DOE Patents title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Polymerase chain reaction system using magnetic beads for analyzing a sample that includes nucleic acid

Abstract

A polymerase chain reaction system for analyzing a sample containing nucleic acid includes providing magnetic beads; providing a flow channel having a polymerase chain reaction chamber, a pre polymerase chain reaction magnet position adjacent the polymerase chain reaction chamber, and a post pre polymerase magnet position adjacent the polymerase chain reaction chamber. The nucleic acid is bound to the magnetic beads. The magnetic beads with the nucleic acid flow to the pre polymerase chain reaction magnet position in the flow channel. The magnetic beads and the nucleic acid are washed with ethanol. The nucleic acid in the polymerase chain reaction chamber is amplified. The magnetic beads and the nucleic acid are separated into a waste stream containing the magnetic beads and a post polymerase chain reaction mix containing the nucleic acid. The reaction mix containing the nucleic acid flows to an analysis unit in the channel for analysis.

Inventors:
 [1]
  1. Livermore, CA
Issue Date:
Research Org.:
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1013411
Patent Number(s):
7867713
Application Number:
12/106,390
Assignee:
LLNL
Patent Classifications (CPCs):
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01L - CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
DOE Contract Number:  
AC52-07NA27344
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Nasarabadi, Shanavaz. Polymerase chain reaction system using magnetic beads for analyzing a sample that includes nucleic acid. United States: N. p., 2011. Web.
Nasarabadi, Shanavaz. Polymerase chain reaction system using magnetic beads for analyzing a sample that includes nucleic acid. United States.
Nasarabadi, Shanavaz. Tue . "Polymerase chain reaction system using magnetic beads for analyzing a sample that includes nucleic acid". United States. https://www.osti.gov/servlets/purl/1013411.
@article{osti_1013411,
title = {Polymerase chain reaction system using magnetic beads for analyzing a sample that includes nucleic acid},
author = {Nasarabadi, Shanavaz},
abstractNote = {A polymerase chain reaction system for analyzing a sample containing nucleic acid includes providing magnetic beads; providing a flow channel having a polymerase chain reaction chamber, a pre polymerase chain reaction magnet position adjacent the polymerase chain reaction chamber, and a post pre polymerase magnet position adjacent the polymerase chain reaction chamber. The nucleic acid is bound to the magnetic beads. The magnetic beads with the nucleic acid flow to the pre polymerase chain reaction magnet position in the flow channel. The magnetic beads and the nucleic acid are washed with ethanol. The nucleic acid in the polymerase chain reaction chamber is amplified. The magnetic beads and the nucleic acid are separated into a waste stream containing the magnetic beads and a post polymerase chain reaction mix containing the nucleic acid. The reaction mix containing the nucleic acid flows to an analysis unit in the channel for analysis.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2011},
month = {1}
}

Works referenced in this record:

PCR amplification from single DNA molecules on magnetic beads in emulsion: application for high-throughput screening of transcription factor targets
journal, September 2005


Fabrication and characterization of polymer microfluidic devices for bio-agent detection
conference, January 2005


A multiplexed PCR-coupled liquid bead array for the simultaneous detection of four biothreat agents
journal, April 2005