Expanding the eukaryotic genetic code

Chin, Jason W; Cropp, T Ashton; Anderson, J Christopher; Schultz, Peter G

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Title: Expanding the eukaryotic genetic code

Abstract

This invention provides compositions and methods for producing translational components that expand the number of genetically encoded amino acids in eukaryotic cells. The components include orthogonal tRNAs, orthogonal aminoacyl-tRNA synthetases, orthogonal pairs of tRNAs/synthetases and unnatural amino acids. Proteins and methods of producing proteins with unnatural amino acids in eukaryotic cells are also provided.

Inventors:

Chin, Jason W ^[1]; Cropp, T Ashton ^[2]; Anderson, J Christopher ^[3]; Schultz, Peter G ^[4]

Cambridge, GB
Bethesda, MD
San Francisco, CA
La Jolla, CA

Issue Date:: 2009-12-01

Research Org.:: The Scripps Research Inst., La Jolla, CA (United States)

Sponsoring Org.:: USDOE

OSTI Identifier:: 1014015

Patent Number(s):: 7625717

Application Number:: US Patent Application 11/710,655

Assignee:: The Scripps Research Institute (La Jolla, CA)

Patent Classifications (CPCs):: A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61K - PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES

A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61P - SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS

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A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61K - PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
A61K38/1709 - {from mammals}

A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61P - SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
A61P43/00 - Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C12N15/67 - General methods for enhancing the expression
C12N15/81 - for yeasts
C12N9/93 - {Ligases

C - CHEMISTRY C12 - BIOCHEMISTRY C12Y - ENZYMES
C12Y601/01 - Ligases forming aminoacyl-tRNA and related compounds
C12Y601/01001 - Tyrosine-tRNA ligase
C12Y601/0102 - Phenylalanine-tRNA ligase

Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y10 - TECHNICAL SUBJECTS COVERED BY FORMER USPC Y10S - TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
Y10S435/81 - Packaged device or kit
Y10S435/975 - Kit

Show less

DOE Contract Number:: FG03-00ER45812

Resource Type:: Patent

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES

Citation Formats


                    Chin, Jason W, Cropp, T Ashton, Anderson, J Christopher, and Schultz, Peter G. Expanding the eukaryotic genetic code.  United States: N. p., 2009. 
        Web.

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                    Chin, Jason W, Cropp, T Ashton, Anderson, J Christopher, & Schultz, Peter G. Expanding the eukaryotic genetic code.  United States.

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                    Chin, Jason W, Cropp, T Ashton, Anderson, J Christopher, and Schultz, Peter G. Tue .  
        "Expanding the eukaryotic genetic code".  United States.  https://www.osti.gov/servlets/purl/1014015.

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@article{osti_1014015,

  title        = {Expanding the eukaryotic genetic code},

  author       = {Chin, Jason W and Cropp, T Ashton and Anderson, J Christopher and Schultz, Peter G},

  abstractNote = {This invention provides compositions and methods for producing translational components that expand the number of genetically encoded amino acids in eukaryotic cells. The components include orthogonal tRNAs, orthogonal aminoacyl-tRNA synthetases, orthogonal pairs of tRNAs/synthetases and unnatural amino acids. Proteins and methods of producing proteins with unnatural amino acids in eukaryotic cells are also provided.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {2009},

  month        = {12}

}

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Works referenced in this record:

Engineering Chemical Reactivity on Cell Surfaces Through Oligosaccharide Biosynthesis
journal, May 1997

Mahal, Lara K.; Yarema, Kevin J.; Bertozzi, Carolyn R.
Science, Vol. 276, Issue 5315, p. 1125-1128
https://doi.org/10.1126/science.276.5315.1125

Site-Specific Protein Modification Using a Ketone Handle
journal, January 1996

Cornish, Virginia W.; Hahn, Klaus M.; Schultz, Peter G.
Journal of the American Chemical Society, Vol. 118, Issue 34, p. 8150-8151
https://doi.org/10.1021/ja961216x

