Lignin blockers and uses thereof
Abstract
Disclosed is a method for converting cellulose in a lignocellulosic biomass. The method provides for a lignin-blocking polypeptide and/or protein treatment of high lignin solids. The treatment enhances cellulase availability in cellulose conversion and allows for the determination of optimized pretreatment conditions. Additionally, ethanol yields from a Simultaneous Saccharification and Fermentation process are improved 5-25% by treatment with a lignin-blocking polypeptide and/or protein. Thus, a more efficient and economical method of processing lignin containing biomass materials utilizes a polypeptide/protein treatment step that effectively blocks lignin binding of cellulase.
- Inventors:
-
- West Lebanon, NH
- Norwich, VT
- Issue Date:
- Research Org.:
- Dartmouth College, Hanover, NH (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1013017
- Patent Number(s):
- 7875444
- Application Number:
- 11/229,817
- Assignee:
- The Trustees of Dartmouth College (Hanover, NH)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y02 - TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE Y02E - REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- DOE Contract Number:
- FC36-00GO10589; FC36-01GO11075
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Yang, Bin, and Wyman, Charles E. Lignin blockers and uses thereof. United States: N. p., 2011.
Web.
Yang, Bin, & Wyman, Charles E. Lignin blockers and uses thereof. United States.
Yang, Bin, and Wyman, Charles E. Tue .
"Lignin blockers and uses thereof". United States. https://www.osti.gov/servlets/purl/1013017.
@article{osti_1013017,
title = {Lignin blockers and uses thereof},
author = {Yang, Bin and Wyman, Charles E},
abstractNote = {Disclosed is a method for converting cellulose in a lignocellulosic biomass. The method provides for a lignin-blocking polypeptide and/or protein treatment of high lignin solids. The treatment enhances cellulase availability in cellulose conversion and allows for the determination of optimized pretreatment conditions. Additionally, ethanol yields from a Simultaneous Saccharification and Fermentation process are improved 5-25% by treatment with a lignin-blocking polypeptide and/or protein. Thus, a more efficient and economical method of processing lignin containing biomass materials utilizes a polypeptide/protein treatment step that effectively blocks lignin binding of cellulase.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2011},
month = {1}
}
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Works referenced in this record:
Evaluation of the cellobiose-fermenting yeastBrettanomyces custersii in the simultaneous saccharification and fermentation of cellulose
journal, May 1992
- Spindler, Diane D.; Wyman, Charles E.; Grohmann, Karel
- Biotechnology Letters, Vol. 14, Issue 5
Fast and efficient alkaline peroxide treatment to enhance the enzymatic digestibility of steam-exploded softwood substrates
journal, January 2002
- Yang, Bin; Boussaid, Abdel; Mansfield, Shawn D.
- Biotechnology and Bioengineering, Vol. 77, Issue 6
Investigation of the Cell-Wall Loosening Protein Expansin as a Possible Additive in the Enzymatic Saccharification of Lignocellulosic Biomass
journal, January 2000
- Baker, John O.; King, Michele R.; Adney, William S.
- Applied Biochemistry and Biotechnology, Vol. 84-86, Issue 1-9
Dilute-acid pretreatment of two short-rotation herbaceous crops: Scientific Note
journal, March 1992
- Torget, R.; Himmel, M.; Grohmann, K.
- Applied Biochemistry and Biotechnology, Vol. 34-35, Issue 1
Evaluation of Candida acidothermophilum in ethanol production from lignocellulosic biomass
journal, December 1997
- Kadam, K. L.; Schmidt, S. L.
- Applied Microbiology and Biotechnology, Vol. 48, Issue 6
The Reactions of Lignins with High Temperature Hydrogen Peroxide Part 2. The Oxidation of Kraft Lignin
journal, May 1999
- Kadla, J. F.; Chang, H. -m.; Jameel, H.
- Holzforschung, Vol. 53, Issue 3
Adsorption of high-purity endo-1,4-β-glucanases from Trichoderma reesei on components of lignocellulosic materials: Cellulose, lignin, and xylan
journal, August 1988
- Chernoglazov, Vladimir M.; Ermolova, Olga V.; Klyosov, Anatole A.
- Enzyme and Microbial Technology, Vol. 10, Issue 8, p. 503-507
Thermotolerant yeast for simultaneous saccharification and fermentation of cellulose to ethanol
journal, April 1988
- Spindler, Diane D.; Wyman, Charles E.; Mohagheghi, Ali
- Applied Biochemistry and Biotechnology, Vol. 17, Issue 1-3, p. 279-293
Factors affecting lignin degradation in lignocellulose by Phanerochaete chrysosporium
journal, February 1984
- Leisola, Matti S. A.; Ulmer, Duane C.; Fiechter, Armin
- Archives of Microbiology, Vol. 137, Issue 2
Lignin Impact on Fiber Degradation. 3. Reversal of Inhibition of Enzymatic Hydrolysis by Chemical Modification of Lignin and by Additives
journal, May 1997
- Sewalt, V. J. H.; Glasser, W. G.; Beauchemin, K. A.
