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Title: Replica amplification of nucleic acid arrays

Abstract

Disclosed are improved methods of making and using immobilized arrays of nucleic acids, particularly methods for producing replicas of such arrays. Included are methods for producing high density arrays of nucleic acids and replicas of such arrays, as well as methods for preserving the resolution of arrays through rounds of replication. Also included are methods which take advantage of the availability of replicas of arrays for increased sensitivity in detection of sequences on arrays. Improved methods of sequencing nucleic acids immobilized on arrays utilizing single copies of arrays and methods taking further advantage of the availability of replicas of arrays are disclosed. The improvements lead to higher fidelity and longer read lengths of sequences immobilized on arrays. Methods are also disclosed which improve the efficiency of multiplex PCR using arrays of immobilized nucleic acids.

Inventors:
 [1];  [2]
  1. (Brookline, MA)
  2. (Chestnut Hill, MA)
Issue Date:
Research Org.:
President and Fellows of Harvard College (Cambridge, MA)
Sponsoring Org.:
USDOE
OSTI Identifier:
1013607
Patent Number(s):
7,785,790
Application Number:
11/745,806
Assignee:
President and Fellows of Harvard College (Cambridge, MA) CHO
DOE Contract Number:  
FG02-87ER60565
Resource Type:
Patent
Country of Publication:
United States
Language:
English

Citation Formats

Church, George M., and Mitra, Robi D. Replica amplification of nucleic acid arrays. United States: N. p., 2010. Web.
Church, George M., & Mitra, Robi D. Replica amplification of nucleic acid arrays. United States.
Church, George M., and Mitra, Robi D. Tue . "Replica amplification of nucleic acid arrays". United States. https://www.osti.gov/servlets/purl/1013607.
@article{osti_1013607,
title = {Replica amplification of nucleic acid arrays},
author = {Church, George M. and Mitra, Robi D.},
abstractNote = {Disclosed are improved methods of making and using immobilized arrays of nucleic acids, particularly methods for producing replicas of such arrays. Included are methods for producing high density arrays of nucleic acids and replicas of such arrays, as well as methods for preserving the resolution of arrays through rounds of replication. Also included are methods which take advantage of the availability of replicas of arrays for increased sensitivity in detection of sequences on arrays. Improved methods of sequencing nucleic acids immobilized on arrays utilizing single copies of arrays and methods taking further advantage of the availability of replicas of arrays are disclosed. The improvements lead to higher fidelity and longer read lengths of sequences immobilized on arrays. Methods are also disclosed which improve the efficiency of multiplex PCR using arrays of immobilized nucleic acids.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2010},
month = {8}
}

Patent:

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