Insulator-based DEP with impedance measurements for analyte detection

Davalos, Rafael V; Simmons, Blake A; Crocker, Robert W; Cummings, Eric B

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Title: Insulator-based DEP with impedance measurements for analyte detection

Abstract

Disclosed herein are microfluidic devices for assaying at least one analyte specie in a sample comprising at least one analyte concentration area in a microchannel having insulating structures on or in at least one wall of the microchannel which provide a nonuniform electric field in the presence of an electric field provided by off-chip electrodes; and a pair of passivated sensing electrodes for impedance detection in a detection area. Also disclosed are assay methods and methods of making.

Inventors:

Davalos, Rafael V ^[1]; Simmons, Blake A ^[2]; Crocker, Robert W ^[3]; Cummings, Eric B ^[4]

Blacksburg, VA
San Francisco, CA
Fremont, CA
Livermore, CA

Issue Date:: 2010-03-16

Research Org.:: Sandia Corporation (Livermore, CA)

Sponsoring Org.:: USDOE

OSTI Identifier:: 1014491

Patent Number(s):: 7678256

Application Number:: US Patent Application 11/556,685

Assignee:: Sandia Corporation (Livermore, CA)

Patent Classifications (CPCs):: B - PERFORMING OPERATIONS B03 - SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS B03C - MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS

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B - PERFORMING OPERATIONS B03 - SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS B03C - MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS
B03C2201/24 - for measuring or calculating parameters, efficiency, etc.
B03C2201/26 - for use in medical applications
B03C5/005 - {Dielectrophoresis, i.e. dielectric particles migrating towards the region of highest field strength
B03C5/026 - {using open-gradient differential dielectric separation, i.e. using electrodes of special shapes for non-uniform field creation, e.g. Fluid Integrated Circuit [FIC]}

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DOE Contract Number:: AC04-94AL85000

Resource Type:: Patent

Country of Publication:: United States

Language:: English

Citation Formats


                    Davalos, Rafael V, Simmons, Blake A, Crocker, Robert W, and Cummings, Eric B. Insulator-based DEP with impedance measurements for analyte detection.  United States: N. p., 2010. 
        Web.

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                    Davalos, Rafael V, Simmons, Blake A, Crocker, Robert W, & Cummings, Eric B. Insulator-based DEP with impedance measurements for analyte detection.  United States.

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                    Davalos, Rafael V, Simmons, Blake A, Crocker, Robert W, and Cummings, Eric B. Tue .  
        "Insulator-based DEP with impedance measurements for analyte detection".  United States.  https://www.osti.gov/servlets/purl/1014491.

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@article{osti_1014491,

  title        = {Insulator-based DEP with impedance measurements for analyte detection},

  author       = {Davalos, Rafael V and Simmons, Blake A and Crocker, Robert W and Cummings, Eric B},

  abstractNote = {Disclosed herein are microfluidic devices for assaying at least one analyte specie in a sample comprising at least one analyte concentration area in a microchannel having insulating structures on or in at least one wall of the microchannel which provide a nonuniform electric field in the presence of an electric field provided by off-chip electrodes; and a pair of passivated sensing electrodes for impedance detection in a detection area. Also disclosed are assay methods and methods of making.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {2010},

  month        = {3}

}

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Works referenced in this record:

Separation of viable and non-viable yeast using dielectrophoresis
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Markx, Gerard H.; Talary, Mark S.; Pethig, Ronald
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Dielectrophoretic Concentration and Separation of Live and Dead Bacteria in an Array of Insulators
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Lapizco-Encinas, Blanca H.; Simmons, Blake A.; Cummings, Eric B.
Analytical Chemistry, Vol. 76, Issue 6
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Effective Conductivity of a Suspension of Permeabilized Cells: A Theoretical Analysis
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Electrostatic manipulation of DNA in microfabricated structures
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Selective detection of specific bacteria using dielectrophoretic impedance measurement method combined with an antigen–antibody reaction
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Suehiro, Junya; Noutomi, Daisuke; Shutou, Masanori
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Dielectric properties of native and decoated spores of Bacillus megaterium.
journal, January 1979

Carstensen, E. L.; Marquis, R. E.; Child, S. Z.
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Title: Insulator-based DEP with impedance measurements for analyte detection

Abstract

Citation Formats

Separation of viable and non-viable yeast using dielectrophoresis journal, January 1994

An insulator-based (electrodeless) dielectrophoretic concentrator for microbes in water journal, September 2005

Dielectrophoretic Concentration and Separation of Live and Dead Bacteria in an Array of Insulators journal, March 2004

Dielectrophoresis in Microchips Containing Arrays of Insulating Posts: Theoretical and Experimental Results journal, September 2003

Cell Separation on Microfabricated Electrodes Using Dielectrophoretic/Gravitational Field-Flow Fractionation journal, March 1999

Effective Conductivity of a Suspension of Permeabilized Cells: A Theoretical Analysis journal, August 2003

Electrostatic manipulation of DNA in microfabricated structures journal, January 1990

High sensitive detection of biological cells using dielectrophoretic impedance measurement method combined with electropermeabilization journal, November 2003

Selective detection of viable bacteria using dielectrophoretic impedance measurement method journal, February 2003

Selective detection of specific bacteria using dielectrophoretic impedance measurement method combined with an antigen–antibody reaction journal, June 2003

Dielectric properties of native and decoated spores of Bacillus megaterium. journal, January 1979

Real-Time Virus Trapping and Fluorescent Imaging in Microfluidic Devices journal, February 2004

Electrical impedance-spectroscopy particle detector for use in microanalysis systems conference, August 1999

Electronic manipulation of DNA, proteins, and nanoparticles for potential circuit assembly journal, October 2004

Polymeric microfluidic devices for the monitoring and separation of water-borne pathogens utilizing insulative dielectrophoresis conference, January 2005

Insulator-based dielectrophoresis for the selective concentration and separation of live bacteria in water journal, June 2004

Separation of viable and non-viable yeast using dielectrophoresis
journal, January 1994

An insulator-based (electrodeless) dielectrophoretic concentrator for microbes in water
journal, September 2005

Dielectrophoretic Concentration and Separation of Live and Dead Bacteria in an Array of Insulators
journal, March 2004

Dielectrophoresis in Microchips Containing Arrays of Insulating Posts: Theoretical and Experimental Results
journal, September 2003

Cell Separation on Microfabricated Electrodes Using Dielectrophoretic/Gravitational Field-Flow Fractionation
journal, March 1999

Effective Conductivity of a Suspension of Permeabilized Cells: A Theoretical Analysis
journal, August 2003

Electrostatic manipulation of DNA in microfabricated structures
journal, January 1990

High sensitive detection of biological cells using dielectrophoretic impedance measurement method combined with electropermeabilization
journal, November 2003

Selective detection of viable bacteria using dielectrophoretic impedance measurement method
journal, February 2003

Selective detection of specific bacteria using dielectrophoretic impedance measurement method combined with an antigen–antibody reaction
journal, June 2003

Dielectric properties of native and decoated spores of Bacillus megaterium.
journal, January 1979

Real-Time Virus Trapping and Fluorescent Imaging in Microfluidic Devices
journal, February 2004

Electrical impedance-spectroscopy particle detector for use in microanalysis systems
conference, August 1999

Electronic manipulation of DNA, proteins, and nanoparticles for potential circuit assembly
journal, October 2004

Polymeric microfluidic devices for the monitoring and separation of water-borne pathogens utilizing insulative dielectrophoresis
conference, January 2005

Insulator-based dielectrophoresis for the selective concentration and separation of live bacteria in water
journal, June 2004