Incorporation of azides into recombinant proteins for chemoselective modification by the Staudinger ligation
journal, December 2001

Kiick, K. L.; Saxon, E.; Tirrell, D. A.
Proceedings of the National Academy of Sciences, Vol. 99, Issue 1, p. 19-24
https://doi.org/10.1073/pnas.012583299

Bioconjugation by Copper(I)-Catalyzed Azide-Alkyne [3 + 2] Cycloaddition
journal, March 2003

Wang, Qian; Chan, Timothy R.; Hilgraf, Robert
Journal of the American Chemical Society, Vol. 125, Issue 11, p. 3192-3193
https://doi.org/10.1021/ja021381e

An engineered Escherichia coli tyrosyl-tRNA synthetase for site-specific incorporation of an unnatural amino acid into proteins in eukaryotic translation and its application in a wheat germ cell-free system
journal, July 2002

Kiga, D.; Sakamoto, K.; Kodama, K.
Proceedings of the National Academy of Sciences, Vol. 99, Issue 15, p. 9715-9720
https://doi.org/10.1073/pnas.142220099

Addition of p-Azido-L-phenylalanine to the Genetic Code of Escherichia coli
journal, July 2002

Chin, Jason W.; Santoro, Stephen W.; Martin, Andrew B.
Journal of the American Chemical Society, Vol. 124, Issue 31, p. 9026-9027
https://doi.org/10.1021/ja027007w

An Expanded Eukaryotic Genetic Code
journal, August 2003

Chin, Jason W.; Cropp, T. Ashton; Anderson, J. Christopher
Science, Vol. 301, Issue 5635, p. 964-967
https://doi.org/10.1126/science.1084772

A New Strategy for the Site-Specific Modification of Proteins in Vivo
journal, June 2003

Zhang, Zhiwen; Smith, Brian A. C.; Wang, Lei
Biochemistry, Vol. 42, Issue 22, p. 6735-6746
https://doi.org/10.1021/bi0300231

Site-specific incorporation of biotinylated amino acids to identify surface-exposed residues in integral membrane proteins
journal, October 1997

Gallivan, Justin P.; Lester, Henry A.; Dougherty, Dennis A.
Chemistry & Biology, Vol. 4, Issue 10, p. 739-749
https://doi.org/10.1016/S1074-5521(97)90312-4

Misacylation of Yeast Amber Suppressor tRNATyr by E. coli Lysyl-tRNA Synthetase and Its Effective Repression by Genetic Engineering of the tRNA Sequence
journal, April 2006

Fukunaga, Jun-ichi; Yokogawa, Takashi; Ohno, Satoshi
The Journal of Biochemistry, Vol. 139, Issue 4
https://doi.org/10.1093/jb/mvj078

Chemoselective ligation reactions with proteins, oligosaccharides and cells
journal, December 1998

Lemieux, George A.; Bertozzi, Carolyn R.
Trends in Biotechnology, Vol. 16, Issue 12, p. 506-513
https://doi.org/10.1016/S0167-7799(98)01230-X

Intermolecular 1,3-Dipolar Cycloadditions
book, January 1991

Padwa, Albert
Comprehensive Organic Synthesis
https://doi.org/10.1016/B978-0-08-052349-1.00116-5

Aliphatic amino azides as key building blocks for efficient polyamine syntheses
journal, July 1993

Carboni, Bertrand; Benalil, Aziza; Vaultier, Michel
The Journal of Organic Chemistry, Vol. 58, Issue 14
https://doi.org/10.1021/jo00066a028

Partition of tRNA synthetases into two classes based on mutually exclusive sets of sequence motifs
journal, September 1990

Eriani, Gilbert; Delarue, Marc; Poch, Olivier
Nature, Vol. 347, Issue 6289, p. 203-206
https://doi.org/10.1038/347203a0

Adding l-3-(2-Naphthyl)alanine to the Genetic Code of E. coli
journal, March 2002

Wang, Lei; Brock, Ansgar; Schultz, Peter G.
Journal of the American Chemical Society, Vol. 124, Issue 9, p. 1836-1837
https://doi.org/10.1021/ja012307j

