Osur_paper

Kiefer, Julian

doi:10.25982/58799.728/1798340

Title: Osur_paper

Abstract

Glyphosate is widely used as a herbicide, but recent studies begin to reveal its detrimental side effects on animals by targeting the shikimate pathway of associated gut microorganisms. However, its impact on nutritional endosymbionts in insects remains poorly understood. Here, we sequenced the tiny, shikimate pathway encoding symbiont genome of the sawtoothed grain beetle Oryzaephilus surinamensis. Decreased titers of the aromatic amino acid tyrosine in symbiont-depleted beetles underscore the symbionts’ ability to synthesize prephenate as the precursor for host tyrosine synthesis and its importance for cuticle sclerotization and melanization. Glyphosate exposure inhibited symbiont establishment during host development and abolished the mutualistic benefit on cuticle synthesis in adults, which could be partially rescued by dietary tyrosine supplementation. Furthermore, phylogenetic analyses indicate that the shikimate pathways of many nutritional endosymbionts likewise contain a glyphosate sensitive 5-enolpyruvylshikimate-3-phosphate synthase. These findings highlight the importance of symbiont-mediated tyrosine supplementation for cuticle biosynthesis in insects, but also paint an alarming scenario regarding the use of glyphosate in light of recent declines in insect populations.

Authors:

Johannes Gutenberg Universität Mainz, Mainz, Rheinland-Pfalz, DE

Publication Date:: 2021-06-23

Research Org.:: Johannes Gutenberg Universität Mainz, Mainz, Rheinland-Pfalz, DE

Sponsoring Org.:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Subject:: 59 BASIC BIOLOGICAL SCIENCES

Keywords:: symbiont, glyphosate

OSTI Identifier:: 1798340

DOI:: https://doi.org/10.25982/58799.728/1798340

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                    Kiefer, Julian. Osur_paper.  United States: N. p., 2021. 
        Web.  doi:10.25982/58799.728/1798340.

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                    Kiefer, Julian. Osur_paper.  United States.  doi:https://doi.org/10.25982/58799.728/1798340

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                    Kiefer, Julian. 2021.  
"Osur_paper".  United States.  doi:https://doi.org/10.25982/58799.728/1798340.  https://www.osti.gov/servlets/purl/1798340. Pub date:Wed Jun 23 00:00:00 EDT 2021

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@article{osti_1798340,

  title        = {Osur_paper},

  author       = {Kiefer, Julian},

  abstractNote = {Glyphosate is widely used as a herbicide, but recent studies begin to reveal its detrimental side effects on animals by targeting the shikimate pathway of associated gut microorganisms. However, its impact on nutritional endosymbionts in insects remains poorly understood. Here, we sequenced the tiny, shikimate pathway encoding symbiont genome of the sawtoothed grain beetle Oryzaephilus surinamensis. Decreased titers of the aromatic amino acid tyrosine in symbiont-depleted beetles underscore the symbionts’ ability to synthesize prephenate as the precursor for host tyrosine synthesis and its importance for cuticle sclerotization and melanization. Glyphosate exposure inhibited symbiont establishment during host development and abolished the mutualistic benefit on cuticle synthesis in adults, which could be partially rescued by dietary tyrosine supplementation. Furthermore, phylogenetic analyses indicate that the shikimate pathways of many nutritional endosymbionts likewise contain a glyphosate sensitive 5-enolpyruvylshikimate-3-phosphate synthase. These findings highlight the importance of symbiont-mediated tyrosine supplementation for cuticle biosynthesis in insects, but also paint an alarming scenario regarding the use of glyphosate in light of recent declines in insect populations.},

  doi          = {10.25982/58799.728/1798340},

  journal      = {},

  number       = ,

  volume       = ,

  place        = {United States},

  year         = {2021},

  month        = {6}

}

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DOI: https://doi.org/10.25982/58799.728/1798340

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Similar Records

Title: Osur_paper

Abstract

Citation Formats

Prokka: rapid prokaryotic genome annotation journal, March 2014

TIGRFAMs and Genome Properties in 2013 journal, November 2012

Accelerated Profile HMM Searches journal, October 2011

Trimmomatic: a flexible trimmer for Illumina sequence data journal, April 2014

SPAdes: A New Genome Assembly Algorithm and Its Applications to Single-Cell Sequencing journal, May 2012

The Pfam protein families database: towards a more sustainable future journal, December 2015

KBase: The United States Department of Energy Systems Biology Knowledgebase journal, July 2018

A Genomic Perspective on Protein Families journal, October 1997

Accelerated Profile HMM Searches journal, October 2011

CheckM: assessing the quality of microbial genomes recovered from isolates, single cells, and metagenomes journal, May 2015

OrthoMCL: Identification of Ortholog Groups for Eukaryotic Genomes journal, September 2003

The RAST Server: Rapid Annotations using Subsystems Technology journal, January 2008

FIGfams: yet another set of protein families journal, September 2009

BLAT---The BLAST-Like Alignment Tool journal, March 2002

CDD/SPARCLE: functional classification of proteins via subfamily domain architectures journal, November 2016

