S-Layer Nanosheet Binding of Zn and Gd
Abstract
This data characterizes binding of Zn2+ and Gd3+ to engineered nanosheets at 40C and in a brine solution. The engineered nanosheets are composed of surface-layer (S-layer) proteins which form 2 D crystalline sheets and display Zn2+- or Gd3+-binding domains on these sheets. Their ability to bind Zn2+ is compared to S-layer nanosheets that do not contain Zn2+-binding domains. We found that the purification method of these nanosheets was a critical determinant of their function and thus have provided data on the binding from two different purification methods. A key distinction of this dataset from other datasets is that the engineered nanosheets were expressed and purified from E. coli grown at 37C as described in (Kinns, 2010; Howorka, 2000), References: Kinns, H., et al. Identifying assembly-inhibiting and assembly-tolerant sites in the SbsB S-layer protein from Geobacillus stearothermophilus. Journal of Molecular Biology, 2010. 395(4): p. 742-753. Howorka, S., et al. Surface-accessible residues in the monomeric and assembled forms of a bacterial surface layer protein. Journal of Biological Chemistry, 2000. 275(48): p. 37876-37886.
- Publication Date:
- Other Number(s):
- 951
- DOE Contract Number:
- FY15 AOP 2514
- Product Type:
- Dataset
- Research Org.:
- DOE Geothermal Data Repository; Lawrence Berkeley National Laboratory
- Sponsoring Org.:
- USDOE Office of Energy Efficiency and Renewable Energy (EERE), Geothermal Technologies Program (EE-2C)
- Subject:
- 15 Geothermal Energy
- Keywords:
- geothermal; energy; mineral recovery; synthetic biology; S-layer; metal-binding domain; nanosheet; brine study; binding; chemistry; Gadolinium; zinc; gd3; zn2; ion
- OSTI Identifier:
- 1372392
- DOI:
- 10.15121/1372392
Citation Formats
Ajo-Franklin, Caroline, Charrier, Marimikel, and Yang, Li. S-Layer Nanosheet Binding of Zn and Gd. United States: N. p., 2016.
Web. doi:10.15121/1372392.
Ajo-Franklin, Caroline, Charrier, Marimikel, & Yang, Li. S-Layer Nanosheet Binding of Zn and Gd. United States. doi:10.15121/1372392.
Ajo-Franklin, Caroline, Charrier, Marimikel, and Yang, Li. 2016.
"S-Layer Nanosheet Binding of Zn and Gd". United States. doi:10.15121/1372392. https://www.osti.gov/servlets/purl/1372392. Pub date:Fri Apr 15 00:00:00 EDT 2016
@article{osti_1372392,
title = {S-Layer Nanosheet Binding of Zn and Gd},
author = {Ajo-Franklin, Caroline and Charrier, Marimikel and Yang, Li},
abstractNote = {This data characterizes binding of Zn2+ and Gd3+ to engineered nanosheets at 40C and in a brine solution. The engineered nanosheets are composed of surface-layer (S-layer) proteins which form 2 D crystalline sheets and display Zn2+- or Gd3+-binding domains on these sheets. Their ability to bind Zn2+ is compared to S-layer nanosheets that do not contain Zn2+-binding domains. We found that the purification method of these nanosheets was a critical determinant of their function and thus have provided data on the binding from two different purification methods. A key distinction of this dataset from other datasets is that the engineered nanosheets were expressed and purified from E. coli grown at 37C as described in (Kinns, 2010; Howorka, 2000), References: Kinns, H., et al. Identifying assembly-inhibiting and assembly-tolerant sites in the SbsB S-layer protein from Geobacillus stearothermophilus. Journal of Molecular Biology, 2010. 395(4): p. 742-753. Howorka, S., et al. Surface-accessible residues in the monomeric and assembled forms of a bacterial surface layer protein. Journal of Biological Chemistry, 2000. 275(48): p. 37876-37886.},
doi = {10.15121/1372392},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2016},
month = {4}
}