Photon Activation and Excitation Properties of PA-GFP in the 720–920-nm Region
journal, August 2005
- Schneider, Marc; Barozzi, Sara; Testa, Ilaria
- Biophysical Journal, Vol. 89, Issue 2
Imaging Intracellular Fluorescent Proteins at Nanometer Resolution
journal, September 2006
- Betzig, E.; Patterson, G. H.; Sougrat, R.
- Science, Vol. 313, Issue 5793
Three-dimensional tracking of individual quantum dots
journal, November 2007
- Lessard, Guillaume A.; Goodwin, Peter M.; Werner, James H.
- Applied Physics Letters, Vol. 91, Issue 22
Polewards microtubule flux in the mitotic spindle: evidence from photoactivation of fluorescence.
journal, August 1989
- Mitchison, T. J.
- The Journal of Cell Biology, Vol. 109, Issue 2
Time-gated biological imaging by use of colloidal quantum dots
journal, January 2001
- Dahan, M.; Laurence, T.; Pinaud, F.
- Optics Letters, Vol. 26, Issue 11