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Title: Enzyme system comprising an enzyme bonded in a porous matrix

Abstract

A protein system is described in which a protein is bound within a matrix material that has pores that are sized to achieve excellent properties such as: activity, protein density, and stability. In a preferred embodiment, the pore sizes range from 50 to 400 .ANG.. One protein that has demonstrated surprisingly good results in this system is OPH. This protein is known to degrade organophosphorus compounds such as are found in chemical weapons and pesticides. Novel methods of forming the protein system and methods of making OPH are also described.

Inventors:
 [1];  [2]
  1. Richland, WA
  2. West Richland, WA
Issue Date:
Research Org.:
Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1011441
Patent Number(s):
7846684
Application Number:
09/791,138
Assignee:
Battelle Memorial Institute (Richland, WA)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
DOE Contract Number:  
AC06-76RL01830
Resource Type:
Patent
Country of Publication:
United States
Language:
English

Citation Formats

Ackerman, Eric, and Liu, Jun. Enzyme system comprising an enzyme bonded in a porous matrix. United States: N. p., 2010. Web.
Ackerman, Eric, & Liu, Jun. Enzyme system comprising an enzyme bonded in a porous matrix. United States.
Ackerman, Eric, and Liu, Jun. Tue . "Enzyme system comprising an enzyme bonded in a porous matrix". United States. https://www.osti.gov/servlets/purl/1011441.
@article{osti_1011441,
title = {Enzyme system comprising an enzyme bonded in a porous matrix},
author = {Ackerman, Eric and Liu, Jun},
abstractNote = {A protein system is described in which a protein is bound within a matrix material that has pores that are sized to achieve excellent properties such as: activity, protein density, and stability. In a preferred embodiment, the pore sizes range from 50 to 400 .ANG.. One protein that has demonstrated surprisingly good results in this system is OPH. This protein is known to degrade organophosphorus compounds such as are found in chemical weapons and pesticides. Novel methods of forming the protein system and methods of making OPH are also described.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2010},
month = {12}
}

Works referenced in this record:

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Biosensor for direct determination of organophosphate nerve agents. 1. Potentiometric enzyme electrode
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Expression, immobilization, and enzymatic characterization of cellulose-binding domain-organophosphorus hydrolase fusion enzymes
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Reusable immobilized enzyme/polyurethane sponge for removal and detoxification of localized organophosphate pesticide spills
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Porous zirconia: a new support material for enzyme immobilization
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Technology and Applications of Microengineered Reactors
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Mesoporous molecular sieve immobilized enzymes
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Functionalized Monolayers on Ordered Mesoporous Supports
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