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Title: Robust expression of a bioactive mammalian protein in chlamydomonas chloroplast

Abstract

Methods and compositions are disclosed to engineer chloroplast comprising heterologous mammalian genes via a direct replacement of chloroplast Photosystem II (PSII) reaction center protein coding regions to achieve expression of recombinant protein above 5% of total protein. When algae is used, algal expressed protein is produced predominantly as a soluble protein where the functional activity of the peptide is intact. As the host algae is edible, production of biologics in this organism for oral delivery or proteins/peptides, especially gut active proteins, without purification is disclosed.

Inventors:
 [1]
  1. Cardiff, CA
Issue Date:
Research Org.:
The Scripps Research Inst., La Jolla, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1014485
Patent Number(s):
7678561
Application Number:
US Patent Application 11/801,506
Assignee:
The Scripps Research Institute (La Jolla, CA)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61P - SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
DOE Contract Number:  
FG03-02ER15313
Resource Type:
Patent
Country of Publication:
United States
Language:
English

Citation Formats

Mayfield, Stephen P. Robust expression of a bioactive mammalian protein in chlamydomonas chloroplast. United States: N. p., 2010. Web.
Mayfield, Stephen P. Robust expression of a bioactive mammalian protein in chlamydomonas chloroplast. United States.
Mayfield, Stephen P. Tue . "Robust expression of a bioactive mammalian protein in chlamydomonas chloroplast". United States. https://www.osti.gov/servlets/purl/1014485.
@article{osti_1014485,
title = {Robust expression of a bioactive mammalian protein in chlamydomonas chloroplast},
author = {Mayfield, Stephen P},
abstractNote = {Methods and compositions are disclosed to engineer chloroplast comprising heterologous mammalian genes via a direct replacement of chloroplast Photosystem II (PSII) reaction center protein coding regions to achieve expression of recombinant protein above 5% of total protein. When algae is used, algal expressed protein is produced predominantly as a soluble protein where the functional activity of the peptide is intact. As the host algae is edible, production of biologics in this organism for oral delivery or proteins/peptides, especially gut active proteins, without purification is disclosed.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2010},
month = {3}
}

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