Improved metagenomic analysis with Kraken 2 journal, November 2019

PhiSpy: a novel algorithm for finding prophages in bacterial genomes that combines similarity- and composition-based strategies journal, May 2012

BLAST+: architecture and applications journal, January 2009

Kraken: ultrafast metagenomic sequence classification using exact alignments journal, January 2014

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Inhibition of a nutritional endosymbiont by glyphosate abolishes mutualistic benefit on cuticle synthesis in Oryzaephilus surinamensis journal, May 2021

Prodigal: prokaryotic gene recognition and translation initiation site identification journal, March 2010

Identification, variation and transcription of pneumococcal repeat sequences journal, February 2011

Prodigal: prokaryotic gene recognition and translation initiation site identification journal, March 2010

Ancient symbiosis confers desiccation resistance to stored grain pest beetles journal, November 2017

TIGRFAMs and Genome Properties: tools for the assignment of molecular function and biological process in prokaryotic genomes journal, January 2007

20 years of the SMART protein domain annotation resource journal, October 2017

RASTtk: A modular and extensible implementation of the RAST algorithm for building custom annotation pipelines and annotating batches of genomes journal, February 2015

The SEED and the Rapid Annotation of microbial genomes using Subsystems Technology (RAST) journal, November 2013

BusyBee Web: metagenomic data analysis by bootstrapped supervised binning and annotation journal, May 2017

Identifying bacterial genes and endosymbiont DNA with Glimmer journal, January 2007

BLAST+: architecture and applications journal, January 2009

The RAST Server: Rapid Annotations using Subsystems Technology journal, January 2008

Identification, variation and transcription of pneumococcal repeat sequences journal, February 2011

FastTree 2 – Approximately Maximum-Likelihood Trees for Large Alignments journal, March 2010

Translational recoding in archaea journal, September 2012

Prokka: rapid prokaryotic genome annotation
journal, March 2014

TIGRFAMs and Genome Properties in 2013
journal, November 2012

Accelerated Profile HMM Searches
journal, October 2011

Trimmomatic: a flexible trimmer for Illumina sequence data
journal, April 2014

SPAdes: A New Genome Assembly Algorithm and Its Applications to Single-Cell Sequencing
journal, May 2012

The Pfam protein families database: towards a more sustainable future
journal, December 2015

KBase: The United States Department of Energy Systems Biology Knowledgebase
journal, July 2018

A Genomic Perspective on Protein Families
journal, October 1997

Accelerated Profile HMM Searches
journal, October 2011

CheckM: assessing the quality of microbial genomes recovered from isolates, single cells, and metagenomes
journal, May 2015

OrthoMCL: Identification of Ortholog Groups for Eukaryotic Genomes
journal, September 2003

The RAST Server: Rapid Annotations using Subsystems Technology
journal, January 2008

FIGfams: yet another set of protein families
journal, September 2009

BLAT---The BLAST-Like Alignment Tool
journal, March 2002

CDD/SPARCLE: functional classification of proteins via subfamily domain architectures
journal, November 2016

Improved metagenomic analysis with Kraken 2
journal, November 2019

PhiSpy: a novel algorithm for finding prophages in bacterial genomes that combines similarity- and composition-based strategies
journal, May 2012

BLAST+: architecture and applications
journal, January 2009

Kraken: ultrafast metagenomic sequence classification using exact alignments
journal, January 2014

Gapped BLAST and PSI-BLAST: a new generation of protein database search programs
journal, September 1997

Inhibition of a nutritional endosymbiont by glyphosate abolishes mutualistic benefit on cuticle synthesis in Oryzaephilus surinamensis
journal, May 2021

Prodigal: prokaryotic gene recognition and translation initiation site identification
journal, March 2010

Identification, variation and transcription of pneumococcal repeat sequences
journal, February 2011

Prodigal: prokaryotic gene recognition and translation initiation site identification
journal, March 2010

Ancient symbiosis confers desiccation resistance to stored grain pest beetles
journal, November 2017

TIGRFAMs and Genome Properties: tools for the assignment of molecular function and biological process in prokaryotic genomes
journal, January 2007

20 years of the SMART protein domain annotation resource
journal, October 2017

RASTtk: A modular and extensible implementation of the RAST algorithm for building custom annotation pipelines and annotating batches of genomes
journal, February 2015

The SEED and the Rapid Annotation of microbial genomes using Subsystems Technology (RAST)
journal, November 2013

BusyBee Web: metagenomic data analysis by bootstrapped supervised binning and annotation
journal, May 2017

Identifying bacterial genes and endosymbiont DNA with Glimmer
journal, January 2007

BLAST+: architecture and applications
journal, January 2009

The RAST Server: Rapid Annotations using Subsystems Technology
journal, January 2008

Identification, variation and transcription of pneumococcal repeat sequences
journal, February 2011

FastTree 2 – Approximately Maximum-Likelihood Trees for Large Alignments
journal, March 2010

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