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Title: Rare Earth Desorption Test with Citrate and Bicarbonate

LBT (lanthanide binding tag) cells were grown overnight in LB media with 0.05% Amp. 1:100 subculture taken from overnights, grown for 2 hours. LBT was induced with 0.002% arabinose added for 3 hours. REE adsorption was done by combining 350 ul (0.25% 1M MES, 12.5 uM Tb, and 12.5 uM La or Cu in sterile DI water) and 350 ul (LBT cells with OD = 1 in 10 mM MES), reacted for approx. 30 min. Following adsorption, citrate and bicarbonate solutions were used in desorption to recover rare earth from cell surface, and to further separate REE from non-REEs. The samples were then centrifuged and a fraction of the supernatant was collected for ICP-MS analysis.
Authors:
; ;
Publication Date:
Report Number(s):
965
DOE Contract Number:
LLNL FY17 AOP 2.5.1.12
Product Type:
Dataset
Research Org(s):
DOE Geothermal Data Repository; Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
Collaborations:
Lawrence Livermore National Laboratory
Sponsoring Org:
USDOE Office of Energy Efficiency and Renewable Energy (EERE), Geothermal Technologies Program (EE-2C)
Geolocation:
POLYGON ((-118.3872640625 39.295415290912,-118.3872640625 35.15607354867,-122.2433640625 35.15607354867,-122.2433640625 39.295415290912,-118.3872640625 39.295415290912))
Subject:
15 Geothermal Energy; geothermal; energy; adsorption; desorption; rare earth; citrate; bicarbonate; REE; resource recovery; centrifuge; brine; lbt; lanthanide binding tag
Related Identifiers:
OSTI Identifier:
1452718

Jiao, Yongqin, Brewer, Aaron, and Park, Dan. Rare Earth Desorption Test with Citrate and Bicarbonate. United States: N. p., Web. doi:10.15121/1452718.
Jiao, Yongqin, Brewer, Aaron, & Park, Dan. Rare Earth Desorption Test with Citrate and Bicarbonate. United States. doi:10.15121/1452718.
Jiao, Yongqin, Brewer, Aaron, and Park, Dan. 2017. "Rare Earth Desorption Test with Citrate and Bicarbonate". United States. doi:10.15121/1452718. https://www.osti.gov/servlets/purl/1452718.
@misc{osti_1452718,
title = {Rare Earth Desorption Test with Citrate and Bicarbonate},
author = {Jiao, Yongqin and Brewer, Aaron and Park, Dan},
abstractNote = {LBT (lanthanide binding tag) cells were grown overnight in LB media with 0.05% Amp. 1:100 subculture taken from overnights, grown for 2 hours. LBT was induced with 0.002% arabinose added for 3 hours. REE adsorption was done by combining 350 ul (0.25% 1M MES, 12.5 uM Tb, and 12.5 uM La or Cu in sterile DI water) and 350 ul (LBT cells with OD = 1 in 10 mM MES), reacted for approx. 30 min. Following adsorption, citrate and bicarbonate solutions were used in desorption to recover rare earth from cell surface, and to further separate REE from non-REEs. The samples were then centrifuged and a fraction of the supernatant was collected for ICP-MS analysis.},
doi = {10.15121/1452718},
year = {2017},
month = {6} }
  1. The Geothermal Data Repository (GDR) is the submission point for all data collected from researchers funded by the U.S. Department of Energy's Geothermal Technologies Office (DOE GTO). The DOE GTO is providing access to its geothermal project information through the GDR. The GDR is powered by OpenEI, an energy information portal sponsored by the U.S. Department of Energy and developed by the National Renewable Energy Laboratory (NREL).
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