Title: Imaging single cells in a beam of live cyanobacteria with an X-ray laser (CXIDB ID 27)

Abstract

Diffraction pattern of a micron-sized S. elongatus cell at 1,100 eV photon energy (1.13 nm wavelength) with ~10^11 photons per square micron on the sample in ~70 fs. The signal to noise ratio at 4 nm resolution is 3.7 with 0.24 photons per Nyquist pixel. The cell was alive at the time of the exposure. The central region of the pattern (dark red) is saturated and this prevented reliable image reconstruction.

Publication Date:
Other Number(s):
CXIDB ID 27
DOE Contract Number:  
AC02-05CH11231;
Research Org.:
Coherent X-ray Imaging Data Bank (Lawrence Berkeley National Laboratory); Uppsala University
Sponsoring Org.:
Uppsala University
Subject:
AMO; LCLS; Single Particle X-ray Diffraction Imaging; Synechococcus elongatus; X-ray Free-electorn Lasers; XFEL
OSTI Identifier:
1169687
DOI:
https://doi.org/10.11577/1169687

Citation Formats

Imaging single cells in a beam of live cyanobacteria with an X-ray laser (CXIDB ID 27). United States: N. p., 2015. Web. doi:10.11577/1169687.
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Imaging single cells in a beam of live cyanobacteria with an X-ray laser (CXIDB ID 27). United States. doi:https://doi.org/10.11577/1169687
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2015. "Imaging single cells in a beam of live cyanobacteria with an X-ray laser (CXIDB ID 27)". United States. doi:https://doi.org/10.11577/1169687. https://www.osti.gov/servlets/purl/1169687. Pub date:Mon Feb 09 19:00:00 EST 2015
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@article{osti_1169687,
title = {Imaging single cells in a beam of live cyanobacteria with an X-ray laser (CXIDB ID 27)},
abstractNote = {Diffraction pattern of a micron-sized S. elongatus cell at 1,100 eV photon energy (1.13 nm wavelength) with ~10^11 photons per square micron on the sample in ~70 fs. The signal to noise ratio at 4 nm resolution is 3.7 with 0.24 photons per Nyquist pixel. The cell was alive at the time of the exposure. The central region of the pattern (dark red) is saturated and this prevented reliable image reconstruction.},
doi = {10.11577/1169687},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2015},
month = {2}
}
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DOI: https://doi.org/10.11577/1169687
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