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Title: Methods and devices for high-throughput dielectrophoretic concentration

Abstract

Disclosed herein are methods and devices for assaying and concentrating analytes in a fluid sample using dielectrophoresis. As disclosed, the methods and devices utilize substrates having a plurality of pores through which analytes can be selectively prevented from passing, or inhibited, on application of an appropriate electric field waveform. The pores of the substrate produce nonuniform electric field having local extrema located near the pores. These nonuniform fields drive dielectrophoresis, which produces the inhibition. Arrangements of electrodes and porous substrates support continuous, bulk, multi-dimensional, and staged selective concentration.

Inventors:
 [1];  [2];  [3];  [2];  [4];  [5]
  1. San Francisco, CA
  2. Livermore, CA
  3. Germantown, MD
  4. Ann Arbor, MI
  5. Escalon, CA
Issue Date:
Research Org.:
Sandia Corporation (Livermore, CA)
Sponsoring Org.:
USDOE
OSTI Identifier:
1014186
Patent Number(s):
7666289
Application Number:
US Patent Application 12/202,489
Assignee:
Sandia Corporation (Livermore, CA)
Patent Classifications (CPCs):
Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y10 - TECHNICAL SUBJECTS COVERED BY FORMER USPC Y10T - TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01D - SEPARATION
DOE Contract Number:  
AC04-94AL85000
Resource Type:
Patent
Country of Publication:
United States
Language:
English

Citation Formats

Simmons, Blake A, Cummings, Eric B, Fiechtner, Gregory J, Fintschenko, Yolanda, McGraw, Gregory J, and Salmi, Allen. Methods and devices for high-throughput dielectrophoretic concentration. United States: N. p., 2010. Web.
Simmons, Blake A, Cummings, Eric B, Fiechtner, Gregory J, Fintschenko, Yolanda, McGraw, Gregory J, & Salmi, Allen. Methods and devices for high-throughput dielectrophoretic concentration. United States.
Simmons, Blake A, Cummings, Eric B, Fiechtner, Gregory J, Fintschenko, Yolanda, McGraw, Gregory J, and Salmi, Allen. Tue . "Methods and devices for high-throughput dielectrophoretic concentration". United States. https://www.osti.gov/servlets/purl/1014186.
@article{osti_1014186,
title = {Methods and devices for high-throughput dielectrophoretic concentration},
author = {Simmons, Blake A and Cummings, Eric B and Fiechtner, Gregory J and Fintschenko, Yolanda and McGraw, Gregory J and Salmi, Allen},
abstractNote = {Disclosed herein are methods and devices for assaying and concentrating analytes in a fluid sample using dielectrophoresis. As disclosed, the methods and devices utilize substrates having a plurality of pores through which analytes can be selectively prevented from passing, or inhibited, on application of an appropriate electric field waveform. The pores of the substrate produce nonuniform electric field having local extrema located near the pores. These nonuniform fields drive dielectrophoresis, which produces the inhibition. Arrangements of electrodes and porous substrates support continuous, bulk, multi-dimensional, and staged selective concentration.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2010},
month = {2}
}

Works referenced in this record:

Dielectrophoretic Concentration and Separation of Live and Dead Bacteria in an Array of Insulators
journal, March 2004


The Motion and Precipitation of Suspensoids in Divergent Electric Fields
journal, July 1951


Manipulation of herpes simplex virus type 1 by dielectrophoresis
journal, September 1998


Dielectrophoresis in Microchips Containing Arrays of Insulating Posts:  Theoretical and Experimental Results
journal, September 2003


Cell Separation on Microfabricated Electrodes Using Dielectrophoretic/Gravitational Field-Flow Fractionation
journal, March 1999


Introducing dielectrophoresis as a new force field for field-flow fractionation
journal, August 1997


Insulator-based dielectrophoresis for the selective concentration and separation of live bacteria in water
journal, June 2004