Recent advances in technology for measuring and manipulating cell signals
journal, June 2000

Zacharias, David A.; Baird, Geoffrey S.; Tsien, Roger Y.
Current Opinion in Neurobiology, Vol. 10, Issue 3, p. 416-421
https://doi.org/10.1016/S0959-4388(00)00101-X

Expanding tRNA recognition of a tRNA synthetase by a single amino acid change
journal, May 2003

Feng, L.; Tumbula-Hansen, D.; Toogood, H.
Proceedings of the National Academy of Sciences, Vol. 100, Issue 10, p. 5676-5681
https://doi.org/10.1073/pnas.0631525100

Addition of the keto functional group to the genetic code of Escherichia coli
journal, December 2002

Wang, L.; Zhang, Z.; Brock, A.
Proceedings of the National Academy of Sciences, Vol. 100, Issue 1, p. 56-61
https://doi.org/10.1073/pnas.0234824100

Unnatural amino acids as probes of protein structure and function
journal, December 2000

Dougherty, D.
Current Opinion in Chemical Biology, Vol. 4, Issue 6, p. 645-652
https://doi.org/10.1016/S1367-5931(00)00148-4

Progress toward the evolution of an organism with an expanded genetic code
journal, April 1999

Liu, David R.; Schultz, Peter G.
Proceedings of the National Academy of Sciences, Vol. 96, Issue 9, p. 4780-4785
https://doi.org/10.1073/pnas.96.9.4780

Decomposition of Protein Tryptophan Fluorescence Spectra into Log-Normal Components. II. The Statistical Proof of Discreteness of Tryptophan Classes in Proteins
journal, September 2001

Reshetnyak, Yana K.; Burstein, Edward A.
Biophysical Journal, Vol. 81, Issue 3, p. 1710-1734
https://doi.org/10.1016/S0006-3495(01)75824-9

Specific Covalent Labeling of Recombinant Protein Molecules Inside Live Cells
journal, July 1998

Griffin, B. Albert; Adams, Stephen R.; Tsien, Roger Y.
Science, Vol. 281, Issue 5374, p. 269-272
https://doi.org/10.1126/science.281.5374.269

Exploring the Limits of Codon and Anticodon Size
journal, February 2002

Anderson, J. Christopher; Magliery, Thomas J.; Schultz, Peter G.
Chemistry & Biology, Vol. 9, Issue 2, p. 237-244
https://doi.org/10.1016/S1074-5521(02)00094-7

Protein Tyrosine Kinase Csk-Catalyzed Phosphorylation of Src Containing Unnatural Tyrosine Analogues
journal, September 2001

Wang, Dongxia; Cole, Philip A.
Journal of the American Chemical Society, Vol. 123, Issue 37, p. 8883-8886
https://doi.org/10.1021/ja010540b

Regulated phosphorylation and dephosphorylation of GAL4, a transcriptional activator.
journal, August 1989

Mylin, L. M.; Bhat, J. P.; Hopper, J. E.
Genes & Development, Vol. 3, Issue 8, p. 1157-1165
https://doi.org/10.1101/gad.3.8.1157

Activity-Based Protein Profiling in Vivo Using a Copper(I)-Catalyzed Azide-Alkyne [3 + 2] Cycloaddition
journal, April 2003

Speers, Anna E.; Adam, Gregory C.; Cravatt, Benjamin F.
Journal of the American Chemical Society, Vol. 125, Issue 16, p. 4686-4687
https://doi.org/10.1021/ja034490h

Peptidotriazoles on Solid Phase [1,2,3]-Triazoles by Regiospecific Copper(I)-Catalyzed 1,3-Dipolar Cycloadditions of Terminal Alkynes to Azides
journal, May 2002

Tornøe, Christian W.; Christensen, Caspar; Meldal, Morten
The Journal of Organic Chemistry, Vol. 67, Issue 9, p. 3057-3064
https://doi.org/10.1021/jo011148j

