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Title: Replica amplification of nucleic acid arrays

Abstract

Disclosed are improved methods of making and using immobilized arrays of nucleic acids, particularly methods for producing replicas of such arrays. Included are methods for producing high density arrays of nucleic acids and replicas of such arrays, as well as methods for preserving the resolution of arrays through rounds of replication. Also included are methods which take advantage of the availability of replicas of arrays for increased sensitivity in detection of sequences on arrays. Improved methods of sequencing nucleic acids immobilized on arrays utilizing single copies of arrays and methods taking further advantage of the availability of replicas of arrays are disclosed. The improvements lead to higher fidelity and longer read lengths of sequences immobilized on arrays. Methods are also disclosed which improve the efficiency of multiplex PCR using arrays of immobilized nucleic acids.

Inventors:
 [1];  [2]
  1. Brookline, MA
  2. Chestnut Hill, MA
Issue Date:
Research Org.:
President and Fellows of Harvard College (Cambridge, MA)
Sponsoring Org.:
USDOE
OSTI Identifier:
1013607
Patent Number(s):
7785790
Application Number:
11/745,806
Assignee:
President and Fellows of Harvard College (Cambridge, MA)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01J - CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY
DOE Contract Number:  
FG02-87ER60565
Resource Type:
Patent
Country of Publication:
United States
Language:
English

Citation Formats

Church, George M, and Mitra, Robi D. Replica amplification of nucleic acid arrays. United States: N. p., 2010. Web.
Church, George M, & Mitra, Robi D. Replica amplification of nucleic acid arrays. United States.
Church, George M, and Mitra, Robi D. Tue . "Replica amplification of nucleic acid arrays". United States. https://www.osti.gov/servlets/purl/1013607.
@article{osti_1013607,
title = {Replica amplification of nucleic acid arrays},
author = {Church, George M and Mitra, Robi D},
abstractNote = {Disclosed are improved methods of making and using immobilized arrays of nucleic acids, particularly methods for producing replicas of such arrays. Included are methods for producing high density arrays of nucleic acids and replicas of such arrays, as well as methods for preserving the resolution of arrays through rounds of replication. Also included are methods which take advantage of the availability of replicas of arrays for increased sensitivity in detection of sequences on arrays. Improved methods of sequencing nucleic acids immobilized on arrays utilizing single copies of arrays and methods taking further advantage of the availability of replicas of arrays are disclosed. The improvements lead to higher fidelity and longer read lengths of sequences immobilized on arrays. Methods are also disclosed which improve the efficiency of multiplex PCR using arrays of immobilized nucleic acids.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2010},
month = {8}
}

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Works referenced in this record:

Direct solid phase sequencing of genomic and plasmid DNA using magnetic beads as solid support
journal, January 1989


Ribosome display: an in vitro method for selection and evolution of antibodies from libraries
journal, December 1999


On the limiting pore size of hydrophilic gels for electrophoresis and isoelectric focussing
journal, June 1981


DNA polymerase fluorescent substrates with reversible 3′-tags
journal, October 1994


Synthesis of deoxyoligonucleotides on a polymer support
journal, June 1981


The application of DNA microarrays in gene expression analysis
journal, March 2000


Expression profiling using cDNA microarrays
journal, January 1999


Use of statistical criteria for screening potential homologies in nucleic acid sequences
journal, January 1984


Colony hybridization method for screening in situ of eukaryotic amplified genes
journal, May 1984


RAPD typing in microbiology—a technical review
journal, December 1996


Quantitative Monitoring of Gene Expression Patterns with a Complementary DNA Microarray
journal, October 1995


Preparation of Oligonucleotide-Biotin Conjugates with Cleavable Linkers
journal, January 1995


Exploring the Metabolic and Genetic Control of Gene Expression on a Genomic Scale
journal, October 1997


‘Laterally aggregated’ polyacrylamide gels for electrophoresis
journal, January 1992


Synthesis and Characterization of Biotin-Labeled Nucleotide Analogs
journal, June 1986


A chemically cleavable biotinylated nucleotide: usefulness in the recovery of protein-DNA complexes from avidin affinity columns.
journal, May 1985


Deoxynucleoside phosphoramidites—A new class of key intermediates for deoxypolynucleotide synthesis
journal, January 1981


RNA-peptide fusions for the in vitro selection of peptides and proteins
journal, November 1997


Nucleic acid fingerprinting by PCR-based methods: applications to problems in aging and mutagenesis
journal, October 1995