- Journal of Agricultural and Food Chemistry, Vol. 45, Issue 5
Fermentability of the hemicellulose-derived sugars from steam-exploded softwood (douglas fir)
journal, August 1999
- Boussaid, Abdel; Robinson, Jamie; Cai, Yi-jin
- Biotechnology and Bioengineering, Vol. 64, Issue 3, p. 284-289
Biomass Ethanol: Technical Progress, Opportunities, and Commercial Challenges
journal, November 1999
- Wyman, Charles E.
- Annual Review of Energy and the Environment, Vol. 24, Issue 1, p. 189-226
Ethanol from lignocellulosic biomass: Technology, economics, and opportunities
journal, January 1994
- Wyman, Charles E.
- Bioresource Technology, Vol. 50, Issue 1, p. 3-15
Fractionation of Lignocellulosics by Steam-Aqueous Pretreatments [and Discussion]
journal, April 1987
- Overend, R. P.; Chornet, E.; Gascoigne, J. A.
- Philosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences, Vol. 321, Issue 1561
Simultaneous saccharification and fermentation of several lignocellulosic feedstocks to fuel ethanol
journal, January 1992
- Wyman, Charles E.; Spindler, Diane D.; Grohmann, Karel
- Biomass and Bioenergy, Vol. 3, Issue 5
The simultaneous saccharification and fermentation of pretreated woody crops to ethanol
journal, March 1991
- Spindler, Diane D.; Wyman, Charles E.; Grohmann, Karel
- Applied Biochemistry and Biotechnology, Vol. 28-29, Issue 1
Optimization of Steam Explosion to Enhance Hemicellulose Recovery and Enzymatic Hydrolysis of Cellulose in Softwoods
journal, January 1999
- Wu, Michael M.; Chang, Kevin; Gregg, David J.
- Applied Biochemistry and Biotechnology, Vol. 77, Issue 1-3
Twenty Years of Trials, Tribulations, and Research Progress in Bioethanol Technology: Selected Key Events Along the Way
journal, January 2001
- Wyman, Charles E.
- Applied Biochemistry and Biotechnology, Vol. 91-93, Issue 1-9
Fuel Ethanol Production from Lignocellulose Using Genetically Engineered Bacteria
book, May 1997
- Ingram, L. O.; Lai, X.; Moniruzzaman, M.
- Fuels and Chemicals from Biomass
Evaluation of pretreated herbaceous crops for the simultaneous saccharification and fermentation process
journal, March 1990
- Spindler, Diana; Wyman, Charles; Grohmann, Karel
- Applied Biochemistry and Biotechnology, Vol. 24-25, Issue 1
Cellulase Adsorption and an Evaluation of Enzyme Recycle During Hydrolysis of Steam-Exploded Softwood Residues
journal, January 2002
- Lu, Yanpin; Yang, Bin; Gregg, David
- Applied Biochemistry and Biotechnology, Vol. 98-100, Issue 1-9
Alkaline peroxide delignification of agricultural residues to enhance enzymatic saccharification
journal, January 1984
- Gould, J. Michael
- Biotechnology and Bioengineering, Vol. 26, Issue 1
Mechanism of surfactant effect in enzymatic hydrolysis of lignocellulose
journal, August 2002
- Eriksson, Torny; Börjesson, Johan; Tjerneld, Folke
- Enzyme and Microbial Technology, Vol. 31, Issue 3
Simultaneous saccharification and fermentation of pretreated wheat straw to ethanol with selected yeast strains and β-glucosidase supplementation
journal, January 1989
- Spindler, Diane D.; Wyman, Charles E.; Grohmann, Karel
- Applied Biochemistry and Biotechnology, Vol. 20-21, Issue 1
High solids simultaneous saccharification and fermentation of pretreated wheat straw to ethanol
journal, May 1992
- Mohagheghi, A.; Tucker, M.; Grohmann, K.
- Applied Biochemistry and Biotechnology, Vol. 33, Issue 2
Simultaneous saccharification and fermentation (SSF) of lignocellulosics to ethanol under vacuum cycling and step feeding
journal, April 1984
- Ghose, T. K.; Roychoudhury, P. K.; Ghosh, P.
- Biotechnology and Bioengineering, Vol. 26, Issue 4