Addition of a photocrosslinking amino acid to the genetic code of Escherichia coli
journal, August 2002

Chin, J. W.; Martin, A. B.; King, D. S.
Proceedings of the National Academy of Sciences, Vol. 99, Issue 17, p. 11020-11024
https://doi.org/10.1073/pnas.172226299

Expanding the genetic code: selection of efficient suppressors of four-base codons and identification of “shifty” four-base codons with a library approach in Escherichia coli
journal, March 2001

Magliery, Thomas J.; Anderson, J. Christopher; Schultz, Peter G.
Journal of Molecular Biology, Vol. 307, Issue 3, p. 755-769
https://doi.org/10.1006/jmbi.2001.4518

Aminoacyl-tRNA synthetases: Versatile players in the changing theater of translation
journal, November 2002

Francklyn, Christopher; Perona, John J.; Puetz, Joern
RNA, Vol. 8, Issue 11
https://doi.org/10.1017/S1355838202021180

Imaging biochemistry inside cells
journal, May 2001

Wouters, Fred S.; Verveer, Peter J.; Bastiaens, Philippe I.H.
Trends in Cell Biology, Vol. 11, Issue 5, p. 203-211
https://doi.org/10.1016/S0962-8924(01)01982-1

Preparation of biological labels with acetylenic linker arms
journal, January 1997

Crisp, Geoffrey T.; Gore, Jeffrey
Tetrahedron, Vol. 53, Issue 4, p. 1505-1522
https://doi.org/10.1016/S0040-4020(96)01084-8

A Mutant Escherichia coli Tyrosyl-tRNA Synthetase Utilizes the Unnatural Amino Acid Azatyrosine More Efficiently than Tyrosine
journal, September 2000

Hamano-Takaku, Fumie; Iwama, Toshiharu; Saito-Yano, Shio
Journal of Biological Chemistry, Vol. 275, Issue 51, p. 40324-40328
https://doi.org/10.1074/jbc.M003696200

Adding Amino Acids with Novel Reactivity to the Genetic Code of Saccharomyces Cerevisiae
journal, October 2003

Deiters, Alexander; Cropp, T. Ashton; Mukherji, Mridul
Journal of the American Chemical Society, Vol. 125, Issue 39, p. 11782-11783
https://doi.org/10.1021/ja0370037

Expanding the genetic code
journal, December 2001

Wang, Lei; Schultz, Peter G.
Chemical Communications, Issue 1
https://doi.org/10.1039/b108185n

An expanding genetic code
journal, December 2004

Cropp, T. Ashton; Schultz, Peter G.
Trends in Genetics, Vol. 20, Issue 12, p. 625-630
https://doi.org/10.1016/j.tig.2004.09.013

Progress Toward an Expanded Eukaryotic Genetic Code
journal, June 2003

Chin, Jason W.; Cropp, T. Ashton; Chu, Stephanie
Chemistry & Biology, Vol. 10, Issue 6, p. 511-519
https://doi.org/10.1016/S1074-5521(03)00123-6

Cellular screening assays using fluorescence microscopy
journal, October 2001

Blake, R.
Current Opinion in Pharmacology, Vol. 1, Issue 5, p. 533-539
https://doi.org/10.1016/S1471-4892(01)00092-3

Programming peptidomimetic syntheses by translating genetic codes designed de novo
journal, May 2003

Forster, A. C.; Tan, Z.; Nalam, M. N. L.
Proceedings of the National Academy of Sciences, Vol. 100, Issue 11, p. 6353-6357
https://doi.org/10.1073/pnas.1132122100

A bacterial amber suppressor in Saccharomyces cerevisiae is selectively recognized by a bacterial aminoacyl-tRNA synthetase.
journal, April 1990

Edwards, H.; Schimmel, P.
Molecular and Cellular Biology, Vol. 10, Issue 4, p. 1633-1641
https://doi.org/10.1128/MCB.10.4.1633

Expanding the Genetic Code of Escherichia coli
journal, April 2001

Wang, L.; Brock, A.; Herberich, B.
Science, Vol. 292, Issue 5516, p. 498-500
https://doi.org/10.1126/science.1060077

