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Title: Polynucleotides encoding anti-sulfotyrosine antibodies

Abstract

The invention provides anti-sulfotyrosine specific antibodies capable of detecting and isolating polypeptides that are tyrosine-sulfated. The sulfotyrosine antibodies and antibody fragments of the invention may be used to discriminate between the non-sulfated and sulfated forms of such proteins, using any number of immunological assays, such ELISAs, immunoblots, Western Blots, immunoprecipitations, and the like. Using a phage-display system, single chain antibodies (scFvs) were generated and screened against tyrosine-sulfated synthetic peptide antigens, resulting in the isolation of scFvs that specifically recognize sulfotyrosine-containing peptides and/or demonstrate sulfotyrosine-specific binding in tyrosine sulfated proteins. The VH and VL genes from one such sulfotyrosine-specific scFv were employed to generate a full length, sulfotyrosine-specific immunoglobulin.

Inventors:
 [1];  [2];  [3]
  1. Berkeley, CA
  2. Saint Davids, PA
  3. Santa Fe, NM
Issue Date:
Research Org.:
Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1013407
Patent Number(s):
7,868,152
Application Number:
12/560,294
Assignee:
Los Alamos National Security, LLC (Los Alamos, NM) ALO
DOE Contract Number:  
W-7405-ENG-36
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Bertozzi, Carolyn R, Kehoe, John, and Bradbury, Andrew M. Polynucleotides encoding anti-sulfotyrosine antibodies. United States: N. p., 2011. Web.
Bertozzi, Carolyn R, Kehoe, John, & Bradbury, Andrew M. Polynucleotides encoding anti-sulfotyrosine antibodies. United States.
Bertozzi, Carolyn R, Kehoe, John, and Bradbury, Andrew M. Tue . "Polynucleotides encoding anti-sulfotyrosine antibodies". United States. https://www.osti.gov/servlets/purl/1013407.
@article{osti_1013407,
title = {Polynucleotides encoding anti-sulfotyrosine antibodies},
author = {Bertozzi, Carolyn R and Kehoe, John and Bradbury, Andrew M},
abstractNote = {The invention provides anti-sulfotyrosine specific antibodies capable of detecting and isolating polypeptides that are tyrosine-sulfated. The sulfotyrosine antibodies and antibody fragments of the invention may be used to discriminate between the non-sulfated and sulfated forms of such proteins, using any number of immunological assays, such ELISAs, immunoblots, Western Blots, immunoprecipitations, and the like. Using a phage-display system, single chain antibodies (scFvs) were generated and screened against tyrosine-sulfated synthetic peptide antigens, resulting in the isolation of scFvs that specifically recognize sulfotyrosine-containing peptides and/or demonstrate sulfotyrosine-specific binding in tyrosine sulfated proteins. The VH and VL genes from one such sulfotyrosine-specific scFv were employed to generate a full length, sulfotyrosine-specific immunoglobulin.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2011},
month = {1}
}

Patent:

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