Robust expression of a bioactive mammalian protein in chlamydomonas chloroplast

Mayfield, Stephen P

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Title: Robust expression of a bioactive mammalian protein in chlamydomonas chloroplast

Abstract

Methods and compositions are disclosed to engineer chloroplast comprising heterologous mammalian genes via a direct replacement of chloroplast Photosystem II (PSII) reaction center protein coding regions to achieve expression of recombinant protein above 5% of total protein. When algae is used, algal expressed protein is produced predominantly as a soluble protein where the functional activity of the peptide is intact. As the host algae is edible, production of biologics in this organism for oral delivery or proteins/peptides, especially gut active proteins, without purification is disclosed.

Inventors:

Mayfield, Stephen P ^[1]

Cardiff, CA

Issue Date:: Tue Mar 16 00:00:00 EDT 2010

Research Org.:: The Scripps Research Inst., La Jolla, CA (United States)

Sponsoring Org.:: USDOE

OSTI Identifier:: 1014485

Patent Number(s):: 7678561

Application Number:: US Patent Application 11/801,506

Assignee:: The Scripps Research Institute (La Jolla, CA)

Patent Classifications (CPCs):: A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61P - SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

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A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61P - SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
A61P31/00 - Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C12N15/79 - Vectors or expression systems specially adapted for eukaryotic hosts
C12N15/8214 - {Plastid transformation}
C12N15/8257 - {for the production of primary gene products, e.g. pharmaceutical products, interferon}

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DOE Contract Number:: FG03-02ER15313

Resource Type:: Patent

Country of Publication:: United States

Language:: English

Citation Formats


                    Mayfield, Stephen P. Robust expression of a bioactive mammalian protein in chlamydomonas chloroplast.  United States: N. p., 2010. 
        Web.

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                    Mayfield, Stephen P. Robust expression of a bioactive mammalian protein in chlamydomonas chloroplast.  United States.

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                    Mayfield, Stephen P. Tue .  
        "Robust expression of a bioactive mammalian protein in chlamydomonas chloroplast".  United States.  https://www.osti.gov/servlets/purl/1014485.

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@article{osti_1014485,

  title        = {Robust expression of a bioactive mammalian protein in chlamydomonas chloroplast},

  author       = {Mayfield, Stephen P},

  abstractNote = {Methods and compositions are disclosed to engineer chloroplast comprising heterologous mammalian genes via a direct replacement of chloroplast Photosystem II (PSII) reaction center protein coding regions to achieve expression of recombinant protein above 5% of total protein. When algae is used, algal expressed protein is produced predominantly as a soluble protein where the functional activity of the peptide is intact. As the host algae is edible, production of biologics in this organism for oral delivery or proteins/peptides, especially gut active proteins, without purification is disclosed.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {Tue Mar 16 00:00:00 EDT 2010},

  month        = {Tue Mar 16 00:00:00 EDT 2010}

}

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Works referenced in this record:

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Maul, Jude E.; Lilly, Jason W.; Cui, Liying
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Chloroplast DNA codon use: Evidence for selection at the psb A locus based on tRNA availability
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Morton, BrianR.
Journal of Molecular Evolution, Vol. 37, Issue 3
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