DOE Patents title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Study of polymer molecules and conformations with a nanopore

Abstract

The invention features methods for evaluating the conformation of a polymer, for example, for determining the conformational distribution of a plurality of polymers and to detect binding or denaturation events. The methods employ a nanopore which the polymer, e.g., a nucleic acid, traverses. As the polymer traverses the nanopore, measurements of transport properties of the nanopore yield data on the conformation of the polymer.

Inventors:
 [1];  [2];  [3];  [4]
  1. Lexington, MA
  2. Fayetteville, AR
  3. Delft, NL
  4. Cambridge, MA
Issue Date:
Research Org.:
Harvard College
Sponsoring Org.:
USDOE
OSTI Identifier:
1011416
Patent Number(s):
7846738
Application Number:
10/918,959
Assignee:
CHO
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y10 - TECHNICAL SUBJECTS COVERED BY FORMER USPC Y10T - TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
DOE Contract Number:  
US patent application 10/918,959
Resource Type:
Patent
Country of Publication:
United States
Language:
English

Citation Formats

Golovchenko, Jene A, Li, Jiali, Stein, Derek, and Gershow, Marc H. Study of polymer molecules and conformations with a nanopore. United States: N. p., 2010. Web.
Golovchenko, Jene A, Li, Jiali, Stein, Derek, & Gershow, Marc H. Study of polymer molecules and conformations with a nanopore. United States.
Golovchenko, Jene A, Li, Jiali, Stein, Derek, and Gershow, Marc H. Tue . "Study of polymer molecules and conformations with a nanopore". United States. https://www.osti.gov/servlets/purl/1011416.
@article{osti_1011416,
title = {Study of polymer molecules and conformations with a nanopore},
author = {Golovchenko, Jene A and Li, Jiali and Stein, Derek and Gershow, Marc H},
abstractNote = {The invention features methods for evaluating the conformation of a polymer, for example, for determining the conformational distribution of a plurality of polymers and to detect binding or denaturation events. The methods employ a nanopore which the polymer, e.g., a nucleic acid, traverses. As the polymer traverses the nanopore, measurements of transport properties of the nanopore yield data on the conformation of the polymer.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2010},
month = {12}
}

Works referenced in this record:

Ion-beam sculpting at nanometre length scales
journal, July 2001


Alignment and sensitive detection of DNA by a moving interface
journal, September 1994


Detecting protein analytes that modulate transmembrane movement of a polymer chain within a single protein pore
journal, October 2000


Transcription by T7 RNA polymerase using benzo[a]pyrene-modified templates
journal, January 1991


DNA molecules and configurations in a solid-state nanopore microscope
journal, August 2003


Permissive sites and topology of an outer membrane protein with a reporter epitope.
journal, January 1991


Pore formation by LamB of Escherichia coli in lipid bilayer membranes.
journal, January 1986


RNA polymerase-dependent mechanism for the stepwise T7 phage DNA transport from the virion into E. coli
journal, January 1982


Voltage-Driven DNA Translocations through a Nanopore
journal, April 2001


"Reversed" alamethicin conductance in lipid bilayers
journal, April 1991


Fluorescence measurement of the kinetics of DNA injection by bacteriophage .lambda. into liposomes
journal, October 1988


Single-channel currents recorded from membrane of denervated frog muscle fibres
journal, April 1976


The voltage-dependent activity of Escherichia coli porins in different planar bilayer reconstitutions
journal, December 1989


Images of a lipid bilayer at molecular resolution by scanning tunneling microscopy.
journal, February 1987


Nanopores with a spark for single-molecule detection
journal, July 2001


Electrokinetic measurements with submicron particles and pores by the resistive pulse technique
journal, September 1977


Induction of DNA replication by transcription in the region upstream of the human c-myc gene in a model replication system.
journal, October 1996


Characterization of Nucleic Acids by Nanopore Analysis
journal, October 2002


Single-molecule analysis of DNA-protein complexes using nanopores
journal, March 2007


