Rare Earth Desorption Test with Citrate and Bicarbonate

Jiao, Yongqin; Brewer, Aaron; Park, Dan

doi:10.15121/1452718

Title: Rare Earth Desorption Test with Citrate and Bicarbonate

Dataset
Other Related Research

Abstract

LBT (lanthanide binding tag) cells were grown overnight in LB media with 0.05% Amp. 1:100 subculture taken from overnights, grown for 2 hours. LBT was induced with 0.002% arabinose added for 3 hours. REE adsorption was done by combining 350 ul (0.25% 1M MES, 12.5 uM Tb, and 12.5 uM La or Cu in sterile DI water) and 350 ul (LBT cells with OD = 1 in 10 mM MES), reacted for approx. 30 min. Following adsorption, citrate and bicarbonate solutions were used in desorption to recover rare earth from cell surface, and to further separate REE from non-REEs. The samples were then centrifuged and a fraction of the supernatant was collected for ICP-MS analysis.

Authors:: Jiao, Yongqin; Brewer, Aaron; Park, Dan

Publication Date:: Thu Jun 01 00:00:00 EDT 2017

Other Number(s):: 965

DOE Contract Number:: LLNL FY17 AOP 2.5.1.12

Research Org.:: USDOE Geothermal Data Repository (United States); Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)

Sponsoring Org.:: USDOE Office of Energy Efficiency and Renewable Energy (EERE), Geothermal Technologies Program (EE-2C)

Collaborations:: Lawrence Livermore National Laboratory

Subject:: 15 Geothermal Energy

Keywords:: geothermal; energy; adsorption; desorption; rare earth; citrate; bicarbonate; REE; resource recovery; centrifuge; brine; lbt; lanthanide binding tag

Geolocation:: 39.295415290912,-118.3872640625|35.15607354867,-118.3872640625|35.15607354867,-122.2433640625|39.295415290912,-122.2433640625|39.295415290912,-118.3872640625

OSTI Identifier:: 1452718

DOI:: https://doi.org/10.15121/1452718

Project Location:

Citation Formats


                    Jiao, Yongqin, Brewer, Aaron, and Park, Dan. Rare Earth Desorption Test with Citrate and Bicarbonate.  United States: N. p., 2017. 
        Web.  doi:10.15121/1452718.

Copy to clipboard


                    Jiao, Yongqin, Brewer, Aaron, & Park, Dan. Rare Earth Desorption Test with Citrate and Bicarbonate.  United States.  doi:https://doi.org/10.15121/1452718

Copy to clipboard


                    Jiao, Yongqin, Brewer, Aaron, and Park, Dan. 2017.  
"Rare Earth Desorption Test with Citrate and Bicarbonate".  United States.  doi:https://doi.org/10.15121/1452718.  https://www.osti.gov/servlets/purl/1452718. Pub date:Thu Jun 01 00:00:00 EDT 2017

Copy to clipboard


                    
@article{osti_1452718,

  title        = {Rare Earth Desorption Test with Citrate and Bicarbonate},

  author       = {Jiao, Yongqin and Brewer, Aaron and Park, Dan},

  abstractNote = {LBT (lanthanide binding tag) cells were grown overnight in LB media with 0.05% Amp. 1:100 subculture taken from overnights, grown for 2 hours. LBT was induced with 0.002% arabinose added for 3 hours. REE adsorption was done by combining 350 ul (0.25% 1M MES, 12.5 uM Tb, and 12.5 uM La or Cu in sterile DI water) and 350 ul (LBT cells with OD = 1 in 10 mM MES), reacted for approx. 30 min. Following adsorption, citrate and bicarbonate solutions were used in desorption to recover rare earth from cell surface, and to further separate REE from non-REEs. The samples were then centrifuged and a fraction of the supernatant was collected for ICP-MS analysis.},

  doi          = {10.15121/1452718},

  journal      = {},

  number       = ,

  volume       = ,

  place        = {United States},

  year         = {Thu Jun 01 00:00:00 EDT 2017},

  month        = {Thu Jun 01 00:00:00 EDT 2017}

}

Copy to clipboard

Dataset:

View Dataset

DOI: https://doi.org/10.15121/1452718

Save / Share:

Export Metadata

Save to My Library

Similar records in DOE Data Explorer and OSTI.GOV collections:

Rare Earth Adsorption and Desorption with PEGDA Beads

Dataset Jiao, Yongqin ; Brewer, Aaron ; Park, Dan

We synthesized PEGDA polymer hydrogel beads for cell embedding and compared REE biosorption with these beads via a gravity-driven flow through setup. One way to set up a flow through system is by cell encapsulation into polymer beads with a column setup similar to that used in the chromatography industry. To achieve this, we tested PEGDA for cell encapsulation, and tested REE biosorption under both batch mode and a follow through setup based on gravity . For making the cell embedded polymer beads, we used a fluidic device by which homogenous spherical particles of 0.5 to1 mm in diameter weremore »« less
Google Earth Locations of USA and Seafloor Hydrothermal Vents with Associated Rare Earth Element Data

Dataset Fowler, Andrew

Google Earth .kmz files that contain the locations of geothermal wells and thermal springs in the USA, and seafloor hydrothermal vents that have associated rare earth element data. The file does not contain the actual data, the actual data is available through the GDR website in two tier 3 data sets entitled "Compilation of Rare Earth Element Analyses from US Geothermal Fields and Mid Ocean Ridge (MOR) Hydrothermal Vents" and "Rare earth element content of thermal fluids from Surprise Valley, California"
Fluid rare earth element anlayses from wells RN-12 and RN-19, Reykjanes, Iceland

Dataset Fowler, Andrew

Results for fluid rare earth elment analyses from Reykjanes wells RN-12 and RN-19. The data have not been corrected for flashing. Samples preconcetrated using chelating resin with IDA functional group (InertSep ME-1). Analyzed using and Element magnetic sctor ICP-MS.
Compilation of Rare Earth Element Analyses from US Geothermal Fields and Mid Ocean Ridge Hydrothermal Vents

Dataset Fowler, Andrew

Compilation of rare earth element and associated major and minor dissolved constituent analytical data for USA geothermal fields and global seafloor hydrothermal vents. Data is in original units. Reference to and use of this data should be attributed to the original authors and publications according to the provisions outlined therein.
Column Sorption Uptake and Regeneration Study; Rare Earth Element Sorbent Uptake and Sorbent Stripping

Dataset Lanyk, Tim

Study of rare earth element (REE) uptake from geothermal brine simulant by column loading, metal recovery through stripping, and regeneration of column for re-loading. Simulated brine testing.

Similar Records