Synthesis and Photophysical Properties of Fluorescent Derivatives of Methylmercury
journal, January 1996

Bolletta, Fabrizio; Fabbri, Daniele; Lombardo, Marco
Organometallics, Vol. 15, Issue 9, p. 2415-2417
https://doi.org/10.1021/om950793b

Site-specific incorporation of an unnatural amino acid into proteins in mammalian cells
journal, November 2002

Sakamoto, K.
Nucleic Acids Research, Vol. 30, Issue 21, p. 4692-4699
https://doi.org/10.1093/nar/gkf589

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Expanding the eukaryotic genetic code

Patent Chin, Jason W.; Cropp, T. Ashton; Anderson, J. Christopher; ...

This invention provides compositions and methods for producing translational components that expand the number of genetically encoded amino acids in eukaryotic cells. The components include orthogonal tRNAs, orthogonal aminoacyl-tRNA synthetases, orthogonal pairs of tRNAs/synthetases and unnatural amino acids. Proteins and methods of producing proteins with unnatural amino acids in eukaryotic cells are also provided.
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Expanding the eukaryotic genetic code

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This invention provides compositions and methods for producing translational components that expand the number of genetically encoded amino acids in eukaryotic cells. The components include orthogonal tRNAs, orthogonal aminoacyl-tRNA synthetases, orthogonal pairs of tRNAs/synthetases and unnatural amino acids. Proteins and methods of producing proteins with unnatural amino acids in eukaryotic cells are also provided.
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This invention provides compositions and methods for producing translational components that expand the number of genetically encoded amino acids in eukaryotic cells. The components include orthogonal tRNAs, orthogonal aminoacyl-tRNA synthetases, orthogonal pairs of tRNAs/synthetases and unnatural amino acids. Proteins and methods of producing proteins with unnatural amino acids in eukaryotic cells are also provided.
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This invention provides compositions and methods for producing translational components that expand the number of genetically encoded amino acids in eukaryotic cells. The components include orthogonal tRNAs, orthogonal aminoacyl-tRNA synthetases, orthogonal pairs of tRNAs/synthetases and unnatural amino acids. Proteins and methods of producing proteins with unnatural amino acids in eukaryotic cells are also provided.
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Expanding the eukaryotic genetic code

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This invention provides compositions and methods for producing translational components that expand the number of genetically encoded amino acids in eukaryotic cells. The components include orthogonal tRNAs, orthogonal aminoacyl-tRNA synthetases, orthogonal pairs of tRNAs/synthetases and unnatural amino acids. Proteins and methods of producing proteins with unnatural amino acids in eukaryotic cells are also provided.
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Similar Records

Title: Expanding the eukaryotic genetic code

Abstract

Citation Formats

Engineering Chemical Reactivity on Cell Surfaces Through Oligosaccharide Biosynthesis journal, May 1997

Site-Specific Protein Modification Using a Ketone Handle journal, January 1996

Incorporation of azides into recombinant proteins for chemoselective modification by the Staudinger ligation journal, December 2001

Bioconjugation by Copper(I)-Catalyzed Azide-Alkyne [3 + 2] Cycloaddition journal, March 2003

An engineered Escherichia coli tyrosyl-tRNA synthetase for site-specific incorporation of an unnatural amino acid into proteins in eukaryotic translation and its application in a wheat germ cell-free system journal, July 2002

Addition of p-Azido-L-phenylalanine to the Genetic Code of Escherichia coli journal, July 2002

An Expanded Eukaryotic Genetic Code journal, August 2003

A New Strategy for the Site-Specific Modification of Proteins in Vivo journal, June 2003

Site-specific incorporation of biotinylated amino acids to identify surface-exposed residues in integral membrane proteins journal, October 1997

Misacylation of Yeast Amber Suppressor tRNATyr by E. coli Lysyl-tRNA Synthetase and Its Effective Repression by Genetic Engineering of the tRNA Sequence journal, April 2006