Stochastic sensors inspired by biology
journal, September 2001


Rapid discrimination among individual DNA hairpin molecules at single-nucleotide resolution using an ion channel
journal, March 2001


Adapting to nanoscale events
journal, April 1999


Structure of large fragment of Escherichia coli DNA polymerase I complexed with dTMP
journal, February 1985


Artificial transmembrane ion channels from self-assembling peptide nanotubes
journal, May 1994


DNA conformation and base number simultaneously determined in a nanopore
journal, September 2007


Genetic analysis of membrane protein topology by a sandwich gene fusion approach.
journal, October 1990


Electroporation and electrophoretic DNA transfer into cells. The effect of DNA interaction with electropores
journal, November 1992


Open channel noise. V. Fluctuating barriers to ion entry in gramicidin A channels
journal, March 1990


Mechanism of sugar transport through the sugar-specific LamB channel ofEscherichia coli outer membrane
journal, December 1987


Optimizing planar lipid bilayer single-channel recordings for high resolution with rapid voltage steps
journal, August 1990


Mutagenesis by random linker insertion into the lamB gene of Escherichia coli K12
journal, November 1986


Rapid nanopore discrimination between single polynucleotide molecules
journal, February 2000


Domain of E. coli DNA polymerase I showing sequence homology to T7 DNA polymerase
journal, February 1985


A protein-conducting channel in the endoplasmic reticulum
journal, May 1991


Sequencing and the Single Channel
journal, December 1999


Amino acid residues required for fast Na(+)-channel inactivation: charge neutralizations and deletions in the III-IV linker.
journal, November 1992


A neutral amino acid change in segment IIS4 dramatically alters the gating properties of the voltage-dependent sodium channel.
journal, January 1990


Characterization of individual polynucleotide molecules using a membrane channel
journal, November 1996


Determinants of membrane protein topology.
journal, December 1987


Sequence-specific detection of individual DNA strands using engineered nanopores
journal, July 2001


Hydrophobic substitution mutations in the S4 sequence alter voltage-dependent gating in shaker K+ channels
journal, August 1991


Open channel noise. IV. Estimation of rapid kinetics of formamide block in gramicidin A channels
journal, October 1988


The potential and challenges of nanopore sequencing
book, August 2009


Nanopores and nucleic acids: prospects for ultrarapid sequencing
journal, April 2000


Open channel noise. III. High-resolution recordings show rapid current fluctuations in gramicidin A and four chemical analogues
journal, December 1987


Unzipping Kinetics of Double-Stranded DNA in a Nanopore
journal, June 2003


Electronic Counting and Sizing of Bacteria
journal, July 1958


Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches
journal, August 1981


Effect of point mutations on the in-vitro pore properties of maltoporin, a protein of Escherichia coli outer membrane
journal, June 1988


Selectivity for maltose and maltodextrins of maltoporin, a pore-forming protein of E. coli outer membrane
journal, August 1987


Molecular architecture and electrostatic properties of a bacterial porin
journal, December 1991


Two types of inactivation in Shaker K+ channels: Effects of alterations in the carboxy-terminal region
journal, October 1991


SpoIIIE strips proteins off the DNA during chromosome translocation
journal, July 2008


Single Ion-Channel Recordings Using Glass Nanopore Membranes
journal, September 2007


Biophysical and molecular mechanisms of Shaker potassium channel inactivation
journal, October 1990


Sandwiched Graphene−Membrane Superstructures
journal, December 2009


A cluster of hydrophobic amino acid residues required for fast Na(+)-channel inactivation.
journal, November 1992


Improved stability of black lipid membranes by coating with polysaccharide derivatives bearing hydrophobic anchor groups
journal, May 1986


Alamethicin. A rich model for channel behavior
journal, January 1984


Blockade of a mitochondrial cationic channel by an addressing peptide: An electrophysiological study
journal, December 1989


Counting polymers moving through a single ion channel
journal, July 1994