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Title: Method for analyzing microbial communities

Abstract

The present invention provides a method for quantitatively analyzing microbial genes, species, or strains in a sample that contains at least two species or strains of microorganisms. The method involves using an isothermal DNA polymerase to randomly and representatively amplify genomic DNA of the microorganisms in the sample, hybridizing the resultant polynucleotide amplification product to a polynucleotide microarray that can differentiate different genes, species, or strains of microorganisms of interest, and measuring hybridization signals on the microarray to quantify the genes, species, or strains of interest.

Inventors:
 [1];  [1]
  1. Oak Ridge, TN
Issue Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1012821
Patent Number(s):
7759057
Application Number:
10/943,067
Assignee:
UT-Battelle, LLC (Oak Ridge, TN)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
DOE Contract Number:  
AC05-00OR22725
Resource Type:
Patent
Country of Publication:
United States
Language:
English

Citation Formats

Zhou, Jizhong, and Wu, Liyou. Method for analyzing microbial communities. United States: N. p., 2010. Web.
Zhou, Jizhong, & Wu, Liyou. Method for analyzing microbial communities. United States.
Zhou, Jizhong, and Wu, Liyou. Tue . "Method for analyzing microbial communities". United States. https://www.osti.gov/servlets/purl/1012821.
@article{osti_1012821,
title = {Method for analyzing microbial communities},
author = {Zhou, Jizhong and Wu, Liyou},
abstractNote = {The present invention provides a method for quantitatively analyzing microbial genes, species, or strains in a sample that contains at least two species or strains of microorganisms. The method involves using an isothermal DNA polymerase to randomly and representatively amplify genomic DNA of the microorganisms in the sample, hybridizing the resultant polynucleotide amplification product to a polynucleotide microarray that can differentiate different genes, species, or strains of microorganisms of interest, and measuring hybridization signals on the microarray to quantify the genes, species, or strains of interest.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2010},
month = {7}
}

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Works referenced in this record:

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DNA/DNA hybridization to microarrays reveals gene-specific differences between closely related microbial genomes
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Isothermal Strand-Displacement Amplification Applications for High-Throughput Genomics
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