Chemoselective ligation reactions with proteins, oligosaccharides and cells journal, December 1998

Intermolecular 1,3-Dipolar Cycloadditions book, January 1991

Aliphatic amino azides as key building blocks for efficient polyamine syntheses journal, July 1993

Partition of tRNA synthetases into two classes based on mutually exclusive sets of sequence motifs journal, September 1990

Adding l-3-(2-Naphthyl)alanine to the Genetic Code of E. coli journal, March 2002

Recent advances in technology for measuring and manipulating cell signals journal, June 2000

Expanding tRNA recognition of a tRNA synthetase by a single amino acid change journal, May 2003

Addition of the keto functional group to the genetic code of Escherichia coli journal, December 2002

Unnatural amino acids as probes of protein structure and function journal, December 2000

Progress toward the evolution of an organism with an expanded genetic code journal, April 1999

Decomposition of Protein Tryptophan Fluorescence Spectra into Log-Normal Components. II. The Statistical Proof of Discreteness of Tryptophan Classes in Proteins journal, September 2001

Specific Covalent Labeling of Recombinant Protein Molecules Inside Live Cells journal, July 1998

Exploring the Limits of Codon and Anticodon Size journal, February 2002

Protein Tyrosine Kinase Csk-Catalyzed Phosphorylation of Src Containing Unnatural Tyrosine Analogues journal, September 2001

Regulated phosphorylation and dephosphorylation of GAL4, a transcriptional activator. journal, August 1989

Activity-Based Protein Profiling in Vivo Using a Copper(I)-Catalyzed Azide-Alkyne [3 + 2] Cycloaddition journal, April 2003

Peptidotriazoles on Solid Phase [1,2,3]-Triazoles by Regiospecific Copper(I)-Catalyzed 1,3-Dipolar Cycloadditions of Terminal Alkynes to Azides journal, May 2002

Addition of a photocrosslinking amino acid to the genetic code of Escherichia coli journal, August 2002

Expanding the genetic code: selection of efficient suppressors of four-base codons and identification of “shifty” four-base codons with a library approach in Escherichia coli journal, March 2001

Aminoacyl-tRNA synthetases: Versatile players in the changing theater of translation journal, November 2002

Imaging biochemistry inside cells journal, May 2001

Preparation of biological labels with acetylenic linker arms journal, January 1997

A Mutant Escherichia coli Tyrosyl-tRNA Synthetase Utilizes the Unnatural Amino Acid Azatyrosine More Efficiently than Tyrosine journal, September 2000

Adding Amino Acids with Novel Reactivity to the Genetic Code of Saccharomyces Cerevisiae journal, October 2003

Expanding the genetic code journal, December 2001

An expanding genetic code journal, December 2004

Progress Toward an Expanded Eukaryotic Genetic Code journal, June 2003

Cellular screening assays using fluorescence microscopy journal, October 2001

Programming peptidomimetic syntheses by translating genetic codes designed de novo journal, May 2003

A bacterial amber suppressor in Saccharomyces cerevisiae is selectively recognized by a bacterial aminoacyl-tRNA synthetase. journal, April 1990

Expanding the Genetic Code of Escherichia coli journal, April 2001

Synthesis and Photophysical Properties of Fluorescent Derivatives of Methylmercury journal, January 1996

Site-specific incorporation of an unnatural amino acid into proteins in mammalian cells journal, November 2002

Engineering Chemical Reactivity on Cell Surfaces Through Oligosaccharide Biosynthesis
journal, May 1997

Site-Specific Protein Modification Using a Ketone Handle
journal, January 1996

Incorporation of azides into recombinant proteins for chemoselective modification by the Staudinger ligation
journal, December 2001

Bioconjugation by Copper(I)-Catalyzed Azide-Alkyne [3 + 2] Cycloaddition
journal, March 2003

An engineered Escherichia coli tyrosyl-tRNA synthetase for site-specific incorporation of an unnatural amino acid into proteins in eukaryotic translation and its application in a wheat germ cell-free system
journal, July 2002

Addition of p-Azido-L-phenylalanine to the Genetic Code of Escherichia coli
journal, July 2002

An Expanded Eukaryotic Genetic Code
journal, August 2003

A New Strategy for the Site-Specific Modification of Proteins in Vivo
journal, June 2003

Site-specific incorporation of biotinylated amino acids to identify surface-exposed residues in integral membrane proteins
journal, October 1997

Misacylation of Yeast Amber Suppressor tRNATyr by E. coli Lysyl-tRNA Synthetase and Its Effective Repression by Genetic Engineering of the tRNA Sequence
journal, April 2006

Chemoselective ligation reactions with proteins, oligosaccharides and cells
journal, December 1998

Intermolecular 1,3-Dipolar Cycloadditions
book, January 1991

Aliphatic amino azides as key building blocks for efficient polyamine syntheses
journal, July 1993

Partition of tRNA synthetases into two classes based on mutually exclusive sets of sequence motifs
journal, September 1990

Adding l-3-(2-Naphthyl)alanine to the Genetic Code of E. coli
journal, March 2002

Recent advances in technology for measuring and manipulating cell signals
journal, June 2000

Expanding tRNA recognition of a tRNA synthetase by a single amino acid change
journal, May 2003

Addition of the keto functional group to the genetic code of Escherichia coli
journal, December 2002

Unnatural amino acids as probes of protein structure and function
journal, December 2000

Progress toward the evolution of an organism with an expanded genetic code
journal, April 1999

Decomposition of Protein Tryptophan Fluorescence Spectra into Log-Normal Components. II. The Statistical Proof of Discreteness of Tryptophan Classes in Proteins
journal, September 2001

Specific Covalent Labeling of Recombinant Protein Molecules Inside Live Cells
journal, July 1998

Exploring the Limits of Codon and Anticodon Size
journal, February 2002

Protein Tyrosine Kinase Csk-Catalyzed Phosphorylation of Src Containing Unnatural Tyrosine Analogues
journal, September 2001

Regulated phosphorylation and dephosphorylation of GAL4, a transcriptional activator.
journal, August 1989

Activity-Based Protein Profiling in Vivo Using a Copper(I)-Catalyzed Azide-Alkyne [3 + 2] Cycloaddition
journal, April 2003

Peptidotriazoles on Solid Phase [1,2,3]-Triazoles by Regiospecific Copper(I)-Catalyzed 1,3-Dipolar Cycloadditions of Terminal Alkynes to Azides
journal, May 2002

Addition of a photocrosslinking amino acid to the genetic code of Escherichia coli
journal, August 2002

Expanding the genetic code: selection of efficient suppressors of four-base codons and identification of “shifty” four-base codons with a library approach in Escherichia coli
journal, March 2001

Aminoacyl-tRNA synthetases: Versatile players in the changing theater of translation
journal, November 2002

Imaging biochemistry inside cells
journal, May 2001

Preparation of biological labels with acetylenic linker arms
journal, January 1997

A Mutant Escherichia coli Tyrosyl-tRNA Synthetase Utilizes the Unnatural Amino Acid Azatyrosine More Efficiently than Tyrosine
journal, September 2000

Adding Amino Acids with Novel Reactivity to the Genetic Code of Saccharomyces Cerevisiae
journal, October 2003

Expanding the genetic code
journal, December 2001

An expanding genetic code
journal, December 2004

Progress Toward an Expanded Eukaryotic Genetic Code
journal, June 2003

Cellular screening assays using fluorescence microscopy
journal, October 2001

Programming peptidomimetic syntheses by translating genetic codes designed de novo
journal, May 2003

A bacterial amber suppressor in Saccharomyces cerevisiae is selectively recognized by a bacterial aminoacyl-tRNA synthetase.
journal, April 1990

Expanding the Genetic Code of Escherichia coli
journal, April 2001

Synthesis and Photophysical Properties of Fluorescent Derivatives of Methylmercury
journal, January 1996

Site-specific incorporation of an unnatural amino acid into proteins in mammalian cells
journal, November 2002